{"product_id":"proteintech-87148-1-rr","title":"Proteintech, 87148-1-RR, Phospho-RIPK3 (Ser232) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-RIPK3 (Ser232) (87148-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RIPK3 (Ser232) in WB, ELISA applications with reactivity to human, mouse samples\n87148-1-RR targets Phospho-RIPK3 (Ser232) in WB, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: TNF alpha, Z-VAD and SM-164 treated L-929 cells, TNF alpha,  Z-VAD and SM-164 treated L-929 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRIPK3,also named as RIP3, a Ser\/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. TNF was found to induce the phosphorylation of Thr-231 and Ser-232 in mouse RIP3 (mRIP3), and this phosphorylation is required for the interaction between mRIP3 and mMLKL. Ser-232 in mRIP3 corresponds to Ser-227 in hRIP3, whereas Thr-231 is not conserved in hRIP3. (PMID: 23612963) This antibody specifically recognizes phosphorylation of the mouse RIPK3 protein at Ser232.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: receptor-interacting serine-threonine kinase 3\nCalculated Molecular Weight: 53 kDa\nObserved Molecular Weight: 46-62 kDa\nGenBank Accession Number: NM_019955\nGene Symbol: Ripk3\nGene ID (NCBI): 56532\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9QZL0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888804286633,"sku":"87148-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-87148-1-rr","provider":"Iright","version":"1.0","type":"link"}