{"product_id":"proteintech-98262-1-rr","title":"Proteintech, 98262-1-RR, Anti-GGGGS Linker Rabbit Recombinant Antibody","description":"Size: 100ug\nThe GGGGS Linker (98262-1-RR) by Proteintech is a Recombinant antibody targeting GGGGS Linker in WB, IHC, IF\/ICC, FC applications with reactivity to recombinant protein samples\n98262-1-RR targets GGGGS Linker in WB, IHC, IF\/ICC, FC applications and shows reactivity with recombinant protein samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Transfected HEK-293T cells\nPositive IHC detected in: Transfected HEK-293T cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Transfected HEK-293T cells, Transfected CHO-K1\nPositive FC detected in: Transfected HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:40000-1:160000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:2000-1:8000\nFlow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAs a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: recombinant protein\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: GGGGS Linker\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purfication\nStorage Buffer: PBS with 0.09% sodium azide, pH 7.3.\nStorage Conditions: Store at 2 - 8°C. Stable for one year after shipment.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888693727401,"sku":"98262-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-98262-1-rr","provider":"Iright","version":"1.0","type":"link"}