{"product_id":"revvity-62htgfbpeh","title":"Revvity, 62HTGFBPEH, HTRF Human and Mouse TGF-β1 Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nTGF beta 1 (Transforming Growth Factor Beta-1) is considered one of major cytokines involved in regulation of extracellular matrix (ECM) synsis and degradation, as well as a major profibrotic factor. TGF beta 1 pre-proprotein is processed to generate a latency-associated peptide (LAP) and a mature TGFß1 peptide, which remain associated through strong non-covalent interactions. An activation step (MMP2 cleavage or acid activation) is required to release active form of TGF beta 1 from TGFß1-LAP complex.\u003cbr\u003e\nAssessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.\u003cbr\u003e\nHow it works\u003cbr\u003e\nActivation step\u003cbr\u003e\nThe samples and cell culture supernatants contain TGF beta 1 and a TGFß1-LAP complex, which require an acid activation step followed by a neutralization in order to be detected (sample activation can follow one of the protocols described on the right). The reagents needed for this activation step are provided in the kit for maximum convenience. Standards and activated samples are dispensed directly into the detection (white, low-volume) plate for the detection by HTRF® reagents.\u003cbr\u003e\n \u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay principle\u003cbr\u003e\nCell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536 well-format by simply resizing each addition volume proportionally. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Cytokines\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Metabolism\/Diabetes NASH\/Fibrosis Oncology \u0026amp; Inflammation\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775321985193,"sku":"62HTGFBPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/revvity-62htgfbpeh","provider":"Iright","version":"1.0","type":"link"}