{"product_id":"revvity-64tespeg","title":"Revvity, 64TESPEG, HTRF Testosterone Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nTestosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\nThe testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\n\n\n\n\u003cp\u003eAssay Protocol\u003cbr\u003e\nThe testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents This protocol requires a single one-hour incubation period following cell stimulation. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-10 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Metabolism\/Diabetes Neuroscience\u003cbr\u003e\nUnit Size-500 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775321952425,"sku":"64TESPEG","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/revvity-64tespeg","provider":"Iright","version":"1.0","type":"link"}