{"product_id":"thermo-fisher-20349","title":"Thermo Fisher, 20349, Pierce™ Streptavidin Agarose Thermo Scientific™","description":"\u003cp\u003ePierce™ Streptavidin Agarose Thermo Scientific™ Thermo Scientific Pierce Streptavidin Agarose is a standard-capacity beaded agarose resin of immobilized recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.\u003cbr\u003e\nFeatures of Streptavidin Agarose:\u003cbr\u003e\n•Streptavidin—purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule\u003cbr\u003e\n•Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods\u003cbr\u003e\n•Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield\u003cbr\u003e\n•Standard capacity—this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3 mg of biotinylated BSA per mL of resinProperties of crosslinked 6% beaded agarose (CL-6B):\u003cbr\u003e\n• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)\u003cbr\u003e\n• Average Particle Size: 45 to 165 microns\u003cbr\u003e\n• Exclusion Limit: 10,000 to 4,000,000 daltons\u003cbr\u003e\n• Maximum Volumetric Flow Rate: approx. 1 mL\/minute (for 1 cm diameter column)\u003cbr\u003e\n• Maximum Linear Velocity: 30 cm per hour\u003cbr\u003e\n• Maximum Pressure: less than 25psi (1.\u003cbr\u003e\n5 bar)Applications:\u003cbr\u003e\n• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies\u003cbr\u003e\n• Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent\u003cbr\u003e\n• Isolation of cell-surface glycoproteins using biotinylated Concanavalin A\u003cbr\u003e\n• Recovery of single-stranded biotinylated DNA for dideoxy sequencing\u003cbr\u003e\n• Streptavidin-coated plates used for direct solid-phase sequencing\u003cbr\u003e\n•In vitromutagenesis with streptavidin-coated plates\u003cbr\u003e\n• DNA fingerprinting with streptavidin-coated platesPierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.\u003cbr\u003e\nStreptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein fromStreptomyces avidiniiis carbohydrate-free, has an acidic pI of 5.\u003cbr\u003e\n5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.\u003cbr\u003e\nColumn Type: Affinity, Agarose Resin\u003cbr\u003e\nFormat: Bottle\u003cbr\u003e\nProtein Form: Recombinant\u003cbr\u003e\nQuantity: 5 mL\u003cbr\u003e\nStationary Phase: Streptavidin\u003cbr\u003e\nSufficient For: Binding 15 to 28 μg Biotin\/mL Resin (1 to 3 mg Biotinylated BSA\/mL Resin)\u003cbr\u003e\nTarget: Biotin-Tagged Molecule\u003cbr\u003e\nCapacity (Metric): 1 to 3 mg Biotinylated BSA Binding per mL Resin\u003cbr\u003e\nParticle Size: 45 to 165 μm\u003cbr\u003e\nProduct Line: Pierce™\u003cbr\u003e\nType: Pre-packed Column\u003cbr\u003e\nUnit Size: Each\u003c\/p\u003e","brand":"Thermo Fisher","offers":[{"title":"Default Title","offer_id":45789004169385,"sku":"20349","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/thermo-fisher-20349","provider":"Iright","version":"1.0","type":"link"}