{"product_id":"thermo-fisher-78840","title":"Thermo Fisher, 78840, Subcellular Protein Fractionation Kit for Cultured Cells Thermo Scientific™","description":"\u003cp\u003eSubcellular Protein Fractionation Kit for Cultured Cells Thermo Scientific™ Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.\u003cbr\u003e\nFeatures of Subcellular Protein Fractionation Kit for Cultured Cells:\u003cbr\u003e\n•Concise—extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions from mammalian cultured cells in less than 3 hours\u003cbr\u003e\n•Convenient—perform a simple procedure without using ultracentrifugation over gradients\u003cbr\u003e\n•Complete—obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit\u003cbr\u003e\n•Cultured Cells—developed and optimized for use with cultured mammalian cells; for tissue samples use theSubcellular Fractionation Kit for Tissues\u003cbr\u003e\n•Compatible—use extracts for downstream applications such as protein assays, Western blotting, protein assays, electrophoretic mobility shift assays, reporter-gene and enzyme-activity assays.\u003cbr\u003e\nApplications for subcellular fractionation:\u003cbr\u003e\n• Extract and enrich proteins from different cellular compartments\u003cbr\u003e\n• Isolate cytoplasmic, nuclear, membrane and cytoskeletal proteins from the same cells\u003cbr\u003e\n• Profile cells for proteins from the cytoplasm, nucleus, membrane and cytoskeleton\u003cbr\u003e\n• Study protein translocationFractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.\u003cbr\u003e\nThe Subcellular Protein Fractionation Kit for Cultured Cells contains four extraction buffers, a stabilized nuclease and Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has enough reagents to fractionate 50 cell pellets of 20 μL each, equivalent to approximately 2g of cell paste. (For tissue samples, use ourSubcellular Protein Fractionation Kit for Tissue.)The first kit reagent, when added to a cell pellet, causes selective membrane permeabilization, releasing soluble cytoplasmic contents. The second reagent dissolves plasma, mitochondria and ER-golgi membranes but does not solubilize the nuclear membranes. After recovering intact nuclei by centrifugation, a third reagent yields the soluble nuclear extract. An additional nuclear extraction with micrococcal nuclease is performed to release chromatin-bound nuclear proteins. The recovered insoluble pellet is then extracted with the final reagent to isolate cytoskeletal proteins.\u003cbr\u003e\nBuffer: Lysis Buffer\u003cbr\u003e\nQuantity: 1 Kit\u003cbr\u003e\nReagent Type: Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation\u003cbr\u003e\nSufficient For: 50 Samples\u003cbr\u003e\nForm: Liquid\u003cbr\u003e\nProduct Type: Subcellular Protein Fractionation Kit\u003cbr\u003e\nUnit Size: Each\u003c\/p\u003e","brand":"Thermo Fisher","offers":[{"title":"Default Title","offer_id":45788887154857,"sku":"78840","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/thermo-fisher-78840","provider":"Iright","version":"1.0","type":"link"}