{"product_id":"thermo-fisher-en0521","title":"Thermo Fisher, EN0521, DNase I, RNase-free (1 U\/µL) ","description":"\u003cp\u003eDNase I, RNase-free (1 U\/µL)  Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.\u003cbr\u003e\nThe enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.\u003cbr\u003e\nIn the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.\u003cbr\u003e\nHighlights\u003cbr\u003e\n• Recombinant enzyme\u003cbr\u003e\n• Purified from non-animal host with a lower level of intrinsic RNasesApplications\u003cbr\u003e\n• Preparation of DNA-free RNA\u003cbr\u003e\n• Removal of template DNA following in vitro transcription\u003cbr\u003e\n• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR\u003cbr\u003e\n• DNA labeling by nick-translation in conjunction with DNA Polymerase I\u003cbr\u003e\n• Studies of DNA-protein interactions by DNase I, RNase-free footprinting\u003cbr\u003e\n• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is usedNoteDNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.\u003cbr\u003e\nCompatible Buffer: Reaction Buffer\u003cbr\u003e\nProduct Type: DNase I RNase Free\u003cbr\u003e\nQuantity: 1,000 units\u003cbr\u003e\nConcentration: 1 U\/μL\u003cbr\u003e\nEnzyme: DNase\u003cbr\u003e\nUnit Size: Each\u003c\/p\u003e","brand":"Thermo Fisher","offers":[{"title":"Default Title","offer_id":45789431300265,"sku":"EN0521","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/thermo-fisher-en0521","provider":"Iright","version":"1.0","type":"link"}