Agilent Chromatography Columns

Agilent Chromatography Columns are high-performance liquid and gas chromatography columns designed for precision separation and identification of chemical compounds. This category includes Agilent's full range of HPLC, UHPLC, and GC columns—each engineered for exceptional analytical accuracy, reproducibility, and reliability.

These columns are widely trusted in regulated industries for their quality, batch-to-batch consistency, and broad application compatibility. Whether you are analyzing small molecules, large biomolecules, or volatile organic compounds, Agilent offers a robust portfolio of columns to meet your analytical demands.

Key Advantages of Agilent Chromatography Columns

Superior Separation Efficiency
Agilent columns are designed to deliver tight, symmetrical peaks with excellent resolution, making them ideal for separating complex sample matrices—even those with closely related compounds or low abundance analytes.

Unmatched Batch Reproducibility
Agilent employs strict manufacturing controls and quality standards to ensure consistent column performance across batches. This reproducibility is critical in regulated labs performing validation or routine testing.

Broad Compatibility and Versatility
The column portfolio supports a wide variety of separation modes—reversed-phase, normal-phase, ion exchange, size exclusion, and more. They are also available in diverse formats to fit HPLC, UHPLC, GC, and specialty systems.

Long Column Lifespan
Built with durable packing materials and column hardware, Agilent columns maintain performance under high pressures, varying pH conditions, and aggressive solvents, providing a longer service life and lower replacement cost.

Global Recognition and Support
Backed by decades of chromatography innovation, Agilent columns are supported by comprehensive technical documentation, application notes, and a global support network—trusted by scientists worldwide.

Typical Applications & Use Cases

Agilent chromatography columns serve critical roles in a broad range of scientific and industrial workflows:

• Pharmaceutical Quality Control and R&D
  Used in API assay, impurity profiling, and dissolution testing. Agilent columns help meet stringent FDA, USP, and ICH requirements.
• Biopharmaceutical Characterization
  Specialized columns for peptides, mAbs, oligonucleotides, and glycan analysis. Support for SEC, RP, and HILIC separations.
• Environmental Testing
  Columns optimized for trace-level detection of pesticides, VOCs, PAHs, and regulated contaminants in water, air, and soil.
• Food and Beverage Safety
  Detection of food additives, preservatives, flavor compounds, residual solvents, and contaminants using both LC and GC methods.
• Chemical and Industrial Manufacturing
  Applied in petrochemical analysis, purity verification, and process monitoring for specialty chemicals and intermediates.

FAQs

Q1: How do I decide between HPLC and UHPLC columns?

If your system supports high-pressure operation (typically >600 bar), UHPLC columns offer faster run times, better resolution, and lower solvent usage. HPLC columns are better suited for legacy instruments or standard workflows.

Q2: What should I consider when selecting a chromatography column?

Consider these key parameters:

• Column chemistry (e.g., C18, Phenyl-Hexyl, HILIC)
• Particle size and pore size
• Column dimensions (length × diameter)
• Application (target compounds and sample matrix)

Q3: Can I use Agilent columns with Waters, Shimadzu, or Thermo systems?

Yes. Agilent columns are manufactured to industry-standard dimensions and can be used interchangeably with most major LC and GC instruments.