{"title":"BD Flow Cytometry Reagents for Multicolor Panels","description":"\u003cp\u003eFlow cytometry lives or dies on signal quality and reproducibility. This Collection brings together BD Biosciences reagents—antibodies, viability dyes, buffers, compensation beads, and fix\/perm solutions—so you can design cleaner multicolor panels, reduce staining artifacts, and move from experiment plan to gated results with fewer surprises. As a distributor, Iright offers a wide selection of BD flow reagents with reliable sourcing, invoicing, and technical support.\u003c\/p\u003e\n\u003ch2\u003eWhy BD Flow Cytometry Reagents Matter at Iright\u003c\/h2\u003e\n\u003cp\u003eBefore diving into product families, it helps to clarify what you gain: consistent staining across complex panels, options to balance brightness against antigen abundance, and utilities that tame compensation headaches. With Iright, you can source the right BD reagent mix, get SKU-level accuracy, and keep your panel design workflow simple.\u003c\/p\u003e\n\u003cp\u003eBD’s portfolio aims at real panel pain points: higher-brightness fluorochromes for rare targets, buffers that mitigate staining artifacts when multiple Brilliant dyes are used, and standardized kits for intracellular staining. These elements shorten the path from panel idea to interpretable gates.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-flow-cytometry-reagents.avif?v=1761808141\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch2\u003eProduct Families at a Glance: Antibodies, Dyes, Buffers \u0026amp; Beads\u003c\/h2\u003e\n\u003cp\u003eA clear map helps you navigate fast. Use this overview to locate the reagent type you need, then jump to selection tips and example use cases. Each family supports a specific stage of your staining workflow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry antibodies (BD Horizon™, BD Pharmingen™, BD Horizon Brilliant™)\u003c\/strong\u003e: Broad target coverage (e.g., CD markers) with multiple laser lines and brightness tiers for panel flexibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eViability dyes\u003c\/strong\u003e: Separate live\/dead events to reduce false positives and clean up gates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffers \u0026amp; supporting reagents\u003c\/strong\u003e: \u003cstrong\u003eBD Horizon™ Brilliant Stain Buffer (BSB)\u003c\/strong\u003e and \u003cstrong\u003eBSB Plus\u003c\/strong\u003e help mitigate staining artifacts with multiple Brilliant dyes and support low-volume staining workflows.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFixation\/Permeabilization\u003c\/strong\u003e: \u003cstrong\u003eBD Cytofix\/Cytoperm™ Kit\u003c\/strong\u003e standardizes intracellular staining of cytokines and transcription factors while preserving surface markers.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompensation \u0026amp; unmixing beads: BD™ CompBeads\u003c\/strong\u003e and SpectraComp™ particles enable a reproducible compensation and spectral unmixing setup.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptiBuild™ on-demand reagents\u003c\/strong\u003e: Expand your color\/clone combinations to add markers or reduce compensation burden.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-cytofic-cytoperm-kit.webp?v=1761808246\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch2\u003eHow These Reagents Improve Multicolor Panel Performance\u003c\/h2\u003e\n\u003cp\u003eNow that you’ve seen the lineup, let’s connect products to outcomes. This section focuses on practical gains—resolution, stability, and panel scalability—so your design decisions are driven by effect, not guesswork.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBrighter signals and clearer separation.\u003c\/strong\u003e BD Horizon Brilliant fluorochromes provide high brightness across common lasers, improving rare-population resolution and enabling cleaner bivariate gating. Pair high-brightness colors with low-abundance antigens to lift weak signals out of the background.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFewer staining artifacts in complex panels.\u003c\/strong\u003e When using multiple Brilliant dyes, \u003cstrong\u003eBrilliant Stain Buffer\u003c\/strong\u003e and BSB Plus help mitigate artifacts and preserve expected expression patterns. BSB Plus keeps the mitigation benefits while supporting reduced staining volumes, which is useful when the sample or reagent is limited.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReliable intracellular workflows.\u003c\/strong\u003e \u003cstrong\u003eCytofix\/Cytoperm\u003c\/strong\u003e provides a standardized fix\/perm process and includes Perm\/Wash for consistent intracellular staining results without sacrificing your surface staining strategy.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReproducible compensation and unmixing.\u003c\/strong\u003e \u003cstrong\u003eCompBeads\u003c\/strong\u003e and SpectraComp particles deliver consistent positive\/negative controls to set compensation matrices or spectral unmixing, reducing run-to-run variability.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible color choices.\u003c\/strong\u003e \u003cstrong\u003eOptiBuild\u003c\/strong\u003e on-demand reagents open additional dye–clone combinations so you can add markers or shift colors to cleaner channels as your panel evolves.\u003c\/p\u003e\n\u003ch2\u003eSelection Guide: Flow Cytometry Panel Design Basics\u003c\/h2\u003e\n\u003cp\u003eGood panels match color brightness to antigen biology and instrument reality. Use this quick guide as a checklist when you assemble or expand a panel—especially beyond 8–10 colors.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatch brightness to abundance:\u003c\/strong\u003e Assign the brightest dyes (often Brilliant series) to low or variable expression targets; reserve moderate brightness for high-abundance antigens.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDistribute across lasers:\u003c\/strong\u003e Spread colors over 355\/405\/488\/561\/640 nm to reduce spectral overlap and minimize compensation load.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLock down “must-have” channels first:\u003c\/strong\u003e Place critical functional markers on the cleanest detectors; fill supporting markers afterward.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlan controls early:\u003c\/strong\u003e Include single-colors, FMO\/negative controls, viability dye, and compensation\/unmixing beads. If your panel uses multiple Brilliant dyes, add BSB\/BSB Plus to the workflow from the start.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStandardize intracellular steps:\u003c\/strong\u003e For cytokines\/transcription factors, follow Cytofix\/Cytoperm protocol and keep Perm\/Wash conditions consistent across runs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatured Use Cases: T-Cell, B-Cell \u0026amp; Intracellular Cytokines\u003c\/h2\u003e\n\u003cp\u003eExamples help you translate principles into carts. Treat these as modular patterns you can adapt to your markers and instruments.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHuman T-cell subsets:\u003c\/strong\u003e CD3, CD4, CD8, CCR7, CD45RA\/RO, activation\/exhaustion markers; add a viability dye and include \u003cstrong\u003eBSB\u003c\/strong\u003e if multiple Brilliant dyes are present to stabilize multicolor staining.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eB-cell profiling: \u003c\/strong\u003eCD19, CD27, IgD, CD38 with a viability dye; choose higher brightness for rarer populations and standard brightness for bulk lineage markers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntracellular cytokines (e.g., IFN-γ, IL-2):\u003c\/strong\u003e Perform surface stain, then \u003cstrong\u003eCytofix\/Cytoperm\u003c\/strong\u003e fixation\/permeabilization and intracellular staining; set compensation using \u003cstrong\u003eCompBeads\u003c\/strong\u003e or SpectraComp as appropriate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePopular BD Lines: Horizon, Brilliant Stain Buffer, Cytofix\/Cytoperm, OptiBuild\u003c\/h2\u003e\n\u003cp\u003eAs you refine your panel, you’ll encounter these names frequently. Understanding what each line does helps you select intentionally and avoid trial-and-error.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBD Horizon™ \/ BD Horizon Brilliant™ antibodies:\u003c\/strong\u003e Broad clone coverage with high-brightness options for critical or low-abundance targets; designed for modern multicolor cytometers.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBrilliant Stain Buffer (BSB) \u0026amp; BSB Plus:\u003c\/strong\u003e BSB (Cat. \u003ca href=\"https:\/\/iright.com\/products\/bd-563794\" rel=\"noopener\" target=\"_blank\"\u003eNo. 563794\u003c\/a\u003e, 100 tests; \u003ca href=\"https:\/\/iright.com\/products\/bd-566349\" rel=\"noopener\" target=\"_blank\"\u003e566349\u003c\/a\u003e, 1000 tests) mitigates staining artifacts with multiple Brilliant dyes; BSB Plus supports reduced staining volumes while maintaining mitigation performance (e.g., Cat. No. \u003ca href=\"https:\/\/iright.com\/products\/bd-568264\" rel=\"noopener\" target=\"_blank\"\u003e568264\u003c\/a\u003e, 100 tests).\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCytofix\/Cytoperm™ Fixation\/Permeabilization:\u003c\/strong\u003e Standardized kit for intracellular targets (Kit Cat. No. \u003ca href=\"https:\/\/iright.com\/products\/bd-554714\" rel=\"noopener\" target=\"_blank\"\u003e554714\u003c\/a\u003e; components include \u003ca href=\"https:\/\/iright.com\/products\/bd-554722\" rel=\"noopener\" target=\"_blank\"\u003e554722\u003c\/a\u003e Cytofix\/Cytoperm and \u003ca href=\"https:\/\/iright.com\/products\/bd-554723\" rel=\"noopener\" target=\"_blank\"\u003e554723\u003c\/a\u003e Perm\/Wash).\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompensation beads:\u003c\/strong\u003e Anti-Mouse Igκ CompBeads (Cat. No. \u003ca href=\"https:\/\/iright.com\/products\/bd-552843\" rel=\"noopener\" target=\"_blank\"\u003e552843\u003c\/a\u003e) and CompBead Plus Anti-Mouse Igκ (Cat. No. \u003ca href=\"https:\/\/iright.com\/products\/bd-560497\" rel=\"noopener\" target=\"_blank\"\u003e560497\u003c\/a\u003e) provide consistent positive and negative bead populations for compensation setup.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptiBuild™ on-demand reagents:\u003c\/strong\u003e Access additional dye–clone combinations; BD is also converting popular OptiBuild items to off-the-shelf formats for easier procurement.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eComparison Table: Use, Advantages \u0026amp; Pairing for BD Flow Reagents\u003c\/h2\u003e\n\u003cp\u003eThis table summarizes where each category fits in your workflow. Use it to spot gaps, avoid redundant steps, and pair reagents effectively.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eCategory\u003c\/th\u003e\n\u003cth\u003eTypical Use\u003c\/th\u003e\n\u003cth\u003eKey Advantages\u003c\/th\u003e\n\u003cth\u003ePair With\u003c\/th\u003e\n\u003cth\u003eNotes \/ Representative SKUs\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAntibodies (Horizon\/Brilliant\/Pharmingen)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSurface or intracellular (with fix\/perm)\u003c\/td\u003e\n\u003ctd\u003eWide clone \u0026amp; color coverage; high brightness for rare targets\u003c\/td\u003e\n\u003ctd\u003eBSB\/BSB Plus; viability dye; CompBeads\u003c\/td\u003e\n\u003ctd\u003eChoose brightness by antigen abundance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eViability Dyes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLive\/dead discrimination\u003c\/td\u003e\n\u003ctd\u003eCleaner gates; fewer false positives\u003c\/td\u003e\n\u003ctd\u003eAny panel\u003c\/td\u003e\n\u003ctd\u003eAdd early; keep timing consistent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBrilliant Stain Buffer (BSB)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePanels using ≥2 Brilliant dyes\u003c\/td\u003e\n\u003ctd\u003eMitigates staining artifacts in complex panels\u003c\/td\u003e\n\u003ctd\u003eBrilliant-conjugated antibodies\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e563794\u003c\/strong\u003e (100 tests), \u003cstrong\u003e566349\u003c\/strong\u003e (1000 tests)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBrilliant Stain Buffer Plus\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLow-volume staining with Brilliant dyes\u003c\/td\u003e\n\u003ctd\u003eArtifact mitigation at reduced volumes\u003c\/td\u003e\n\u003ctd\u003eBrilliant-conjugated antibodies\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e568264\u003c\/strong\u003e (100 tests)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCytofix\/Cytoperm Kit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIntracellular cytokines\/transcription factors\u003c\/td\u003e\n\u003ctd\u003eStandardized fix\/perm; preserves surface staining strategy\u003c\/td\u003e\n\u003ctd\u003eIntracellular antibody cocktails\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e554714\u003c\/strong\u003e kit; includes \u003cstrong\u003e554722\u003c\/strong\u003e, \u003cstrong\u003e554723\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompensation \/ Spectral Beads\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCompensation matrix \/ unmixing controls\u003c\/td\u003e\n\u003ctd\u003eReproducible positive\/negative populations\u003c\/td\u003e\n\u003ctd\u003eAny multicolor panel\u003c\/td\u003e\n\u003ctd\u003e\n\u003cstrong\u003e552843\u003c\/strong\u003e (CompBeads Mouse), \u003cstrong\u003e560497\u003c\/strong\u003e (CompBead Plus Mouse)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eFAQs: BSB vs BSB Plus, Fix\/Perm, Compensation Beads \u0026amp; Viability Dyes\u003c\/h2\u003e\n\u003cp\u003eMany panel issues repeat across labs. These answers focus on practical, searchable questions so you can troubleshoot quickly and choose with confidence.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat’s the difference between BSB and BSB Plus?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eBoth mitigate staining artifacts when multiple Brilliant dyes are used; BSB Plus is formulated for reduced staining volumes without sacrificing mitigation. Choose BSB for standard volumes and BSB Plus when sample or reagent is limited.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHow do I set up intracellular staining properly?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStain surface markers first, then use the Cytofix\/Cytoperm Kit to fix and permeabilize, followed by intracellular antibodies in Perm\/Wash buffer. Keep timing and temperatures consistent to protect epitope integrity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhich beads should I use for compensation or spectral unmixing?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUse BD CompBeads to generate consistent positive\/negative populations for traditional compensation. For spectral systems, SpectraComp™ particles provide unmixing controls across multiple species. Always run single-color controls that match your fluorochromes.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDo I need a viability dye in every panel?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIf your sample prep risks dead cells (most do), a viability dye is strongly recommended. It reduces background, improves gating accuracy, and stabilizes population frequencies across replicates.\u003c\/p\u003e\n\u003ch2\u003eTalk to Iright\u003c\/h2\u003e\n\u003cp\u003eWhether you are expanding to a 14-color panel or standardizing an intracellular protocol, we can help you choose the right antibodies, buffers, BSB\/BSB Plus, fix\/perm kits, and compensation beads—then deliver them reliably. Share your cytometer\/laser setup, target list, and test volume; we’ll suggest suitable dye–clone options (including OptiBuild when helpful), confirm BD SKUs, and align lead times with your schedule.\u003c\/p\u003e\n\u003cdiv class=\"product-full-width\"\u003e\n\u003cdiv class=\"product-block\"\u003e\n\u003ch3\u003eOrder Guidelines\u003c\/h3\u003e\n\u003cp\u003e1. Price \u0026amp; Stock Available on Request. \u003cb\u003e\u003ca href=\"mailto:service@iright.com\"\u003eClick to send email to: service@iright.com\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e2. Please DO NOT make any payment before confirmation.\u003c\/p\u003e\n\u003cp\u003e3. Minimum order value of $1,000 USD required.\u003c\/p\u003e\n\u003cp\u003e4. 100% prepayment required.\u003c\/p\u003e\n\u003ch3\u003eCollaboration\u003c\/h3\u003e\n\u003cp\u003eTony Tang\u003c\/p\u003e\n\u003cp\u003eEmail: Tony.Tang@iright.com\u003c\/p\u003e\n\u003cp\u003eMobile\/WhatsApp\/Wechat: +86-17717886924\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","products":[{"product_id":"bd-554714","title":"BD, 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Kit","description":"\u003cp\u003eRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869008\u003cbr\u003e\nDescription: Description This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation\/permeabilization solution and BD Perm\/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm\/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 125.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400252073,"sku":"554714","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-556547","title":"BD, 556547, BD Pharmingen™ FITC Annexin V Apoptosis Detection Kit I","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869082\u003cbr\u003e\nDescription: Description Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V positive). Viable cells with intact membranes exclude PI, wheras the membranes of dead and damaged cells are permeable to PI. For example, cells that are considered viable are FITC Annexin V and PI negative; cells that are in early apoptosis are FITC Annexin V positive and PI negative; and cells that are in late apoptosis or already dead are are both FITC Annexin V and PI positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both FITC Annexin V and PI. However, when apoptosis is measured over time, cells can be often tracked from FITC Annexin V and PI negative (viable, or no measurable apoptosis), to FITC Annexin V positive and PI negative (early apoptosis, membrane integrity is present) and finally to FITC Annexin V and PI positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both FITC Annexin V and PI positive, in of itself, reveals less information about the process by which the cells underwent their demise.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining as described in the FITC Annexin V Staining Protocol. Investigators should note that FITC Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however, have been previously reported (Casiola-Rosen et al . and van Engelend et al.). INDUCTION OF APOPTOSIS BY CAMPTOTHECIN The following protocol is provided as an illustration on how FITC Annexin V may be used on a cell line (Jurkat). Materials 1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 µM) to 1 x 10^6 Jurkat cells. 2. Incubate the cells for 4-6 hr at 37°C. 3. Proceed with the FITC Annexin V Staining Protocol to measure apoptosis. FITC ANNEXIN V STAINING PROTOCOL FITC Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment.   Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Propidium Iodide (PI) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Cells that stain positive for FITC Annexin V and negative for PI are undergoing apoptosis. Cells that stain positive for both FITC Annexin V and PI are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both FITC Annexin V and PI are alive and not undergoing measurable apoptosis. Reagents 1. FITC Annexin V (component no. 51-65874X):  Use 5 µl per test. 2. Propidium Iodide (PI) (component no. 51-66211E) is a convenient, ready-to-use nucleic acid dye.  Use 5 µl per test. 3. 10X Annexin V Binding Buffer (component no. 51-66121E):  0.1 M Hepes\/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2.  For a 1X working solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water. Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells\/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. Add 400 µl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr. SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY The following controls are used to set up compensation and quadrants: 1.  Unstained cells. 2.  Cells stained with FITC Annexin V (no PI). 3.  Cells stained with PI (no FITC Annexin V). Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with FITC Annexin V and\/or FITC Annexin V and PI. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (FITC Annexin V positive, PI negative or FITC Annexin V positive, PI positive). The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for FITC Annexin V. Thus the assay does not distinguish between cells that have already undergone an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with both FITC Annexin V and PI.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 2.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400284841,"sku":"556547","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564219","title":"BD, 564219, BD Pharmingen™ Human BD Fc Block™","description":"\u003cp\u003eReactivity: Human (QC Testing), Rhesus (Tested in Development)\u003cbr\u003e\nIsotype: Human IgG1\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_2728082\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Incubate 1 million cells suspended in 50-100 µL of staining buffer with 2.5 µg of Human BD Fc Block™ (10 minutes at room temperature) followed by staining with the desired fluorescent antibody. No washing step is needed between the blocking and staining steps.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA\/LE (No Azide\/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400317609,"sku":"564219","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-559925","title":"BD, 559925, BD Pharmingen™ 7-AAD","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400415913,"sku":"559925","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560779","title":"BD, 560779, BD Horizon™ V500 Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_1937324\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V500 under optimum conditions, and unreacted BD Horizon™ V500 was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. BD Horizon V500 has a maximum absorption of 415 nm and maximum emission of 500 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon V500 is covered by one or more of the following US patents: 8,431,416. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400481449,"sku":"560779","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565388","title":"BD, 565388, BD Horizon™ Fixable Viability Stain 780","description":"\u003cp\u003eApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869673\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS780 dye powder and 180 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. Add 180 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Red laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD™ LSR II, BD LSRFortessa™, or BD Accuri™ C6) can be used. This dye can be read out of filters commonly used for APC-Cy™7 (eg, 780\/60). Fluorescence compensation is best achieved using cell samples of interest. When designing multicolor staining panels, please be aware of fluorescence spillover into the BD Horizon™ BUV737, BD Horizon™ BV786, and PE-Cy™7 (when read off the yellow-green laser) channels. Panels should be optimized to take this spillover into account. To reduce fluorescence spillover, we recommend titrating FVS780 and using the lowest possible dye concentration that provides adequate resolution of live and dead cell populations of interest. Procedure Fixable Viability Stain 780 labeling of cells 1. Prepare cells for flow cytometric staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1-10x10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of BD Horizon™ Fixable Viability Stain 780 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance, as different cell types and different applications can result in a wide degree of variability in staining. 5. Incubate the mixture for 10-15 minutes at room temperature protected from light. a. Optional: Alternatively, incubate mixtures at 37°C for 5-7 minutes or 2-8°C for 30-60 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentrations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (eg, FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 780 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (eg, Cat. No. 558050, BD Phosflow™ Perm Buffer III), intracellular cytokine staining (eg, Cat. No. 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Kit), or transcription factor staining (eg, Cat. No. 562574\/562725, BD Pharmingen™ Transcription Factor Buffer Set). 5. Apoptotic cells can show variable staining. We recommend co-staining with, eg, Annexin V FITC (Cat. No. 556419) if further analysis is desired for the apoptotic cells. Danger: Causes serious eye damage. Precuationary statements: Wear protective gloves\/protective clothing\/eye protection\/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CONTROL\/doctor. Dispose of contents\/containers in an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Cy is a trademark of GE Healthcare. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400514217,"sku":"565388","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564406","title":"BD, 564406, BD Horizon™ Fixable Viability Stain 510","description":"\u003cp\u003eReactivity: Human, Mouse (Tested in Development)\u003cbr\u003e\nApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869572\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS510 dye powder and 260 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; e.g. Sigma D2650) to room temperature. Add 260 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Violet laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD LSRFortessa™ or BD™ LSR II) can be used. This dye can be read out of filters commonly used for BD Horizon™ Brilliant Violet 510, BD Horizon™ V500, or AmCyan (e.g., 525\/50). Fluorescence compensation is best achieved using a sample of the cells of interest. Procedure Fixable Viability Stain 510 labeling of cells 1. Prepare cells for flow cytometry staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1x10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of the BD Horizon™ Fixable Viability Stain 510 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance, as different cell types and different applications can result in a wide degree of variability in staining. 5. Incubate the mixture for 15 minutes at room temperature protected from light. a. Optional: Incubate the cells and dye mixtures at 2-8°C for 30-60 minutes. Alternatively, incubate mixtures at 37°C for 5-7 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (e.g., FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 510 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (e.g., Cat. No. 558050, BD Phosflow™ Perm Buffer III), intracellular cytokine staining (e.g., Cat. No. 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeablization Kit), or transcription factor staining (e.g., Cat. No. 562574, BD Pharmingen™ Transcription Factor Buffer Set). 5. Apoptotic cells can show variable staining. We recommend co-staining with, e.g., Annexin V APC (Cat. No. 550475) if further analysis is desired for the apoptotic cells.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400579753,"sku":"564406","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561037","title":"BD, 561037, BD Pharmingen™ APC-Cy™7 Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_396774\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, thus maximizing its fluorescence emission intensity, minimizing residual emission from APC, and minimizing required electronic compensation in multilaser-laser flow cytometry systems. Note: Although every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-Cy7 conjugate. APC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036). For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400612521,"sku":"561037","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563794","title":"BD, 563794, BD Horizon™ Brilliant Stain Buffer","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Protocols for Multicolor Immunofluorescent Staining of Cells Using BD Horizon Brilliant Stain Buffer Multicolor Staining of Human Cell Samples in Tubes or 96-Well Plates Using Individual Staining Reagents 1. Add 50 μL of BD Horizon Brilliant Stain Buffer to all tubes or desired wells for the experiment Note: The 50 μL amount of Brilliant Stain Buffer per tube or per well does not depend on the final staining volume or amount of cells used per tube or number of BD fluorescent antibodies used in staining.  Although written for use with human cells, these protocols can readily be adapted for analyzing mouse cells or cells from other species, for example, by staining mouse cells at 4°C rather than at room temperature (RT). 2. Add each fluorescent reagent at the recommended volume per test (eg, 5 μL or 20 μL) and then proceed to either Protocol 1, 2, or 3. Protocol 1 for Staining Whole Blood Samples in Tubes a. Add 100 μL of human whole blood to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 mL of BD FACS™ Lysing Solution (Cat. No. 349202; 10 min) or BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899; 15 min) per tube and incubate protected from light at RT e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer, eg, BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or BD Pharmingen™ Stain Buffer (BSA) (Cat. No. 554657) g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 2 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in Tubes a. Add 100 μL of human cells to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 ml of stain\/wash buffer per tube e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 3 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in 96-well Plates Note:   When planning for staining in plates, the user must account for the volume of the BD Horizon Brilliant Stain Buffer used.  Although written for use with human cells, this 96-well plate-based protocol can readily be adapted for analyzing mouse cells or cells from other species. a. Add 50 μL of human cells to each well b. Incubate (30 min) protected from light at RT c. Wash by adding 100 μL of stain\/wash buffer d. Pellet cells by centrifugation (5 min) at 1400-1500 rpm e. Aspirate supernatants f. Resuspend pelleted cells by adding 250 μL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate supernatants i. Resuspend pelleted cells thoroughly with 150 μL stain\/wash buffer by pipetting the suspended cells several times j. Transfer well contents to tubes and add additional stain\/wash buffer to the tubes as desired for flow cytometric analysis Note: Alternatively, acquire samples for flow cytometric analysis from the plate directly Multicolor Staining of Human Cell Samples in Tubes or 96-Well Plates Using Cocktailed Staining Reagents Instead of adding staining reagents individually to each tube or well of a 96-well plate, it may be desirable to add cocktailed staining reagents, ie, mixtures of two or more fluorescent staining reagents. The following protocol provides an example of how to prepare a \"per test\" 5-Color Fluorescent Antibody Cocktail that already contains BD Horizon Brilliant Stain Buffer. Human Samples:  Pre-mixed Fluorescent Reagent Cocktails For each multicolor test of cocktailed fluorescent reagents: i)  Add 50 μL of BD Horizon Brilliant Stain Buffer per test ii)  Add each fluorescent reagent at the recommended volume per test (5 μL or 20 μL) iii)  Mix reagents (especially after adding BD Horizon Brilliant reagents) iv)  Store cocktail at 4°C protected from light if it is to be used later Note: Protected from light, fluorescent reagent cocktails containing more than one Brilliant Violet and\/or Brilliant Blue reagent are best used within 24 hours after preparation when stored at 4ºC or within 4 hours when stored at room temperature. However, when more than one Brilliant Ultraviolet (BUV) reagent is in the cocktail, it is best used within 2 hours after preparation irrespective of storage temperature. Example of creating a 5-Color Fluorescent Antibody Cocktail containing 2 different Brilliant Violet™ Conjugates Final Volume per Test = 90 μL _____________________________________________________________________________________ Total Number of Tests Volume\/Test (μL) 1 3 5 10 Brilliant Stain Buffer 50 50 150 250 500 Reagent 1 (BV) 5 5 15 25 50 Reagent 2 (BV) 5 5 15 25 50 Reagent 3 5 5 15 25 50 Reagent 4 5 5 15 25 50 Reagent 5 20 20 60 100 200 Total Volume 90 90 270 450 900 _____________________________________________________________________________________ Add desired volume of Reagent Cocktail (90 μL in this 5-color example) to all tubes or wells using the protocols for staining human cells described above. Compensation and Setup BD Horizon Brilliant Stain Buffer can be used in single color compensation controls using cells. The buffer is compatible with BD™ Compbeads, however, it has not been tested with compensation beads from other vendors. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. Additionally, the most accurate compensation will be created when BD Horizon Brilliant Stain Buffer is used in all compensation controls, including Brilliant polymer and non-polymer dyes.\u003cbr\u003e\nProduct Notices: Product Notices Alexa Fluor® is a registered trademark of Life Technologies Corporation. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400710825,"sku":"563794","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560835","title":"BD, 560835, BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD3","description":"\u003cp\u003eAlternative Name: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3\/Leu-4 epsilon\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: I WT3; III 471\u003cbr\u003e\nRRID: AB_2033956\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027. This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400776361,"sku":"560835","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563204","title":"BD, 563204, BD Horizon™ BV510 Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_2738067\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349).\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400809129,"sku":"563204","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564997","title":"BD, 564997, BD Horizon™ Fixable Viability Stain 700","description":"\u003cp\u003eApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869637\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS700 dye powder and 310 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. Add 310 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Red laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD LSRFortessa™, or BD LSR™ II) can be used. This dye can be read out of filters commonly used for BD Horizon™ APC-R700 or Alexa Fluor® 700 (eg, 730\/45- or 712\/21-nm filter). Fluorescence compensation is best achieved using stained and unstained samples of the cells of interest. Procedure Fixable Viability Stain 700 labeling of cells 1. Prepare cells for flow cytometric staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1-10 x 10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of BD Horizon™ Fixable Viability Stain 700 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance as different cell types and different applications can result in a wide degree of variability in staining. Please see Note 1 below for guidance on recommended ranges. 5. Incubate the mixture for 10-15 minutes at room temperature or 2-8°C protected from light. a. Optional: Alternatively, incubate mixtures at 37°C for 5-7 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentrations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. The following are ranges that we have found to work for various cell systems: a. Erythrocyte-Lysed Whole Blood Cells: 1:1,000 from the Stock Solution. b. Primary Cells: 1:1,000 - 1:4,000 from the Stock Solution. c. Cell Lines: 1:4,000 - 1:10,000 from the Stock Solution. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (eg, FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 700 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (eg, BD Phosflow™ Perm Buffer III, Cat. No. 558050), intracellular cytokine staining (eg, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Solution Kit, Cat. No. 554714), or transcription factor staining (eg, BD Pharmingen™ Transcription Factor Buffer Set, Cat. No. 562574\/562725). 5. Apoptotic cells can show variable staining. We recommend co-staining with other fluorescent probes such as Annexin V FITC (Cat. No. 556419) if further analysis is desired for the apoptotic cells.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401005737,"sku":"564997","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-559763","title":"BD, 559763, BD Pharmingen™ PE Annexin V Apoptosis Detection Kit I","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869265\u003cbr\u003e\nDescription: Description Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including Phycoerythrin (PE). This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, PE Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. PE Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with PE Annexin V is typically used in conjunction with a vital dye such as 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (7-AAD negative, PE Annexin V positive). Viable cells with intact membranes exclude 7-AAD, wheras the membranes of dead and damaged cells are permeable to 7-AAD. For example, cells that are considered viable are PE Annexin V and 7-AAD negative; cells that are in early apoptosis are PE Annexin V positive and 7-AAD negative; and cells that are in late apoptosis or already dead are are both PE Annexin V and 7-AAD positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both PE Annexin V and 7-AAD. However, when apoptosis is measured over time, cells can be often tracked from PE Annexin V and 7-AAD negative (viable, or no measurable apoptosis), to PE Annexin V positive and 7-AAD negative (early apoptosis, membrane integrity is present) and finally to PE Annexin V and 7-AAD positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both PE Annexin V and 7-AAD positive, in of itself, reveals less information about the process by which the cells underwent their demise.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures PE Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. PE Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining as described in the PE Annexin V Staining Protocol. Investigators should note that PE Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however, have been previously reported (Casiola-Rosen et al . and van Engelend et al.). INDUCTION OF APOPTOSIS BY CAMPTOTHECIN The following protocol is provided as an illustration on how PE Annexin V may be used on a cell line (Jurkat). Materials 1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 µM) to 1 x 10^6 Jurkat cells. 2. Incubate the cells for 4-6 hr at 37°C. 3. Proceed with the PE Annexin V Staining Protocol to measure apoptosis. PE ANNEXIN V STAINING PROTOCOL PE Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment.   Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. 7-Amino-Actinomycin (7-AAD) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude 7-AAD, whereas the membranes of dead and damaged cells are permeable to 7-AAD. Cells that stain positive for PE Annexin V and negative for 7-AAD are undergoing apoptosis. Cells that stain positive for both PE Annexin V and 7-AAD are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both PE Annexin V and 7-AAD are alive and not undergoing measurable apoptosis. Reagents 1. PE Annexin V (component no. 51-65875X):  Use 5 µl per test. 2. 7-Amino-Actinomycin (7-AAD) (component no. 51-68981E) is a convenient, ready-to-use nucleic acid dye.  Use 5 µl per test. 3. 10X Annexin V Binding Buffer (component no. 51-66121E):  0.1 M Hepes\/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2.  For a 1X working solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water. Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells\/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of PE Annexin V and 5 µl 7-AAD. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. Add 400 µl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr. SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY The following controls are used to set up compensation and quadrants: 1.  Unstained cells. 2.  Cells stained with PE Annexin V (no 7-AAD). 3.  Cells stained with 7-AAD (no PE Annexin V). Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with PE Annexin V and\/or PE Annexin V and 7-AAD. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (PE Annexin V positive, 7-AAD negative or PE Annexin V positive, 7-AAD positive). The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for 7-AAD as well as for PE Annexin V. Thus the assay does not distinguish between cells that have already undergone an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with both PE Annexin V and 7-AAD.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 2.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401169577,"sku":"559763","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560274","title":"BD, 560274, BD Pharmingen™ APC-H7 Mouse anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nRRID: AB_1645479\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with APC-H7 under optimum conditions, and unconjugated antibody and APC-H7 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Although BD APC-H7 is engineered to minimize spillover to the APC channel and is more stable and less affected by light, temperature, and formaldehyde-based fixatives, compared to other APC-cyanine tandem dyes, it is still good practice to minimize as much as possible, any light, temperature and fixative exposure when working with all fluorescent conjugates. BD APC-H7 is a tandem conjugate and an analog of APC-Cy7 with the same spectral properties. It has decreased intensity but it is engineered for greater stability and less spillover in the APC channel and consequently offers better performance than APC-Cy7. It has an absorption maximum of approximately 650 nm. When excited by light from a red laser, the APC fluorochrome can transfer energy to the cyanine dye, which then emits at a longer wavelength. The resulting fluorescent emission maximum is approximately 767 nm. BD recommends that a 750-nm longpass filter be used along with a red-sensitive detector such as the Hamamatsu R3896 PMT. As with APC-Cy7 special filters are required when using APC-H7 in conjunction with APC. Note: Although our APC-H7 products demonstrate higher lot-to lot consistency than other APC tandem conjugate products, and every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-H7 conjugate. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401366185,"sku":"560274","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561088","title":"BD, 561088, BD Pharmingen™ FITC Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_394609\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401431721,"sku":"561088","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561109","title":"BD, 561109, BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse CD8a","description":"\u003cp\u003eAlternative Name: Cd8a; CD8 alpha chain; Ly-2; Lyt2; Lyt-2; Ly-35; Ly-B\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse Spleen Cells or Thymocyte Membranes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_394081\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401464489,"sku":"561109","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565250","title":"BD, 565250, BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse CD206","description":"\u003cp\u003eAlternative Name: MMR; MR; Macrophage mannose receptor 1; Mrc1; Mannose receptor, C type 1\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat F344, also known as Fischer, CDF IgG2a\u003cbr\u003e\nImmunogen: Recombinant Mouse CD206 Carbohydrate recognition domains 4-7\/Fc Fusion Protein\u003cbr\u003e\nApplication: Intracellular staining (flow cytometry) (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2739133\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. An isotype control should be used at the same concentration as the antibody of interest.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401595561,"sku":"565250","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-552843","title":"BD, 552843, BD™ CompBeads Anti-Mouse Ig, κ\/Negative Control Compensation Particles Set","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nEntrez Gene ID: 16071\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_10051478\u003cbr\u003e\nDescription: Description The BD™ CompBeads Set Anti-Mouse Ig, κ are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD™ CompBeads Anti-Mouse Ig, κ particles, which bind any mouse κ light chain-bearing immunoglobulin, and the BD™ CompBeads Negative Control, which has no binding capacity. When mixed together with a fluorochrome-conjugated mouse antibody, the BD™ CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Note: BD Horizon™ V500 and AmCyan conjugated reagents can show significant differences in emission spectrum on stained cells and when captured on BD™ CompBeads.  Thus, spillover values for these dyes evaluated with BD™ CompBeads may not provide correct compensation for cells.  Therefore, single stained cellular controls are recommended to set up compensation for AmCyan and BD Horizon™ V500 reagents.  BD Horizon™ V500-C has been modified to minimize these spectral differences and BD™ CompBeads may be used to determine spillover values for RUO antibodies conjugated to BD Horizon™ V500-C. Without affecting compensation function, some lots may profile as a bi-modal histogram, which may be possible due to inherent light scatter and\/or residual aggregation of the compensation particles.  Optimization of instrument voltage or gating conditions may be helpful for improving histogram visualization. This BD™ CompBeads Set has been tested with mouse Ig antibodies conjugated to various fluorochromes and analyzed using a BD FACS brand flow cytometer to ensure specificity and reactivity of the particles. See the specific instructions below on the use of the BD™ CompBeads Set: 1.    Vortex BD™ CompBeads thoroughly before use. 2.    Label a separate 12 x 75 mm sample tube for each flurochrome-conjugated mouse Ig, κ antibody to be used on a given experiment. 3.    Add 100 µl of staining buffer [e.g., BD Pharmingen Stain (FBS), Cat. No. 554656 or BD Pharmingen Stain (BSA), Cat. No. 554657] to each tube. 4.    Add 1 full drop (approximately 60 µl) of the BD™ CompBeads Negative Control and 1 drop of the BD™ CompBeads Anti-Mouse Ig, κ  beads to each tube and vortex. 5.    Add 20 µl of each prediluted antibody stock (diluted to a concentration optimal for staining 10^6 cells) to be tested on a given experiment to the appropriately-labeled tube. (Make sure the antibody is deposited to the bead mixture, then vortex.) 6.    Incubate 15 - 30 minutes at room temperature. Protect from exposure to direct light. 7.    During the incubation of beads and antibody, set the flow cytometer instrument PMT voltage settings using the target tissue for the given experiment (eg, whole blood, splenocytes, etc). If you are unsure, use the BD™ CompBeads Negative Control beads as your negative reference point and proceed. 8.    Following the incubation step (see Step 6 above), add 2 ml staining buffer to each tube and pellet by centrifugation at 200 x g for 10 minutes. 9.    Discard supernatant from each tube by careful vacuum aspiration using a fine-tip Pasteur pipette. 10.  Resuspend bead pellet in each tube by adding 0.5 ml of staining buffer to each tube. Vortex thoroughly. 11.  Run each tube separately on the flow cytometer. Gate on the singlet bead population based on FSC (forward-light scatter) and SSC (side-light scatter) characteristics. 12.  Adjust flow rate to 200 - 300 events per second if possible. 13.  Create a dot plot for the given fluorochrome-conjugated antibody as appropriate [i.e., to set compensation for a fluorescein (FITC)-conjugated antibody, use an FL1 vs. FL2 dot plot]. 14.  Place a quadrant gate such that the negative bead population is in the lower left quadrant and the positive bead population is in the upper or lower right quadrant, and adjust the compensation values until the median fluorescence intensity (MFI) of each population (as shown in the quadrant stats window) is approximately equal (i.e., for FL2 -%FL1, the FL2 MFI of both bead populations should be approximately equal when properly compensated). 15.  Repeat Steps 13 and 14 for other tubes, as necessary. 16.  Proceed to acquiring the actual staining experiment.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Cy is a trademark of Amersham Biosciences Limited. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 6.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401628329,"sku":"552843","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564713","title":"BD, 564713, BD Horizon™ BV510 Mouse Anti-Human CD3","description":"\u003cp\u003eAlternative Name: CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: V 5T-CD03.05\u003cbr\u003e\nRRID: AB_2738909\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Brilliant Violet™ 510 is a trademark of Sirigen. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401792169,"sku":"564713","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565410","title":"BD, 565410, BD Pharmingen™ PE Rat Anti-Mouse F4\/80","description":"\u003cp\u003eAlternative Name: Gpf480; F480; Emr1; Ly71; DD7A5-7; EGF-TM7; TM7LN3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat WI, also known as Wistar (outbred) IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse F4\/80 Recombinant Protein\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2687527\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. An isotype control should be used at the same concentration as the antibody of interest. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401857705,"sku":"565410","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561091","title":"BD, 561091, BD Pharmingen™ APC Rat Anti-Mouse CD4","description":"\u003cp\u003eAlternative Name: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4\/Leu-3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat DA, also known as DA\/HA IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse Thymocytes (BALB\/c)\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_398528\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401890473,"sku":"561091","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560976","title":"BD, 560976, BD Pharmingen™ FITC Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_395874\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401956009,"sku":"560976","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560582","title":"BD, 560582, BD Pharmingen™ PE-Cy™7 Rat Anti-Mouse CD86","description":"\u003cp\u003eAlternative Name: B7-2; Ly-58; Cd28l2; Early T-cell costimulatory molecule 1; ETC1; MB7; CLS1\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse (CBA\/Ca) LPS-activated splenic B Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_1727518\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036). Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027. This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded. PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from PE may be observed. Therefore, we recommend that individual compensation controls be performed for every PE-Cy7 conjugate. PE-Cy7 is optimized for use with a single argon ion laser emitting 488-nm light, and there is no significant overlap between PE-Cy7 and FITC emission spectra. When using dual-laser cytometers, which may directly excite both PE and Cy7, we recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402021545,"sku":"560582","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562126","title":"BD, 562126, BD Horizon™ V450 Mouse Anti-Human CD200","description":"\u003cp\u003eAlternative Name: OX-2; MOX1; MOX2 ; My033\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human CD200 Recombinant Protein\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: VII 70655; IX 40\u003cbr\u003e\nRRID: AB_10895578\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402054313,"sku":"562126","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-555749","title":"BD, 555749, BD Pharmingen™ PE Mouse IgG1, κ Isotype Control","description":"\u003cp\u003eIsotype: Mouse IgG1, κ\u003cbr\u003e\nApplication: Flow cytometry, Isotype control (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nRRID: AB_396091\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402087081,"sku":"555749","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-557803","title":"BD, 557803, BD Pharmingen™ FITC Mouse Anti-NHP CD45","description":"\u003cp\u003eAlternative Name: Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag\u003cbr\u003e\nReactivity: Rhesus, Cynomolgus, Baboon (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Rhesus peripheral whole blood\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nRRID: AB_396879\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402185385,"sku":"557803","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560566","title":"BD, 560566, BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_1645452\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402611369,"sku":"560566","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562752","title":"BD, 562752, BD Horizon™ BV421 Mouse Anti-Human CD56","description":"\u003cp\u003eAlternative Name: NCAM1; NCAM-1; NCAM; Leu-19; Neural cell adhesion molecule 1; NKH1; MSK39\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG2b, κ\u003cbr\u003e\nImmunogen: Immunoaffinity-enriched adult human brain NCAM\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: V NK60\u003cbr\u003e\nRRID: AB_2732054\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Brilliant Violet™ 421 is a trademark of Sirigen. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402644137,"sku":"562752","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-552845","title":"BD, 552845, BD™ CompBeads Anti-Rat and Anti-Hamster Ig κ \/Negative Control Compensation Particles Set","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_10058522\u003cbr\u003e\nDescription: Description The BD™ CompBeads Compensation Particles Anti-Rat\/Hamster Ig, κ Set are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD CompBeads Anti-Rat\/Hamster Ig, κ particles, which bind any rat or hamster κ light chain-bearing immunoglobulin, and the BD CompBeads Negative Control (FBS) Particles, which has no binding capacity. When mixed together with a fluorochrome-conjugated rat or hamster antibody, the BD CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Note: BD Horizon™ V500 and AmCyan conjugated reagents can show significant differences in emission spectrum on stained cells and when captured on BD™ CompBeads.  Thus, spillover values for these dyes evaluated with BD™ CompBeads may not provide correct compensation for cells.  Therefore, single stained cellular controls are recommended to set up compensation for AmCyan and BD Horizon™ V500 reagents.  BD Horizon™ V500-C has been modified to minimize these spectral differences and BD™ CompBeads may be used to determine spillover values for RUO antibodies conjugated to BD Horizon™ V500-C. Without affecting compensation function, some lots may profile as a bi-modal histogram, which may be possible due to inherent light scatter and\/or residual aggregation of the compensation particles.  Optimization of instrument voltage or gating conditions may be helpful for improving histogram visualization. This BD™ CompBeads Set has been tested with hamster Ig antibodies conjugated to various fluorochromes and analyzed using a BD FACS brand flow cytometer to ensure specificity and reactivity of the particles.  See the specific instructions below on the use of the BD™ CompBeads Set: 1.   Vortex BD™ CompBeads thoroughly before use. 2.   Label a separate 12 x 75 mm sample tube for each flurochrome-conjugated mouse Ig, κ antibody to be used on a given experiment. 3.   Add 100 µl of staining buffer [eg, BD Pharmingen Stain (FBS), Cat. No. 554656 or BD Pharmingen Stain (BSA), Cat. No. 554657] to each tube. 4.   Add 1 full drop (approximately 60 µl) of the BD CompBead Negative Control (FBS) and 1 drop of the BD™ CompBead Anti-Rat\/Hamster Ig, κ beads to each tube and vortex. 5.   Add 20 µl of each prediluted antibody stock (diluted to a concentration optimal for staining 10^6 cells) to be tested on a given experiment to the appropriately-labeled tube. (Make sure the antibody is deposited to the bead mixture, then vortex.) 6.   Incubate 15 - 30 minutes at room temperature. Protect from exposure to direct light. 7.   During the incubation of beads and antibody, set the flow cytometer instrument PMT voltage settings using the target tissue for the given experiment (eg, whole blood, splenocytes, etc.). If you are unsure, use the BD CompBeads Negative Control (FBS*) beads as your negative reference point and proceed. 8.   Following the incubation step (see Step 6 above), add 2 ml staining buffer to each tube and pellet by centrifugation at 200 x g for 10 minutes. 9.   Discard supernatant from each tube by careful vacuum aspiration using a fine-tip Pasteur pipette. 10. Resuspend bead pellet in each tube by adding 0.5 ml of staining buffer to each tube. Vortex thoroughly. 11. Run each tube separately on the flow cytometer. Gate on the singlet bead population based on FSC (forward-light scatter) and SSC (sidelight scatter) characteristics. 12. Adjust flow rate to 200 - 300 events per second if possible. 13. Create a dot plot for the given fluorochrome-conjugated antibody as appropriate [i.e., to set compensation for a fluorescein (FITC)-conjugated antibody, use an FL1 vs. FL2 dot plot]. 14. Place a quadrant gate such that the negative bead population is in the lower left quadrant and the positive bead population is in the upper or lower right quadrant, and adjust the compensation values until the median fluorescence intensity (MFI) of each population (as shown in the quadrant stats window) is approximately equal (i.e., for FL2 -%FL1, the FL2 MFI of both bead populations should be approximately equal when properly compensated). 15. Repeat Steps 13 and 14 for other tubes, as necessary. 16. Proceed to acquiring the actual staining experiment.\u003cbr\u003e\nProduct Notices: Product Notices Cy is a trademark of Amersham Biosciences Limited. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http:\/\/www.bdbiosciences.com\/documents\/hamster_chart_11x17.pdf. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 6.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402676905,"sku":"552845","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563891","title":"BD, 563891, BD Horizon™ BV510 Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2734134\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402742441,"sku":"563891","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561005","title":"BD, 561005, BD Pharmingen™ FITC Mouse Anti-Human CD4","description":"\u003cp\u003eReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: PHA-stimulated Human Peripheral Blood Mononuclear Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nWorkshop Number: IV T114; VI 6T-079\u003cbr\u003e\nRRID: AB_395751\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402775209,"sku":"561005","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-550628","title":"BD, 550628, BD Pharmingen™ FITC Mouse Anti-Human CD4","description":"\u003cp\u003eAlternative Name: L3T4 ; T-cell surface antigen T4\/Leu-3; W3\/25 ; CD4 antigen (p55)\u003cbr\u003e\nReactivity: Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human HPB-ALL Cell Line\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nRRID: AB_393789\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402807977,"sku":"550628","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565967","title":"BD, 565967, BD Horizon™ BUV395 Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2651134\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402840745,"sku":"565967","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562659","title":"BD, 562659, BD Horizon™ BV605 Mouse Anti-Human CD4","description":"\u003cp\u003eAlternative Name: L3T4; T-cell surface antigen T4\/Leu-3; W3\/25; CD4 antigen (p55)\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: PHA-stimulated Human Peripheral Blood Mononuclear Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV T114; VI 6T-079\u003cbr\u003e\nEntrez Gene ID: 920\u003cbr\u003e\nRRID: AB_2744420\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https:\/\/www.bdbiosciences.com\/en-us\/support\/product-notices. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). For U.S. patents that may apply, see bd.com\/patents.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402906281,"sku":"562659","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554061","title":"BD, 554061, BD Pharmingen™ PE Streptavidin","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_10053328\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802402939049,"sku":"554061","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560987","title":"BD, 560987, BD Pharmingen™ APC Mouse Anti-Human CD25","description":"\u003cp\u003eAlternative Name: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Phytohemagglutinin stimulated human lymphocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 20 µl\u003cbr\u003e\nWorkshop Number: IV A053\u003cbr\u003e\nRRID: AB_398598\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403004585,"sku":"560987","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-566385","title":"BD, 566385, BD Horizon™ Brilliant Stain Buffer Plus","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Protocols for Multicolor Immunofluorescent Staining of Cells Using BD Horizon Brilliant Stain Buffer Plus Multicolor Surface Staining of Human Cell Samples in Tubes or 96-Well Plates Using Individual Staining Reagents 1. Add 10 μL of BD Horizon Brilliant Stain Buffer Plus to all tubes or desired wells for the experiment Note: The 10 μL amount of Brilliant Stain Buffer Plus per tube or per well does not depend on the final staining volume or amount of cells used per tube or number of BD fluorescent antibodies used in staining.  Although written for use with human cells, these protocols can readily be adapted for analyzing mouse cells or cells from other species, for example, by staining mouse cells at 4°C rather than at room temperature (RT). 2. Add each fluorescent reagent at the recommended volume per test (eg, 5 μL or 20 μL). Proceed to Protocol 1, 2, or 3. Note: If total volume of mixture from steps 1 and 2 does not meet or exceed 50 μL  per test, bring total volume up to 50 uL by adding BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Protocol 1 for Staining Whole Blood Samples in Tubes a. Add 100 μL of human whole blood to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 mL of BD FACS™ Lysing Solution (Cat. No. 349202; 10 min) or BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899; 15 min) per tube and incubate protected from light at RT e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer, eg, BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or BD Pharmingen™ Stain Buffer (BSA) (Cat. No. 554657) g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 2 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in Tubes a. Add 100 μL of human cells to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 ml of stain\/wash buffer per tube e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 3 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in 96-well Plates Note:    Although written for use with human cells, this 96-well plate-based protocol can readily be adapted for analyzing mouse cells or cells from other species. a. Add 50 μL of human cells to each well b. Incubate (30 min) protected from light at RT c. Wash by adding 100 μL of stain\/wash buffer d. Pellet cells by centrifugation (5 min) at 1400-1500 rpm e. Aspirate supernatant f. Resuspend pelleted cells by adding 250 μL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate supernatant i. Resuspend pelleted cells thoroughly with 150 μL stain\/wash buffer by pipetting the suspended cells several times j. Transfer well contents to tubes and add additional stain\/wash buffer to the tubes as desired for flow cytometric analysis Note: Alternatively, acquire samples for flow cytometric analysis from the plate directly. Multicolor Intracellular Staining of Cell Samples in Tubes or 96-Well Plates Using Individual Staining Reagents 1. Add 10 μL of BD Horizon Brilliant Stain Buffer Plus to all tubes or desired wells for the experiment. Note: The 10 μL amount of Brilliant Stain Buffer Plus per tube or per well does not depend on the final staining volume or amount of cells used per tube or number of BD fluorescent antibodies used in staining. 2. Add each fluorescent reagent at the recommended volume per test (eg, 5 μL or 20 μL). Note: If total volume of mixture from steps 1 and 2 does not meet or exceed 50 uL per test, bring total volume up to 50 uL by adding BD Pharmingen Stain Buffer (FBS). For intracellular staining protocols that must keep cells permeabalized during staining use BD Perm\/Wash™ Buffer (Cat. No. 554723). Protocol 4 for Staining Intracellular targets in either tubes or 96 well plates Note:   For primary surface staining follow protocol 1-3 above as applicable.  If the intracellular protocol requires the use of BD Perm\/Wash Buffer during the staining and wash steps, use BD Perm\/Wash Buffer in steps b, e and h, otherwise use BD Pharmingen Stain Buffer (FBS). a. Permeabilize cells according to desired permeabilization protocol b. Add 50 uL of appropriate staining buffer c. Add one test volume of the mixture from steps 1 and 2 (staining reagents plus BD Horizon Brilliant Stain Buffer Plus) d. Incubate at 4°C for 30-60 minutes in the dark. e. Wash with appropriate  wash buffer (1 ml\/wash for staining in tubes and 250 µl\/wash final volume for staining in microwell plates) f. Pellet cells by centrifugation (5 min) at 1400-1500 rpm g. Aspirate supernatant h. Wash with appropriate  wash buffer (1 ml\/wash for staining in tubes and 250 µl\/wash final volume for staining in microwell plates) i. Pellet cells by centrifugation (5 min) at 1400-1500 rpm j.    Resuspend in BD Pharmingen Stain Buffer (FBS) prior to flow cytometric analysis. Multicolor Staining of Human Cell Samples in Tubes or 96-Well Plates Using Cocktailed Staining Reagents Instead of adding staining reagents individually to each tube or well of a 96-well plate, it may be desirable to add cocktailed staining reagents, ie, mixtures of two or more fluorescent staining reagents. The following protocol provides an example of how to prepare a \"per test\" 5-Color Fluorescent Antibody Cocktail that already contains BD Horizon Brilliant Stain Buffer Plus. Human Samples:  Pre-mixed Fluorescent Reagent Cocktails For each multicolor test of cocktailed fluorescent reagents: i)  Add 10 μL of BD Horizon Brilliant Stain Buffer Plus per test ii)  Add each fluorescent reagent at the recommended volume per test (5 μL or 20 μL) iii)  Add BD Pharmingen Stain Buffer (FBS) (Cat. No. 554656) to bring the volume of each test to a minimum of 50 μL iv)  Mix reagents (especially after adding BD Horizon Brilliant reagents) v)  Store cocktail at 4°C protected from light if it is to be used later Note: Protected from light, fluorescent reagent cocktails containing more than one Brilliant Violet and\/or Brilliant Blue reagent are best used within 24 hours after preparation when stored at 4ºC or within 4 hours when stored at room temperature. However, when more than one Brilliant Ultraviolet (BUV) reagent is in the cocktail, it is best used within 2 hours after preparation irrespective of storage temperature. For additional guidance, see Table 1 \"Example of creating a 5-Color Fluorescent Antibody Cocktail containing 2 different BD Horizon™ Brilliant Violet (BV) Conjugates\" at the end of this field. Compensation and Setup BD Horizon™  Brilliant Stain Buffer Plus can be used in single color compensation controls using cells. The buffer is compatible with BD® Compbeads, however, it has not been tested with compensation beads from other vendors. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon™ Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. Additionally, the most accurate compensation will be created when BD Horizon™ Brilliant Stain Buffer is used in all compensation controls, including Brilliant polymer and non-polymer dyes.\u003cbr\u003e\nProduct Notices: Product Notices Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Alexa Fluor® is a registered trademark of Life Technologies Corporation. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403037353,"sku":"566385","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563101","title":"BD, 563101, BD Pharmingen™ PE Rat anti-Mouse Foxp3","description":"\u003cp\u003eAlternative Name: Forkhead box P3; IPEX; Forkhead box protein P3; JM2; Scurfin; Scurfy; Sf\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nImmunogen: Foxp3 Recombinant Protein\u003cbr\u003e\nApplication: Intracellular staining (flow cytometry) (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2738006\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403070121,"sku":"563101","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563024","title":"BD, 563024, BD Horizon™ BV510 Hamster Anti-Mouse CD3e","description":"\u003cp\u003eAlternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Armenian Hamster IgG1, κ\u003cbr\u003e\nImmunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2737959\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Brilliant Violet™ 510 is a trademark of Sirigen. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http:\/\/www.bdbiosciences.com\/documents\/hamster_chart_11x17.pdf.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403102889,"sku":"563024","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560650","title":"BD, 560650, BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD4","description":"\u003cp\u003eReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: PHA-stimulated Human Peripheral Blood Mononuclear Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV T114; VI 6T-079\u003cbr\u003e\nRRID: AB_1727476\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027. This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403135657,"sku":"560650","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561108","title":"BD, 561108, BD Pharmingen™ PerCP-Cy™5.5 Hamster Anti-Mouse CD3e","description":"\u003cp\u003eAlternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Armenian Hamster IgG1, κ\u003cbr\u003e\nImmunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_394082\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http:\/\/www.bdbiosciences.com\/documents\/hamster_chart_11x17.pdf. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403201193,"sku":"561108","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561194","title":"BD, 561194, BD Pharmingen™ PerCP-Cy™5.5 Mouse anti-Human CD64","description":"\u003cp\u003eAlternative Name: FCGR1; FcRI; Fc-gamma RI; IgG Fc Receptor I; High affinity IgG FcR1\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human Rheumatoid synovial fluid cells and fibronectin-purified monocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: VI MA36\u003cbr\u003e\nRRID: AB_10563216\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403299497,"sku":"561194","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562843","title":"BD, 562843, BD Horizon™ BV605 Mouse Anti-Human CD4","description":"\u003cp\u003eAlternative Name: L3T4 ; T-cell surface antigen T4\/Leu-3; W3\/25 ; CD4 antigen (p55)\u003cbr\u003e\nReactivity: Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human HPB-ALL Cell Line\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nRRID: AB_2737833\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV605 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV605 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD Optibuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate. CF™ is a trademark of Biotium, Inc. BD Horizon Brilliant Violet 605 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403332265,"sku":"562843","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-557757","title":"BD, 557757, BD Pharmingen™ APC-Cy™7 Mouse Anti-Human CD3","description":"\u003cp\u003eAlternative Name: CD3E; CD3-epsilon; T3E; TCRE\u003cbr\u003e\nReactivity: Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, λ\u003cbr\u003e\nImmunogen: Purified Human CD3ε Protein\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested), Induction (Reported)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nRRID: AB_396863\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with APC-Cy7 under optimum conditions, and unconjugated antibody and free APC-Cy7 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, thus maximizing its fluorescence emission intensity, minimizing residual emission from APC, and minimizing required electronic compensation in multilaser-laser flow cytometry systems. Note: Although every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-Cy7 conjugate. APC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036). Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403365033,"sku":"557757","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561114","title":"BD, 561114, BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-CD11b","description":"\u003cp\u003eAlternative Name: Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain\u003cbr\u003e\nReactivity: Mouse (QC Testing), Human (Tested in Development)\u003cbr\u003e\nIsotype: Rat DA, also known as DA\/HA IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Splenic Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_394002\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of GE Healthcare. Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403397801,"sku":"561114","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564500","title":"BD, 564500, BD Horizon™ BB515 Mouse Anti-Human CD4","description":"\u003cp\u003eAlternative Name: L3T4 ; T-cell surface antigen T4\/Leu-3; W3\/25 ; CD4 antigen (p55)\u003cbr\u003e\nReactivity: Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human HPB-ALL Cell Line\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nRRID: AB_2738828\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BB515 under optimum conditions and unconjugated antibody was removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescence staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Species cross-reactivity detected in product development may not have been confirmed on every format and\/or application. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403463337,"sku":"564500","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562971","title":"BD, 562971, BD Horizon™ BV510 Mouse Anti-Human CD4","description":"\u003cp\u003eAlternative Name: L3T4; Leu3a; T-cell surface antigen T4\/Leu-3 ; W3\/25 ; CD4 antigen (p55)\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood T Cells\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: I T38; III T140,496\u003cbr\u003e\nRRID: AB_2744424\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Brilliant Violet™ 510 is a trademark of Sirigen. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403528873,"sku":"562971","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562600","title":"BD, 562600, BD Horizon™ BV421 Hamster Anti-Mouse CD3e","description":"\u003cp\u003eAlternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Armenian Hamster IgG1, κ\u003cbr\u003e\nImmunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_11153670\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. Brilliant Violet™ 421 is a trademark of Sirigen. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http:\/\/www.bdbiosciences.com\/documents\/hamster_chart_11x17.pdf.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403561641,"sku":"562600","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563151","title":"BD, 563151, BD Horizon™ BV605 Rat Anti-Mouse CD4","description":"\u003cp\u003eAlternative Name: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4\/Leu-3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat DA, also known as DA\/HA IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse Thymocytes (BALB\/c)\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2687549\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV605 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV605 were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. CF™ is a trademark of Biotium, Inc.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403594409,"sku":"563151","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562438","title":"BD, 562438, BD Horizon™ BV421 Mouse IgG1, k Isotype Control","description":"\u003cp\u003eAlternative Name: IgG1, kappa Isotype Control (Anti-KLH)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Keyhole limpet hemocyanin (KLH)\u003cbr\u003e\nApplication: Flow cytometry, Isotype control (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_11207319\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant™ Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant™ Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant™ Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794\/566349) or the BD Horizon Brilliant™ Stain Buffer Plus (Cat. No. 566385).\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Pacific Blue™ is a trademark of Life Technologies Corporation.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802403627177,"sku":"562438","price":0.99,"currency_code":"USD","in_stock":true}]}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/collections\/bd-cytofic-cytoperm-kit.webp?v=1761808260","url":"https:\/\/iright.com\/collections\/bd-flow-cytometry-reagents.oembed?page=114","provider":"Iright","version":"1.0","type":"link"}