{"title":"BD Reagents \u0026 Tools: Flow Cytometry, Single-Cell, Imaging, Immunoassay \u0026 More","description":"\u003cp\u003eBD delivers validated reagents and tools that power discovery from sample prep to readout—across flow cytometry, single-cell multiomics, microscopy imaging, immunoassays, Western blotting\/molecular workflows, and functional cell assays. Use this primary hub to explore product families, compare use cases side-by-side, and jump into focused collections with technical guidance and support.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-bioscience-image.jpg?v=1762525391\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch2\u003eBD Product Families Overview\u003c\/h2\u003e\n\u003cp\u003eFrom screening to deep characterization, BD product families map to the platforms you already run and the answers you need. Start with the application that matches your assay goals, then follow through to the dedicated collection page for specifications, popular SKUs, protocols, and compatibility notes.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-flow-cytometry-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eFlow Cytometry Reagents\u003c\/strong\u003e\u003c\/a\u003e — Antibodies, buffers, dyes, and controls for high-parameter analysis and sorting.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-cytofic-cytoperm-kit.webp?v=1761808246\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-pdp-single-cell-multiomics-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003ePDP Single Cell Multiomics Reagents\u003c\/strong\u003e\u003c\/a\u003e — Library prep and barcoding chemistries for multi-omic single-cell profiling.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-single-cell-multiplexing-kits.avif?v=1762329211\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-microscopy-imaging-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eMicroscopy Imaging Reagents\u003c\/strong\u003e\u003c\/a\u003e — Fluorophores, probes, counterstains, and mounting media for fixed and live-cell imaging.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-horizon-brilliant-stain-buffer.avif?v=1762332786\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-pdp-immunoassay-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003ePDP Immunoassay Reagents\u003c\/strong\u003e\u003c\/a\u003e — Beads, capture\/detection systems, and assay buffers for quantitative protein measurements.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-cytometric-bead-array.avif?v=1762343095\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-western-blotting-and-molecular-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eWestern Blotting and Molecular Reagents\u003c\/strong\u003e\u003c\/a\u003e — Membranes, blocking buffers, ECL substrates, antibodies, and nucleic-acid tools.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-pharmingen-hrp-goat-anti-mouse.avif?v=1762403629\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-cell-preparation-separation-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eCell Preparation and Separation Reagents\u003c\/strong\u003e\u003c\/a\u003e — RBC lysis, density media, selection kits, and cleanup solutions for clean inputs.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-cpt-pbmc-idolation.avif?v=1762407299\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/iright.com\/collections\/bd-functional-cell-based-reagents\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eFunctional Cell-Based Reagents\u003c\/strong\u003e\u003c\/a\u003e — Stimulation, activation, differentiation, and viability tools for mechanism and response studies.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/bd-fitc-annexin-v-apoptosis-detection-kit.avif?v=1762412746\" alt=\"\"\u003e\u003c\/p\u003e\n\u003ch2\u003eHow to Choose the Right BD Reagents\u003c\/h2\u003e\n\u003cp\u003eSelecting the optimal set starts with your biological question, the platform you’ll run, and downstream readouts. Use the quick framework below to narrow options, confirm instrument and buffer compatibility, and plan controls to support confident interpretation and reproducible results.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBy platform:\u003c\/strong\u003e Flow cytometry for deep phenotyping and sorting; microscopy for spatial context; immunoassay for quantitation; Western blot\/molecular for target confirmation; single-cell for multi-omic depth.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBy objective:\u003c\/strong\u003e Discovery screens vs. verified quantitation; endpoint imaging vs. live-cell kinetics; rare-cell isolation vs. bulk prep.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Panel design, fluor overlap, filter sets, fixation\/permeabilization chemistry, species reactivity, and sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eControls \u0026amp; QC:\u003c\/strong\u003e Isotypes, FMO, loading controls, standard curves, internal standards, and validated reference materials.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eThroughput \u0026amp; scale:\u003c\/strong\u003e Plate vs. tube, multiplexing level, automation readiness, and sample turnaround targets.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompliance \u0026amp; records:\u003c\/strong\u003e SDS\/IFU availability, certificates, and traceability for regulated environments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSide-by-Side Comparison\u003c\/h2\u003e\n\u003cp\u003eThis table provides a fast, at-a-glance view across BD families so you can move from idea to the most relevant collection page in one step. For SKU-level details, protocols, and compatibility notes, continue on to the linked collections.\u003c\/p\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eFamily\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003ePrimary Use\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eTypical Applications\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eCompatibility \/ Readout\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eHighlights\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFlow Cytometry Reagents\u003c\/td\u003e\n\u003ctd\u003eHigh-parameter cell analysis and sorting\u003c\/td\u003e\n\u003ctd\u003eImmune profiling, rare-cell detection, viability, functional readouts\u003c\/td\u003e\n\u003ctd\u003eCytometers\/sorters; fluorescence readout\u003c\/td\u003e\n\u003ctd\u003eBroad fluor panel, validated antibodies, buffers \u0026amp; controls\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePDP Single Cell Multiomics Reagents\u003c\/td\u003e\n\u003ctd\u003eSingle-cell, multi-omic library prep\u003c\/td\u003e\n\u003ctd\u003eTranscript\/protein co-profiling, heterogeneity mapping\u003c\/td\u003e\n\u003ctd\u003eSingle-cell instruments \u0026amp; NGS\u003c\/td\u003e\n\u003ctd\u003eScalable barcoding, robust chemistry, sample-type flexibility\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMicroscopy Imaging Reagents\u003c\/td\u003e\n\u003ctd\u003eFixed\/live-cell imaging and staining\u003c\/td\u003e\n\u003ctd\u003eLocalization, co-staining, morphology, time-lapse\u003c\/td\u003e\n\u003ctd\u003eWidefield\/confocal; fluorescence readout\u003c\/td\u003e\n\u003ctd\u003eBright, photostable probes; clean backgrounds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePDP Immunoassay Reagents\u003c\/td\u003e\n\u003ctd\u003eProtein quantitation and multiplex\u003c\/td\u003e\n\u003ctd\u003eBiomarker panels, cytokine profiling\u003c\/td\u003e\n\u003ctd\u003ePlate\/flow bead platforms; absorbance\/fluorescence\u003c\/td\u003e\n\u003ctd\u003eSensitive, reproducible, multiplex-ready\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWestern Blotting \u0026amp; Molecular Reagents\u003c\/td\u003e\n\u003ctd\u003eTarget detection\/confirmation\u003c\/td\u003e\n\u003ctd\u003eProtein validation, pathway analysis, DNA\/RNA tools\u003c\/td\u003e\n\u003ctd\u003eGel\/western systems; chemiluminescence\u003c\/td\u003e\n\u003ctd\u003eConsistent membranes, ECL, validated antibodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCell Prep \u0026amp; Separation Reagents\u003c\/td\u003e\n\u003ctd\u003eClean input and subset enrichment\u003c\/td\u003e\n\u003ctd\u003ePBMC prep, RBC lysis, positive\/negative selection\u003c\/td\u003e\n\u003ctd\u003eCentrifuge, magnets, density media\u003c\/td\u003e\n\u003ctd\u003eHigh recovery, gentle processing, reproducibility\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFunctional Cell-Based Reagents\u003c\/td\u003e\n\u003ctd\u003ePerturbation and response assays\u003c\/td\u003e\n\u003ctd\u003eActivation, stimulation, viability\/tox, differentiation\u003c\/td\u003e\n\u003ctd\u003ePlate readers, imagers, cytometers\u003c\/td\u003e\n\u003ctd\u003eDefined stimuli, robust controls, clear endpoints\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cp\u003eThese quick answers address common planning and validation topics. For panel design, protocol tuning, or equivalency checks, jump into the relevant collection or contact support for a fast review.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1. How do I reconcile fluor overlap across multi-color panels?\u003c\/strong\u003e\u003cbr\u003eStart with instrument filter sets, pick non-overlapping backbones for critical markers, add spillover controls, and verify with FMOs before scaling.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. What’s the best way to confirm target identity across platforms?\u003c\/strong\u003e\u003cbr\u003eUse orthogonal validation: immunoassay for quantitation, Western blot for size\/isoform confirmation, and imaging for spatial context.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Can I mix fixation\/permeabilization chemistries across steps?\u003c\/strong\u003e\u003cbr\u003eKeep chemistries consistent with antibody\/probe recommendations; mismatches can reduce epitope accessibility or alter fluorescence.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4. How do I approach single-cell experiments with limited input?\u003c\/strong\u003e\u003cbr\u003ePrioritize gentle prep and cleanup, validate recovery with pilot runs, and choose chemistries rated for low-input performance.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5. What documents support audits and training?\u003c\/strong\u003e\u003cbr\u003eSDS\/IFU, data sheets, and lot certificates are available; maintain copies in your ELN\/LIMS with protocol versions and lot numbers.\u003c\/p\u003e\n\u003cdiv class=\"product-full-width\"\u003e\n\u003cdiv class=\"product-block\"\u003e\n\u003ch3\u003eOrder Guidelines\u003c\/h3\u003e\n\u003cp\u003e1. Price \u0026amp; Stock Available on Request. \u003cb\u003e\u003ca href=\"mailto:service@iright.com\"\u003eClick to send email to: service@iright.com\u003c\/a\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e2. Please DO NOT make any payment before confirmation.\u003c\/p\u003e\n\u003cp\u003e3. Minimum order value of $1,000 USD required.\u003c\/p\u003e\n\u003cp\u003e4. 100% prepayment required.\u003c\/p\u003e\n\u003ch3\u003eCollaboration\u003c\/h3\u003e\n\u003cp\u003eTony Tang\u003c\/p\u003e\n\u003cp\u003eEmail: Tony.Tang@iright.com\u003c\/p\u003e\n\u003cp\u003eMobile\/WhatsApp\/Wechat: +86-17717886924\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","products":[{"product_id":"bd-555899","title":"BD, 555899, BD Pharm Lyse™ Lysing Buffer","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C. 1X diluted solutions may be stored at 4° C for up to 30 days.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation of 1X lysing solution: Dilute the 10X concentrate 1:10 with distilled water. The pH of the 1X solution should fall within the range of pH 7.1-7.4.  Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples. Lysing procedure: Note: The following procedure is only applied to human whole blood red blood cell lysis. Since applications vary, for other samples such as mouse spleen red blood cell lysis (lysing incubation time up to 3 minutes at 37°C), bone marrow red blood cell lysis, each investigator should optimize the condition to obtain optimal results. 1. Add 2.0 ml  of 1X lysing solution to each tube containing up to 200 µl of a whole blood plus monoclonal antibody mixture. 2. Gently vortex each tube immediately after adding the lysing solution. 3. Incubate at room temperature, protected from light, for 15 minutes. 4. Centrifuge 200 X g for 5 minutes. 5. Carefully aspirate supernatent, without disturbing pellet. 6. Add 2.0 ml 1X PBS containing 1% heat-inactivated fetal bovine serum and 0.1% sodium azide (PBS-FBS). 7. Centrifuge at 200 X g for 5 minutes. 8. Carefully aspirate supernatent, without disturbing pellet. 9. Resuspend pellet in 0.5 ml PBS-FBS or a fixative such as 2% formaldehyde for flow cytometric analysis. Troubleshooting: Incomplete lysis may occur for several reasons: (1) The age of the specimen may affect red cell lysis, (2) The 1X lysing solution may not have been warmed to room temperature, or (3) After adding the lysing solution, the samples may not have been vortexed sufficiently. If incomplete lysis occurs (recognized by the presence of an excessive amount of visible red blood cells in the final, washed cell suspension), repeat lysing procedure. However, lysing more than two times is not recommended.\u003cbr\u003e\nProduct Notices: Product Notices Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400186537,"sku":"555899","price":0.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/BD-555899.webp?v=1768806209"},{"product_id":"bd-553141","title":"BD, 553141, BD Pharmingen™ Purified Rat Anti-Mouse CD16\/CD32 (Mouse BD Fc Block™)","description":"\u003cp\u003eAlternative Name: FcγRIII\/FcγRII; Fcgr3\/Fcgr2\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse BALB\/c Macrophage J774\u003cbr\u003e\nApplication: Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_394656\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg\/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent. To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis: 1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16\/CD32 mAb 2.4G2 (eg, ≤ 1 μg\/million cells in 100 μl) at 4˚C for 5 minutes. 2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells). 3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG 2b , κ). For additional information on using Mouse BD Fc Block™, refer to the \"Reducing non-specific staining with Fc Block\" section of our Flow Cytometry protocols at our website: https:\/\/www.bdbiosciences.com\/en-us\/resources\/protocols\/flow-cytometry\u003cbr\u003e\nProduct Notices: Product Notices Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA\/LE (No Azide\/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400219305,"sku":"553141","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554714","title":"BD, 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Kit","description":"\u003cp\u003eRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869008\u003cbr\u003e\nDescription: Description This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation\/permeabilization solution and BD Perm\/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm\/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 125.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400252073,"sku":"554714","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-556547","title":"BD, 556547, BD Pharmingen™ FITC Annexin V Apoptosis Detection Kit I","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869082\u003cbr\u003e\nDescription: Description Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V positive). Viable cells with intact membranes exclude PI, wheras the membranes of dead and damaged cells are permeable to PI. For example, cells that are considered viable are FITC Annexin V and PI negative; cells that are in early apoptosis are FITC Annexin V positive and PI negative; and cells that are in late apoptosis or already dead are are both FITC Annexin V and PI positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both FITC Annexin V and PI. However, when apoptosis is measured over time, cells can be often tracked from FITC Annexin V and PI negative (viable, or no measurable apoptosis), to FITC Annexin V positive and PI negative (early apoptosis, membrane integrity is present) and finally to FITC Annexin V and PI positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both FITC Annexin V and PI positive, in of itself, reveals less information about the process by which the cells underwent their demise.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining as described in the FITC Annexin V Staining Protocol. Investigators should note that FITC Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however, have been previously reported (Casiola-Rosen et al . and van Engelend et al.). INDUCTION OF APOPTOSIS BY CAMPTOTHECIN The following protocol is provided as an illustration on how FITC Annexin V may be used on a cell line (Jurkat). Materials 1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 µM) to 1 x 10^6 Jurkat cells. 2. Incubate the cells for 4-6 hr at 37°C. 3. Proceed with the FITC Annexin V Staining Protocol to measure apoptosis. FITC ANNEXIN V STAINING PROTOCOL FITC Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment.   Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Propidium Iodide (PI) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Cells that stain positive for FITC Annexin V and negative for PI are undergoing apoptosis. Cells that stain positive for both FITC Annexin V and PI are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both FITC Annexin V and PI are alive and not undergoing measurable apoptosis. Reagents 1. FITC Annexin V (component no. 51-65874X):  Use 5 µl per test. 2. Propidium Iodide (PI) (component no. 51-66211E) is a convenient, ready-to-use nucleic acid dye.  Use 5 µl per test. 3. 10X Annexin V Binding Buffer (component no. 51-66121E):  0.1 M Hepes\/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2.  For a 1X working solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water. Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells\/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. Add 400 µl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr. SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY The following controls are used to set up compensation and quadrants: 1.  Unstained cells. 2.  Cells stained with FITC Annexin V (no PI). 3.  Cells stained with PI (no FITC Annexin V). Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with FITC Annexin V and\/or FITC Annexin V and PI. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (FITC Annexin V positive, PI negative or FITC Annexin V positive, PI positive). The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for FITC Annexin V. Thus the assay does not distinguish between cells that have already undergone an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with both FITC Annexin V and PI.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 2.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400284841,"sku":"556547","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564219","title":"BD, 564219, BD Pharmingen™ Human BD Fc Block™","description":"\u003cp\u003eReactivity: Human (QC Testing), Rhesus (Tested in Development)\u003cbr\u003e\nIsotype: Human IgG1\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_2728082\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Incubate 1 million cells suspended in 50-100 µL of staining buffer with 2.5 µg of Human BD Fc Block™ (10 minutes at room temperature) followed by staining with the desired fluorescent antibody. No washing step is needed between the blocking and staining steps.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA\/LE (No Azide\/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400317609,"sku":"564219","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554656","title":"BD, 554656, BD Pharmingen™ Stain Buffer (FBS)","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Flow Cytometry (Direct immunofluorescence staining): 1.   Prepare single-cell suspensions from either lymphoid tissue, bone marrow, peripheral blood or cell cultures using standard protocols. 2.   Wash the cells twice in cold Stain Buffer (FBS) and pellet the cells by centrifugation (e.g., 300 x g at 4°C). Resuspend the cell pellet with cold Stain Buffer (FBS) to a final concentration of 2 x 10e7 cells\/ml. 3.   Distribute 50 µl aliquots of the cell suspension (10e6 cells) to either tubes or the round-bottomed wells of microwell plates. 4.   Dilute fluorescent antibodies to their predetermined optimal concentrations in Stain Buffer (FBS) and add small aliquots (e.g., 10 µl) of the diluted antibodies to the tubes or microwells that contain the target cell suspensions.  Incubate for 20 minutes on ice protected from light. Staining time may be increased (≥ 45 min) depending on the avidity of the fluorescent antibody. 5.   Wash the cells two times with either 200 µl (for microwell plates) or 1 ml (for tubes) volumes of Stain Buffer (FBS) to remove unbound antibodies. Centrifuge cells as 300 x g for 5 min. After each centrifugation, carefully aspirate (for microwell plates or tubes) or invert and blot away (for tubes) supernatants from cell pellets. 6.   Resuspend the cell pellet in either 200 µl (for microwell plates) or 0.5 ml (for tubes) volumes of Stain Buffer (FBS). Transfer stained cells from microwell plates to the appropriate tubes for flow cytometric analysis (adjust final volume to ~0.5 ml). 7.   Analyze stained cell samples by flow cytometry as soon as possible (e.g., ≤ 4 hours) after staining. If analysis must be delayed, then the stained cells can be fixed with buffered paraformaldehyde (e.g., BD Cytofix™ Fixation Buffer; Cat. No. 554655) and stored at 4°C (protected from light). The fixed cells should be analyzed as soon as possible (e.g., up to one week after staining and fixation). Note 1: Stain Buffer (FBS) can similarly be used for the indirect immunofluorescent staining of cells. In this case, repeat steps 4 and 5 when using either unlabeled or biotinylated primary antibodies. When staining cells with biotinylated antibodies and fluorochrome-conjugated avidin, it is desirable to use a staining medium that contains no biotin, such as Stain Buffer (BSA) (Cat. No. 554657). Note 2: Stain Buffer (FBS) can also be used for the immunofluorescent staining of surface antigens expressed by cells that are destined to be fixed and immunofluorescently stained for intracellular antigens such as cytokines. Cells stained for intracellular cytokines can be resuspended and maintained (i.e., at 4°C, protected from light) in Stain Buffer (FBS) prior to analysis by flow cytometry.\u003cbr\u003e\nProduct Notices: Product Notices Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400350377,"sku":"554656","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-559925","title":"BD, 559925, BD Pharmingen™ 7-AAD","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400415913,"sku":"559925","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-550583","title":"BD, 550583, BD Pharmingen™ Leukocyte Activation Cocktail, with BD GolgiPlug™, 2x100uL","description":"\u003cp\u003eBD, 550583, BD Pharmingen™ Leukocyte Activation Cocktail, with BD GolgiPlug™, 2x100uL\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400448681,"sku":"550583","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560779","title":"BD, 560779, BD Horizon™ V500 Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_1937324\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V500 under optimum conditions, and unreacted BD Horizon™ V500 was removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. BD Horizon V500 has a maximum absorption of 415 nm and maximum emission of 500 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon V500 is covered by one or more of the following US patents: 8,431,416. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400481449,"sku":"560779","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565388","title":"BD, 565388, BD Horizon™ Fixable Viability Stain 780","description":"\u003cp\u003eApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869673\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS780 dye powder and 180 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. Add 180 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Red laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD™ LSR II, BD LSRFortessa™, or BD Accuri™ C6) can be used. This dye can be read out of filters commonly used for APC-Cy™7 (eg, 780\/60). Fluorescence compensation is best achieved using cell samples of interest. When designing multicolor staining panels, please be aware of fluorescence spillover into the BD Horizon™ BUV737, BD Horizon™ BV786, and PE-Cy™7 (when read off the yellow-green laser) channels. Panels should be optimized to take this spillover into account. To reduce fluorescence spillover, we recommend titrating FVS780 and using the lowest possible dye concentration that provides adequate resolution of live and dead cell populations of interest. Procedure Fixable Viability Stain 780 labeling of cells 1. Prepare cells for flow cytometric staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1-10x10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of BD Horizon™ Fixable Viability Stain 780 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance, as different cell types and different applications can result in a wide degree of variability in staining. 5. Incubate the mixture for 10-15 minutes at room temperature protected from light. a. Optional: Alternatively, incubate mixtures at 37°C for 5-7 minutes or 2-8°C for 30-60 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentrations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (eg, FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 780 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (eg, Cat. No. 558050, BD Phosflow™ Perm Buffer III), intracellular cytokine staining (eg, Cat. No. 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Kit), or transcription factor staining (eg, Cat. No. 562574\/562725, BD Pharmingen™ Transcription Factor Buffer Set). 5. Apoptotic cells can show variable staining. We recommend co-staining with, eg, Annexin V FITC (Cat. No. 556419) if further analysis is desired for the apoptotic cells. Danger: Causes serious eye damage. Precuationary statements: Wear protective gloves\/protective clothing\/eye protection\/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CONTROL\/doctor. Dispose of contents\/containers in an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Cy is a trademark of GE Healthcare. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400514217,"sku":"565388","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558269","title":"BD, 558269, BD™ Cytometric Bead Array (CBA) Human IFN-γ Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869127\u003cbr\u003e\nDescription: Description The BD™ CBA Human IFN-γ Flex Set is a bead-based immunoassay capable of measuring human interferon-γ (IFN-γ) in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IFN-γ Flex Set. The biology and function of IFN-γ has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IFN-γ Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IFN-γ Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IFN-γ Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human IFN-γ Flex Set B8 560111 The BD CBA Human IFN-γ Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 1.8 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400546985,"sku":"558269","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564406","title":"BD, 564406, BD Horizon™ Fixable Viability Stain 510","description":"\u003cp\u003eReactivity: Human, Mouse (Tested in Development)\u003cbr\u003e\nApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869572\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS510 dye powder and 260 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; e.g. Sigma D2650) to room temperature. Add 260 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Violet laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD LSRFortessa™ or BD™ LSR II) can be used. This dye can be read out of filters commonly used for BD Horizon™ Brilliant Violet 510, BD Horizon™ V500, or AmCyan (e.g., 525\/50). Fluorescence compensation is best achieved using a sample of the cells of interest. Procedure Fixable Viability Stain 510 labeling of cells 1. Prepare cells for flow cytometry staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1x10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of the BD Horizon™ Fixable Viability Stain 510 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance, as different cell types and different applications can result in a wide degree of variability in staining. 5. Incubate the mixture for 15 minutes at room temperature protected from light. a. Optional: Incubate the cells and dye mixtures at 2-8°C for 30-60 minutes. Alternatively, incubate mixtures at 37°C for 5-7 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (e.g., FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 510 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (e.g., Cat. No. 558050, BD Phosflow™ Perm Buffer III), intracellular cytokine staining (e.g., Cat. No. 554714, BD Cytofix\/Cytoperm™ Fixation\/Permeablization Kit), or transcription factor staining (e.g., Cat. No. 562574, BD Pharmingen™ Transcription Factor Buffer Set). 5. Apoptotic cells can show variable staining. We recommend co-staining with, e.g., Annexin V APC (Cat. No. 550475) if further analysis is desired for the apoptotic cells.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400579753,"sku":"564406","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561037","title":"BD, 561037, BD Pharmingen™ APC-Cy™7 Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_396774\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, thus maximizing its fluorescence emission intensity, minimizing residual emission from APC, and minimizing required electronic compensation in multilaser-laser flow cytometry systems. Note: Although every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-Cy7 conjugate. APC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036). For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400612521,"sku":"561037","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558276","title":"BD, 558276, BD™ Cytometric Bead Array (CBA) Human IL-6 Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869132\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-6 Flex Set is a bead-based immunoassay capable of measuring human interleukin-6 (IL-6) in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IL-6 Flex Set. The biology and function of IL-6 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-6 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-6 Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. When multiplexing the BD CBA Human IL-6 Flex Set assay with the BD CBA Human LT-α Flex Set assay (Cat. No. 560083), significantly higher background will be seen in the human IL-6 assay. This increased background, while reducing the sensitivity of the human IL-6 assay, will not effect the quantitation of human IL-6 or LT-α in any other way. The BD CBA Human IL-6 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 1.6 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400645289,"sku":"558276","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558270","title":"BD, 558270, BD™ Cytometric Bead Array (CBA) Human IL-2 Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869128\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-2 Flex Set is a bead-based immunoassay capable of measuring human interleukin-2 (IL-2) in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IL-2 Flex Set. The biology and function of IL-2 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-2 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-2 Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-2 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 11.2 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. This product is manufactured and sold under license from Pestka Biomedical Laboratories, Inc. (d\/b\/a PBL InterferonSource) and may be used solely as indicated. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics is strictly prohibited. This product is covered by U.S. Patent No. 5,597,901 and Bulgarian Patent No. BG1895. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400678057,"sku":"558270","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563794","title":"BD, 563794, BD Horizon™ Brilliant Stain Buffer","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Protocols for Multicolor Immunofluorescent Staining of Cells Using BD Horizon Brilliant Stain Buffer Multicolor Staining of Human Cell Samples in Tubes or 96-Well Plates Using Individual Staining Reagents 1. Add 50 μL of BD Horizon Brilliant Stain Buffer to all tubes or desired wells for the experiment Note: The 50 μL amount of Brilliant Stain Buffer per tube or per well does not depend on the final staining volume or amount of cells used per tube or number of BD fluorescent antibodies used in staining.  Although written for use with human cells, these protocols can readily be adapted for analyzing mouse cells or cells from other species, for example, by staining mouse cells at 4°C rather than at room temperature (RT). 2. Add each fluorescent reagent at the recommended volume per test (eg, 5 μL or 20 μL) and then proceed to either Protocol 1, 2, or 3. Protocol 1 for Staining Whole Blood Samples in Tubes a. Add 100 μL of human whole blood to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 mL of BD FACS™ Lysing Solution (Cat. No. 349202; 10 min) or BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899; 15 min) per tube and incubate protected from light at RT e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer, eg, BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or BD Pharmingen™ Stain Buffer (BSA) (Cat. No. 554657) g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 2 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in Tubes a. Add 100 μL of human cells to each tube b. Vortex tube contents c. Incubate (30 min) the suspended cells protected from light at room temperature (RT) d. Add 2 ml of stain\/wash buffer per tube e. Pellet cells by centrifugation (5 min) at 1400-1500 rpm f. Aspirate supernatant; add 2-3 mL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate the supernatant and resuspend cells in 500 μL of stain\/wash buffer for flow cytometric analysis Protocol 3 for Staining Peripheral Blood Mononuclear Cells or Bulk Erythrocyte-lysed Whole Blood Samples in 96-well Plates Note:   When planning for staining in plates, the user must account for the volume of the BD Horizon Brilliant Stain Buffer used.  Although written for use with human cells, this 96-well plate-based protocol can readily be adapted for analyzing mouse cells or cells from other species. a. Add 50 μL of human cells to each well b. Incubate (30 min) protected from light at RT c. Wash by adding 100 μL of stain\/wash buffer d. Pellet cells by centrifugation (5 min) at 1400-1500 rpm e. Aspirate supernatants f. Resuspend pelleted cells by adding 250 μL of stain\/wash buffer g. Pellet cells by centrifugation (5 min) at 1400-1500 rpm h. Aspirate supernatants i. Resuspend pelleted cells thoroughly with 150 μL stain\/wash buffer by pipetting the suspended cells several times j. Transfer well contents to tubes and add additional stain\/wash buffer to the tubes as desired for flow cytometric analysis Note: Alternatively, acquire samples for flow cytometric analysis from the plate directly Multicolor Staining of Human Cell Samples in Tubes or 96-Well Plates Using Cocktailed Staining Reagents Instead of adding staining reagents individually to each tube or well of a 96-well plate, it may be desirable to add cocktailed staining reagents, ie, mixtures of two or more fluorescent staining reagents. The following protocol provides an example of how to prepare a \"per test\" 5-Color Fluorescent Antibody Cocktail that already contains BD Horizon Brilliant Stain Buffer. Human Samples:  Pre-mixed Fluorescent Reagent Cocktails For each multicolor test of cocktailed fluorescent reagents: i)  Add 50 μL of BD Horizon Brilliant Stain Buffer per test ii)  Add each fluorescent reagent at the recommended volume per test (5 μL or 20 μL) iii)  Mix reagents (especially after adding BD Horizon Brilliant reagents) iv)  Store cocktail at 4°C protected from light if it is to be used later Note: Protected from light, fluorescent reagent cocktails containing more than one Brilliant Violet and\/or Brilliant Blue reagent are best used within 24 hours after preparation when stored at 4ºC or within 4 hours when stored at room temperature. However, when more than one Brilliant Ultraviolet (BUV) reagent is in the cocktail, it is best used within 2 hours after preparation irrespective of storage temperature. Example of creating a 5-Color Fluorescent Antibody Cocktail containing 2 different Brilliant Violet™ Conjugates Final Volume per Test = 90 μL _____________________________________________________________________________________ Total Number of Tests Volume\/Test (μL) 1 3 5 10 Brilliant Stain Buffer 50 50 150 250 500 Reagent 1 (BV) 5 5 15 25 50 Reagent 2 (BV) 5 5 15 25 50 Reagent 3 5 5 15 25 50 Reagent 4 5 5 15 25 50 Reagent 5 20 20 60 100 200 Total Volume 90 90 270 450 900 _____________________________________________________________________________________ Add desired volume of Reagent Cocktail (90 μL in this 5-color example) to all tubes or wells using the protocols for staining human cells described above. Compensation and Setup BD Horizon Brilliant Stain Buffer can be used in single color compensation controls using cells. The buffer is compatible with BD™ Compbeads, however, it has not been tested with compensation beads from other vendors. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. Additionally, the most accurate compensation will be created when BD Horizon Brilliant Stain Buffer is used in all compensation controls, including Brilliant polymer and non-polymer dyes.\u003cbr\u003e\nProduct Notices: Product Notices Alexa Fluor® is a registered trademark of Life Technologies Corporation. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400710825,"sku":"563794","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558274","title":"BD, 558274, BD™ Cytometric Bead Array (CBA) Human IL-10 Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869131\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-10 Flex Set is a bead-based immunoassay capable of measuring human interleukin-10 (IL-10) in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human IL-10 Flex Set. The biology and function of IL-10 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-10 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-10 Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-10 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 0.13 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400743593,"sku":"558274","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560835","title":"BD, 560835, BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD3","description":"\u003cp\u003eAlternative Name: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3\/Leu-4 epsilon\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse BALB\/c IgG1, κ\u003cbr\u003e\nImmunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: I WT3; III 471\u003cbr\u003e\nRRID: AB_2033956\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027. This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400776361,"sku":"560835","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-563204","title":"BD, 563204, BD Horizon™ BV510 Mouse Anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nImmunogen: Human Peripheral Blood Leucocytes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: IV N816\u003cbr\u003e\nRRID: AB_2738067\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794\/566349).\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400809129,"sku":"563204","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558273","title":"BD, 558273, BD™ Cytometric Bead Array (CBA) Human TNF Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869130\u003cbr\u003e\nDescription: Description The BD™ CBA Human TNF Flex Set is a bead-based immunoassay capable of measuring human tumor necrosis factor (TNF), formerly known as tumor necrosis factor-α, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human TNF Flex Set. The biology and function of TNF has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg\/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human TNF Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human TNF Flex Set will be 2,500 pg\/ml. An example standard curve is shown in Figure 1. The BD CBA Human TNF Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human TNF Flex Set C4 560112 The BD CBA Human TNF Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/flexset. Performance Limit of Detection: The theoretical limit of detection is 0.7 pg\/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/ml, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices Source of all serum proteins is from USDA inspected abattoirs located in the United States. ProClin is a trademark of Rohm and Haas Company. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400841897,"sku":"558273","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561688","title":"BD, 561688, BD Pharmingen™ FITC Rat Anti-CD11b, 25ug","description":"\u003cp\u003eBD, 561688, BD Pharmingen™ FITC Rat Anti-CD11b, 25ug\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400874665,"sku":"561688","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561827","title":"BD, 561827, BD Pharmingen™ FITC Hamster Anti-Mouse CD3e, 25ug","description":"\u003cp\u003eBD, 561827, BD Pharmingen™ FITC Hamster Anti-Mouse CD3e, 25ug\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400907433,"sku":"561827","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558277","title":"BD, 558277, BD™ Cytometric Bead Array (CBA) Human IL-8 Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869133\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-8 Flex Set is a bead-based immunoassay capable of measuring human interleukin-8 (IL-8), also know as CXCL8, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IL-8 Flex Set. The biology and function of IL-8 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-8 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-8 Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-8 Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human OSM Flex Set D5 560084 The BD CBA Human IL-8 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 1.2 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400940201,"sku":"558277","price":0.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/files\/BD-558277.webp?v=1768804603"},{"product_id":"bd-558050","title":"BD, 558050, BD Phosflow™ Perm Buffer III","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at room temperature.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Flow cytometry: 1.   Warm BD Phosflow™ Fix Buffer I (Cat. No. 557870) to 37 °C prior to use. 2.   Chill BD Phosflow™ Perm Buffer III to -20 °C prior to use. 3.   Collect PBMCs after Ficoll-Paque separation and treat with any appropriate cell activators as applicable. 4.   At the end of the treatment, immediately mix one volume of the warmed BD Phosflow™ Fix Buffer I with one volume of the PBMC suspension. Mix well and incubate the tubes in a 37 °C water bath for 10 min.  Spin down the cells at 250 x g for 10 min in a table-top centrifuge and then aspirate the supernatant. 5.   Wash the cells once with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Alternatively, 1X Washing\/Staining Solution (1 x PBS, 1% FCS, and 0.09% sodium azide) may be used. Centrifuge to pellet the cells and remove the supernatant. 6.   Vortex to loosen the cells.  Permeabilize the cells by slowly adding cold BD Phosflow™ Perm Buffer III while vortexing.  Incubate on ice for 30 min and then spin down the cells. 7.   Wash the cells twice with 5-10 mL of BD Pharmingen™ Stain Buffer (FBS). Centrifuge at 250 x g for 10 minutes and remove the supernatant by aspiration. 8.   Resuspend cells in BD Pharmingen™ Stain Buffer (FBS) at 1x 10^7\/ml and aliquot 100 µl to each flow tube to continue with antibody staining and flow cytometric analysis. Danger: BD Phosflow™ Perm Buffer III contains 87.68% methanol (w\/w). Hazard statements Highly flammable liquid and vapor. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to the central nervous system. Route of exposure: Oral. Precautionary statements Wear protective clothing \/ eye protection. Wear protective gloves. Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. Wash thoroughly after handling. Use only outdoors or in a well-ventilated area. Keep away from heat\/sparks\/open flames\/hot surfaces. - No smoking. Ground and bond container and receiving equipment.  Use explosion-proof [electrical\/ventilating\/lighting\/] equipment. Use non-sparking tools. Take action to prevent static discharges. Do not breathe mist\/vapours\/spray. Do not eat, drink or smoke when using this product. IF ON SKIN (or hair): Remove\/Take off immediately all contaminated clothing. Rinse skin with water\/shower. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Immediately call a POISON CENTER\/doctor. Rinse mouth. If exposed: Call a poison center\/doctor. In case of fire: Use suitable extinguishing media to extinguish. Store in well-ventilated place. Keep cool. Store locked up. Keep container tightly closed.\u003cbr\u003e\nProduct Notices: Product Notices Ficoll-Paque is a trademark of Amersham Biosciences Limited. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802400972969,"sku":"558050","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564997","title":"BD, 564997, BD Horizon™ Fixable Viability Stain 700","description":"\u003cp\u003eApplication: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)\u003cbr\u003e\nRRID: AB_2869637\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Preparation Bring FVS700 dye powder and 310 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. Add 310 μl of DMSO and vortex solution well. Inspect the solution and repeat vortex until the stock dye has fully dissolved. This is the Stock Solution. Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO. Cytometry Requirements Red laser-equipped Flow Cytometers (eg, BD FACSCanto™ II, BD LSRFortessa™, or BD LSR™ II) can be used. This dye can be read out of filters commonly used for BD Horizon™ APC-R700 or Alexa Fluor® 700 (eg, 730\/45- or 712\/21-nm filter). Fluorescence compensation is best achieved using stained and unstained samples of the cells of interest. Procedure Fixable Viability Stain 700 labeling of cells 1. Prepare cells for flow cytometric staining using sodium azide-free buffers. 2. Wash cells one time in sodium azide- and protein-free Dulbecco's Phosphate Buffered Saline (1X DPBS). 3. Resuspend cells at 1-10 x 10^6 cells\/ml in sodium azide- and protein-free 1X DPBS. 4. Add 1 μl of BD Horizon™ Fixable Viability Stain 700 Stock Solution for each 1 ml of cell suspension (1:1000) and vortex immediately. a. Note: We recommend titrating the dye for optimal performance as different cell types and different applications can result in a wide degree of variability in staining. Please see Note 1 below for guidance on recommended ranges. 5. Incubate the mixture for 10-15 minutes at room temperature or 2-8°C protected from light. a. Optional: Alternatively, incubate mixtures at 37°C for 5-7 minutes. 6. Wash cells twice with 2 ml of BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or the equivalent. 7. Decant the supernatant and gently mix to disrupt the cell pellet. 8. Resuspend the cells in Stain Buffer (FBS) or equivalent. 9. Stain, fix and permeabilize cells as desired for downstream applications. Notes: 1. Each user should determine the optimal concentrations of reagents, cells, and conditions for the assay of interest. We recommend titrating the reagent in early experiments to obtain optimal results. The following are ranges that we have found to work for various cell systems: a. Erythrocyte-Lysed Whole Blood Cells: 1:1,000 from the Stock Solution. b. Primary Cells: 1:1,000 - 1:4,000 from the Stock Solution. c. Cell Lines: 1:4,000 - 1:10,000 from the Stock Solution. 2. The reactivity of the free dye is quenched by washing with buffer containing protein (eg, FBS or BSA). 3. Cells may be stained in bulk prior to freezing or staining with fluorescent antibodies. 4. BD Horizon™ Fixable Viability Stain 700 can be used in intracellular staining assays that require fixation with formaldehyde and permeabilization with methanol and detergents such as those used for BD Phosflow™ staining (eg, BD Phosflow™ Perm Buffer III, Cat. No. 558050), intracellular cytokine staining (eg, BD Cytofix\/Cytoperm™ Fixation\/Permeabilization Solution Kit, Cat. No. 554714), or transcription factor staining (eg, BD Pharmingen™ Transcription Factor Buffer Set, Cat. No. 562574\/562725). 5. Apoptotic cells can show variable staining. We recommend co-staining with other fluorescent probes such as Annexin V FITC (Cat. No. 556419) if further analysis is desired for the apoptotic cells.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401005737,"sku":"564997","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554655","title":"BD, 554655, BD Cytofix™ Fixation Buffer, 100mL","description":"\u003cp\u003eBD, 554655, BD Cytofix™ Fixation Buffer, 100mL\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401038505,"sku":"554655","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560484","title":"BD, 560484, BD™ Cytometric Bead Array (CBA) Human Th1\/Th2\/Th17 CBA Kit","description":"\u003cp\u003eReactivity: Human (QC Testing)\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869353\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store all kit components at 2 to 8°C. Do not freeze.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401104041,"sku":"560484","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554723","title":"BD, 554723, BD Perm\/Wash™ Perm\/Wash Buffer","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C. Note: BD Perm\/Wash buffer consists of 100 ml of concentrated stock solution (10X) containing both Fetal Bovine Serum (FBS) and saponin. It is not uncommon for the color of this product to vary from lot to lot. A small amount of insoluble precipitate is also common. Color variation and\/or precipitate do not affect product performance. If desired, the precipitate can be removed before use by passing the diluted 1X BD Perm\/Wash buffer through a 0.45 micron filter.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Stimulation of Cells: Various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR\/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28). Procedure for Using BD Perm\/Wash™ buffer: 1.   Fix cells using a buffer containing paraformaldehyde, or use BD Cytofix\/Cytoperm™ solution (Cat. No. 554722) 2.   Permeabilize fixed cells by washing 2 times in 1X BD Perm\/Wash buffer (Cat. No. 554723) (e.g., 1 ml\/wash for staining in tubes and 250 µl\/wash final volume for staining in microwell plates). Incubate for 15 minutes in 1X BD Perm\/Wash buffer (the 15 minute incubation can be omitted if BD Cytofix\/Cytoperm is used for fixing cells). Pellet cells. a. Dilute 10X BD Perm\/Wash buffer in distilled H2O to make a 1X solution prior to use. 3. Stain for Intracellular Cytokines a. Thoroughly resuspend fixed\/permeabilized cells in 50 µl of BD Perm\/Wash buffer containing a pre-determined optimal concentration of a fluorochrome-conjugated anti-cytokine antibody or appropriate negative control. Incubate at 4°C for 30 minutes in the dark. b. Wash cells 2 times with 1X BD Perm\/Wash buffer (1 ml\/wash for staining in tubes and 250 µl\/wash final volume for staining in microwell plates) and resuspend in staining buffer prior to flow cytometric analysis. Note: Both the BD Perm\/Wash buffer (Cat. No. 554723) and the BD Cytofix\/Cytoperm solution (Cat. No. 554722) are included in the BD Cytofix\/Cytoperm Kit (Cat. No. 554714) as well as the BD Cytofix\/Cytoperm Plus Kit with BD GolgiStop™ protein transport inhibitor (containing monensin; Cat. No. 554715) and BD Cytofix\/Cytoperm Plus™ Kit with BD GolgiPlug™ protein transport inhibitor (containing brefeldin A; Cat. No. 555028). Warning: The BD Perm\/Wash solution contains saponin and sodium azide which is known to be toxic. Avoid contact with skin, eyes and mucous membranes.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401136809,"sku":"554723","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-559763","title":"BD, 559763, BD Pharmingen™ PE Annexin V Apoptosis Detection Kit I","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869265\u003cbr\u003e\nDescription: Description Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including Phycoerythrin (PE). This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, PE Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. PE Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with PE Annexin V is typically used in conjunction with a vital dye such as 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (7-AAD negative, PE Annexin V positive). Viable cells with intact membranes exclude 7-AAD, wheras the membranes of dead and damaged cells are permeable to 7-AAD. For example, cells that are considered viable are PE Annexin V and 7-AAD negative; cells that are in early apoptosis are PE Annexin V positive and 7-AAD negative; and cells that are in late apoptosis or already dead are are both PE Annexin V and 7-AAD positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both PE Annexin V and 7-AAD. However, when apoptosis is measured over time, cells can be often tracked from PE Annexin V and 7-AAD negative (viable, or no measurable apoptosis), to PE Annexin V positive and 7-AAD negative (early apoptosis, membrane integrity is present) and finally to PE Annexin V and 7-AAD positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both PE Annexin V and 7-AAD positive, in of itself, reveals less information about the process by which the cells underwent their demise.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures PE Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. PE Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining as described in the PE Annexin V Staining Protocol. Investigators should note that PE Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types, however, have been previously reported (Casiola-Rosen et al . and van Engelend et al.). INDUCTION OF APOPTOSIS BY CAMPTOTHECIN The following protocol is provided as an illustration on how PE Annexin V may be used on a cell line (Jurkat). Materials 1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 µM) to 1 x 10^6 Jurkat cells. 2. Incubate the cells for 4-6 hr at 37°C. 3. Proceed with the PE Annexin V Staining Protocol to measure apoptosis. PE ANNEXIN V STAINING PROTOCOL PE Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment.   Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. 7-Amino-Actinomycin (7-AAD) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude 7-AAD, whereas the membranes of dead and damaged cells are permeable to 7-AAD. Cells that stain positive for PE Annexin V and negative for 7-AAD are undergoing apoptosis. Cells that stain positive for both PE Annexin V and 7-AAD are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both PE Annexin V and 7-AAD are alive and not undergoing measurable apoptosis. Reagents 1. PE Annexin V (component no. 51-65875X):  Use 5 µl per test. 2. 7-Amino-Actinomycin (7-AAD) (component no. 51-68981E) is a convenient, ready-to-use nucleic acid dye.  Use 5 µl per test. 3. 10X Annexin V Binding Buffer (component no. 51-66121E):  0.1 M Hepes\/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2.  For a 1X working solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water. Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells\/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of PE Annexin V and 5 µl 7-AAD. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. Add 400 µl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr. SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY The following controls are used to set up compensation and quadrants: 1.  Unstained cells. 2.  Cells stained with PE Annexin V (no 7-AAD). 3.  Cells stained with 7-AAD (no PE Annexin V). Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with PE Annexin V and\/or PE Annexin V and 7-AAD. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (PE Annexin V positive, 7-AAD negative or PE Annexin V positive, 7-AAD positive). The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for 7-AAD as well as for PE Annexin V. Thus the assay does not distinguish between cells that have already undergone an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with both PE Annexin V and 7-AAD.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 2.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401169577,"sku":"559763","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-552851","title":"BD, 552851, BD Pharmingen™ PerCP Mouse Anti-Human CD3, 50Tst","description":"\u003cp\u003eBD, 552851, BD Pharmingen™ PerCP Mouse Anti-Human CD3, 50Tst\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401235113,"sku":"552851","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558272","title":"BD, 558272, BD™ Cytometric Bead Array (CBA) Human IL-4 Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869129\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-4 Flex Set is a bead-based immunoassay capable of measuring human interleukin-4 (IL-4) in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IL-4 Flex Set. The biology and function of IL-4 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-4 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-4 Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-4 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 1.4 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401300649,"sku":"558272","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560112","title":"BD, 560112, BD™ Cytometric Bead Array (CBA) Human TNF Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869306\u003cbr\u003e\nDescription: Description The BD™ CBA Human TNF Flex Set is a bead-based immunoassay capable of measuring human tumor necrosis factor (TNF), formerly known as tumor necrosis factor-α, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human TNF Flex Set. The biology and function of TNF has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg\/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human TNF Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human TNF Flex Set will be 2,500 pg\/ml. An example standard curve is shown in Figure 1. The BD CBA Human TNF Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human TNF Flex Set D9 558273 BD CBA Human Angiogenin Flex Set C4 558328 The BD CBA Human TNF Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/flexset. Performance Limit of Detection: The theoretical limit of detection is 1.2 pg\/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/ml, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices Source of all serum proteins is from USDA inspected abattoirs located in the United States. ProClin is a trademark of Rohm and Haas Company. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 1.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401333417,"sku":"560112","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560274","title":"BD, 560274, BD Pharmingen™ APC-H7 Mouse anti-Human CD45","description":"\u003cp\u003eAlternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nRRID: AB_1645479\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with APC-H7 under optimum conditions, and unconjugated antibody and APC-H7 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Although BD APC-H7 is engineered to minimize spillover to the APC channel and is more stable and less affected by light, temperature, and formaldehyde-based fixatives, compared to other APC-cyanine tandem dyes, it is still good practice to minimize as much as possible, any light, temperature and fixative exposure when working with all fluorescent conjugates. BD APC-H7 is a tandem conjugate and an analog of APC-Cy7 with the same spectral properties. It has decreased intensity but it is engineered for greater stability and less spillover in the APC channel and consequently offers better performance than APC-Cy7. It has an absorption maximum of approximately 650 nm. When excited by light from a red laser, the APC fluorochrome can transfer energy to the cyanine dye, which then emits at a longer wavelength. The resulting fluorescent emission maximum is approximately 767 nm. BD recommends that a 750-nm longpass filter be used along with a red-sensitive detector such as the Hamamatsu R3896 PMT. As with APC-Cy7 special filters are required when using APC-H7 in conjunction with APC. Note: Although our APC-H7 products demonstrate higher lot-to lot consistency than other APC tandem conjugate products, and every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-H7 conjugate. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401366185,"sku":"560274","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554722","title":"BD, 554722, Fixation and Permeabilization Solution, 125mL","description":"\u003cp\u003eBD, 554722, Fixation and Permeabilization Solution, 125mL\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401398953,"sku":"554722","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561088","title":"BD, 561088, BD Pharmingen™ FITC Rat Anti-Mouse CD45","description":"\u003cp\u003eAlternative Name: Ptprc; LCA; Leukocyte common antigen; T200; Ly-5; Lyt-4\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2b, κ\u003cbr\u003e\nImmunogen: Mouse Thymus \/ Spleen\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nRRID: AB_394609\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401431721,"sku":"561088","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561109","title":"BD, 561109, BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse CD8a","description":"\u003cp\u003eAlternative Name: Cd8a; CD8 alpha chain; Ly-2; Lyt2; Lyt-2; Ly-35; Ly-B\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat LOU, also known as Louvain, LOU\/C, LOU\/M IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse Spleen Cells or Thymocyte Membranes\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_394081\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401464489,"sku":"561109","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-555214","title":"BD, 555214, BD OptEIA™ TMB Substrate Reagent Set","description":"\u003cp\u003eApplication: ELISA (Tested During Development)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869044\u003cbr\u003e\nDescription: Description The TMB substrate reagent set is designed for use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is a colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store unopened reagents at 2-8°C. Do not use after the expiration date. Discard any remaining working solutions after use. Reagents should be brought to room temperature (20-25°C) prior to use. Allow at least 30 minutes for this process. Avoid prolonged exposure to light, contact with metal, air or extreme temperatures as color may develop.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Prepare a working concentration of TMB substrate solution within 15 minutes prior to use by mixing equal volumes of Substrate Reagent A and Substrate Reagent B (e.g. for one 96-well plate, a 12 mL TMB substrate working solution can be prepared by mixing 6 mL of Substrate A with 6 mL of Substrate Reagent B).  When mixed together, TMB substrate solution should be colorless to a very faint blue. In the presence of peroxide-labeled conjugates, the solution should develop a definitive blue color. After the addition of sulfuric or phosphoric acid to stop the reaction, the color changes from blue to yellow. Danger: Substrate Reagent B (component 51-2607KC) contains 33.05% methanol (w\/w), 13.34% glycerin (w\/w), and 1.745% DMSO (w\/w). Hazard statements Flammable liquid and vapour. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to the central nervous system. Route of exposure: Oral. Causes damage to organs. Precautionary statements Keep away from heat\/sparks\/open flames\/hot surfaces. - No smoking. Keep container tightly closed. Ground and bond container and receiving equipment. Use explosion-proof [electrical\/ventilating\/lighting\/] equipment. Use non-sparking tools. Take action to prevent static discharges. Use suitable extinguishing media to extinguish. Wear protective gloves \/ eye protection. Wear protective clothing. Do not breathe mist\/vapours\/spray. Use only outdoors or in a well-ventilated area. Wash thoroughly after handling. Do not eat, drink or smoke when using this product. IF ON SKIN (or hair): Remove\/Take off immediately all contaminated clothing. Rinse skin with water\/shower.Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Call a POISON CENTER\/doctor\/ if you feel unwell. Rinse mouth. IF exposed or concerned: Call a POISON CENTER\/doctor\/ Store in a well-ventilated place. Keep cool.  Store locked up.  Keep container tightly closed. Dispose of contents\/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.\u003cbr\u003e\nProduct Notices: Product Notices Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 300.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401497257,"sku":"555214","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-554724","title":"BD, 554724, BD GolgiStop™ Protein Transport Inhibitor (Containing Monensin), 0.7mL","description":"\u003cp\u003eBD, 554724, BD GolgiStop™ Protein Transport Inhibitor (Containing Monensin), 0.7mL\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401530025,"sku":"554724","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565411","title":"BD, 565411, BD Horizon™ BV421 Rat Anti-Mouse F4\/80","description":"\u003cp\u003eAlternative Name: Gpf480; F480; Emr1; Ly71; DD7A5-7; EGF-TM7; TM7LN3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat WI, also known as Wistar (outbred) IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse F4\/80 Recombinant Protein\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested), Bioimaging (Tested During Development)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2734779\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. An isotype control should be used at the same concentration as the antibody of interest.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401562793,"sku":"565411","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565250","title":"BD, 565250, BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse CD206","description":"\u003cp\u003eAlternative Name: MMR; MR; Macrophage mannose receptor 1; Mrc1; Mannose receptor, C type 1\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat F344, also known as Fischer, CDF IgG2a\u003cbr\u003e\nImmunogen: Recombinant Mouse CD206 Carbohydrate recognition domains 4-7\/Fc Fusion Protein\u003cbr\u003e\nApplication: Intracellular staining (flow cytometry) (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2739133\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. An isotype control should be used at the same concentration as the antibody of interest.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401595561,"sku":"565250","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-552843","title":"BD, 552843, BD™ CompBeads Anti-Mouse Ig, κ\/Negative Control Compensation Particles Set","description":"\u003cp\u003eApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nEntrez Gene ID: 16071\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_10051478\u003cbr\u003e\nDescription: Description The BD™ CompBeads Set Anti-Mouse Ig, κ are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD™ CompBeads Anti-Mouse Ig, κ particles, which bind any mouse κ light chain-bearing immunoglobulin, and the BD™ CompBeads Negative Control, which has no binding capacity. When mixed together with a fluorochrome-conjugated mouse antibody, the BD™ CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Note: BD Horizon™ V500 and AmCyan conjugated reagents can show significant differences in emission spectrum on stained cells and when captured on BD™ CompBeads.  Thus, spillover values for these dyes evaluated with BD™ CompBeads may not provide correct compensation for cells.  Therefore, single stained cellular controls are recommended to set up compensation for AmCyan and BD Horizon™ V500 reagents.  BD Horizon™ V500-C has been modified to minimize these spectral differences and BD™ CompBeads may be used to determine spillover values for RUO antibodies conjugated to BD Horizon™ V500-C. Without affecting compensation function, some lots may profile as a bi-modal histogram, which may be possible due to inherent light scatter and\/or residual aggregation of the compensation particles.  Optimization of instrument voltage or gating conditions may be helpful for improving histogram visualization. This BD™ CompBeads Set has been tested with mouse Ig antibodies conjugated to various fluorochromes and analyzed using a BD FACS brand flow cytometer to ensure specificity and reactivity of the particles. See the specific instructions below on the use of the BD™ CompBeads Set: 1.    Vortex BD™ CompBeads thoroughly before use. 2.    Label a separate 12 x 75 mm sample tube for each flurochrome-conjugated mouse Ig, κ antibody to be used on a given experiment. 3.    Add 100 µl of staining buffer [e.g., BD Pharmingen Stain (FBS), Cat. No. 554656 or BD Pharmingen Stain (BSA), Cat. No. 554657] to each tube. 4.    Add 1 full drop (approximately 60 µl) of the BD™ CompBeads Negative Control and 1 drop of the BD™ CompBeads Anti-Mouse Ig, κ  beads to each tube and vortex. 5.    Add 20 µl of each prediluted antibody stock (diluted to a concentration optimal for staining 10^6 cells) to be tested on a given experiment to the appropriately-labeled tube. (Make sure the antibody is deposited to the bead mixture, then vortex.) 6.    Incubate 15 - 30 minutes at room temperature. Protect from exposure to direct light. 7.    During the incubation of beads and antibody, set the flow cytometer instrument PMT voltage settings using the target tissue for the given experiment (eg, whole blood, splenocytes, etc). If you are unsure, use the BD™ CompBeads Negative Control beads as your negative reference point and proceed. 8.    Following the incubation step (see Step 6 above), add 2 ml staining buffer to each tube and pellet by centrifugation at 200 x g for 10 minutes. 9.    Discard supernatant from each tube by careful vacuum aspiration using a fine-tip Pasteur pipette. 10.  Resuspend bead pellet in each tube by adding 0.5 ml of staining buffer to each tube. Vortex thoroughly. 11.  Run each tube separately on the flow cytometer. Gate on the singlet bead population based on FSC (forward-light scatter) and SSC (side-light scatter) characteristics. 12.  Adjust flow rate to 200 - 300 events per second if possible. 13.  Create a dot plot for the given fluorochrome-conjugated antibody as appropriate [i.e., to set compensation for a fluorescein (FITC)-conjugated antibody, use an FL1 vs. FL2 dot plot]. 14.  Place a quadrant gate such that the negative bead population is in the lower left quadrant and the positive bead population is in the upper or lower right quadrant, and adjust the compensation values until the median fluorescence intensity (MFI) of each population (as shown in the quadrant stats window) is approximately equal (i.e., for FL2 -%FL1, the FL2 MFI of both bead populations should be approximately equal when properly compensated). 15.  Repeat Steps 13 and 14 for other tubes, as necessary. 16.  Proceed to acquiring the actual staining experiment.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Cy is a trademark of Amersham Biosciences Limited. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 6.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401628329,"sku":"552843","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-562441","title":"BD, 562441, BD Horizon™ BV421 Mouse Anti-Human CD19","description":"\u003cp\u003eAlternative Name: B4; B-lymphocyte antigen CD19; Leu-12\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: V CD19.11\u003cbr\u003e\nRRID: AB_11153299\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401661097,"sku":"562441","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-550825","title":"BD, 550825, BD Pharmingen™ PI\/RNase Staining Buffer","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Do not dilute or boil.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures Flow cytometry: After fixing and permeabilizing your cell sample, use 0.5 mL \/test (1 x 10e6 cells) and incubate for 15 minutes at room temperature before analysis.  Please refer to http:\/\/static.bdbiosciences.com\/documents\/BD_FlowCytometry_DNA_Staining_Protocol.pdf for more protocol information.\u003cbr\u003e\nProduct Notices: Product Notices Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Avoid contact with skin and eyes. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401693865,"sku":"550825","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-555029","title":"BD, 555029, BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A), 1mL","description":"\u003cp\u003eBD, 555029, BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A), 1mL\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401726633,"sku":"555029","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561527","title":"BD, 561527, BD™ Accutase™ Cell Detachment Solution","description":"\u003cp\u003ePreparation And Storage: Preparation And Storage The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage. This product is shipped frozen, but may thaw during transit. On receipt, if it remains cool to the touch, it may be stored at 4 °C for up to 2 months or re-frozen and stored at -20 °C for up 2 years.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures To obtain a single-cell suspension: ( Use aseptic techniques if you plan on continuing culture of cells. ) 1. Wash the cells with PBS at room temperature. 2. Add Accutase™ cell detachment solution to adequately cover the entire area of the culture vessel. 3. Incubate at room temperature for 5 to 10 minutes, or until cells are detached. Cells can also be incubated at 37°C, however enzyme activity will decrease over time. a. Additional time and\/or Accutase™ may be needed to disassociate three-dimensional structures. 4. Triturate the cells to aid in obtaining a single-cell suspension. a. Additional cell culture medium may be added to assist in obtaining a single-cell suspension. b. The additional cell culture medium will neutralize the Accutase™ and allow for passaging of cells without additional washes. 5. Remove a small subset of the cell suspension and examine under a microscope to confirm the presence of single cells.\u003cbr\u003e\nProduct Notices: Product Notices Accutase is a registered trademark of Innovative Cell Technologies, Inc. mTESR™1 is a trademark of StemCell Technologies. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401759401,"sku":"561527","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-564713","title":"BD, 564713, BD Horizon™ BV510 Mouse Anti-Human CD3","description":"\u003cp\u003eAlternative Name: CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nVol. Per Test: 5 µl\u003cbr\u003e\nWorkshop Number: V 5T-CD03.05\u003cbr\u003e\nRRID: AB_2738909\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.\u003cbr\u003e\nProduct Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Brilliant Violet™ 510 is a trademark of Sirigen. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401792169,"sku":"564713","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-560383","title":"BD, 560383, BD™ Cytometric Bead Array (CBA) Human IL-17A Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869337\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-17A Flex Set is a bead-based immunoassay capable of measuring human Interleukin-17 (IL-17) in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human IL-17A Flex Set. The biology and function of IL-17 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-17A Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-17A Flex Set will be 2500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-17A Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Receptor Protein Flex Set reagents. For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 0.3 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : 100.0 : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401824937,"sku":"560383","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-565410","title":"BD, 565410, BD Pharmingen™ PE Rat Anti-Mouse F4\/80","description":"\u003cp\u003eAlternative Name: Gpf480; F480; Emr1; Ly71; DD7A5-7; EGF-TM7; TM7LN3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat WI, also known as Wistar (outbred) IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse F4\/80 Recombinant Protein\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_2687527\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. An isotype control should be used at the same concentration as the antibody of interest. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401857705,"sku":"565410","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-561091","title":"BD, 561091, BD Pharmingen™ APC Rat Anti-Mouse CD4","description":"\u003cp\u003eAlternative Name: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4\/Leu-3\u003cbr\u003e\nReactivity: Mouse (QC Testing)\u003cbr\u003e\nIsotype: Rat DA, also known as DA\/HA IgG2a, κ\u003cbr\u003e\nImmunogen: Mouse Thymocytes (BALB\/c)\u003cbr\u003e\nApplication: Flow cytometry (Routinely Tested)\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nRRID: AB_398528\u003cbr\u003e\nStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nPreparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.\u003cbr\u003e\nProduct Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com\/colors. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS).\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401890473,"sku":"561091","price":0.99,"currency_code":"USD","in_stock":true}]},{"product_id":"bd-558279","title":"BD, 558279, BD™ Cytometric Bead Array (CBA) Human IL-1β Flex Set","description":"\u003cp\u003eAssay Range: 10-2,500 pg\/mL\u003cbr\u003e\nReactivity: Human (QC Testing)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nRRID: AB_2869135\u003cbr\u003e\nDescription: Description The BD™ CBA Human IL-1β Flex Set is a bead-based immunoassay capable of measuring human interleukin-1β (IL-1β) in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human IL-1β Flex Set. The biology and function of IL-1β has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).\u003cbr\u003e\nPreparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg\/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.\u003cbr\u003e\nRecommended Assay Procedures: Recommended Assay Procedures The BD CBA Human IL-1β Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions on the use of this product can  be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-1β Flex Set will be 2,500 pg\/mL. An example standard curve is shown in Figure 1. The BD CBA Human IL-1β Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http:\/\/www.bdbiosciences.com\/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 2.3 pg\/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg\/mL, n =30) + 2 standard deviations.\u003cbr\u003e\nProduct Notices: Product Notices ProClin is a trademark of Rohm and Haas Company. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w\/w) of a CMIT\/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves\/eye\/face protection. Wear protective clothing. Avoid breathing mist\/vapours\/spray. If skin irritation or rash occurs: Get medical advice\/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents\/container in accordance with local\/regional\/national\/international regulations. Please refer to http:\/\/regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com\/us\/s\/resources for technical protocols.\u003cbr\u003e\nDescription : Quantity\/Size : Part Number : EntrezGene ID\u003cbr\u003e\nN\/A : null : N\/A : N\/A\u003c\/p\u003e","brand":"BD","offers":[{"title":"Default Title","offer_id":45802401923241,"sku":"558279","price":0.99,"currency_code":"USD","in_stock":true}]}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0653\/8847\/8633\/collections\/bd-bioscience-image.jpg?v=1762525951","url":"https:\/\/iright.com\/collections\/bd-reagents-collection.oembed?page=59","provider":"Iright","version":"1.0","type":"link"}