Cytiva, SH31120.07, HyClone™ Cell Boost™ 7b Supplement

HyClone Cell Boost 7b Liquid, 20 L bag Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications. • Designed to support modern manufacturing processes, Cell Boost™ 7b supplement is a concentrated solution of amino acids with an alkaline pH. Supplement is chemically defined, animal-derived component-free (ADCF), do not contain any growth factors (such as insulin), peptides, hydrolysates, phenol red, or 2-mercaptoethanol. • Use Cell Boost™ 7a and 7b together to achieve high yields of mAbs and other proteins in CHO fed-batch processes. • For convenience, this cell culture supplement is available as a ready-to-use liquid feed solution in addition to a dry powder format. Improve productivity and cell yield in fed-batch cultures by supplementing the cell culture medium to replace nutrients depleted during cell culture. HyClone™ Cell Boost™ 7a and Cell Boost™ 7b supplements are intended for use in combination with HyClone™ ActiPro and ActiSM media as part of the ActiPro cell culture system formulated to provide high yields of recombinant proteins in processes when using Chinese hamster ovary (CHO) cell lines. Cell Boost™ 7b is available as a convenient ready-to-use liquid feed solution or as a dry powder feed to be hydrated before use. Fed-batch applications This HyClone™ cell culture supplement has been developed for high-yield recombinant protein production when using various Chinese hamster ovary (CHO) cell lines. Composition of HyClone™ Cell Boost™ 7b cell culture media supplement Cell Boost™ 7b CHO supplement has an alkaline pH and is a concentrated solution of amino acids. Like Cell Boost™ 7a, this media supplement does not contain any growth factors (such as insulin), peptides, hydrolysates, phenol red, or 2-mercaptoethanol ensuring batch-to-batch consistency and increased cell culture process efficiency. Applications data for CHO bioreactor cultures using HyClone™ Cell Boost™ supplements A recent study compared cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone™ ActiPro basal medium and Cell Boost™ 7a and 7b media supplements. Results from shake flask cultures showed high viable cell densities and protein production. In addition, scalability of the culture process was demonstrated in 2 and 50 L bioreactor cultures . Cell density and protein production were shown to be comparable between scales. Protein yields reached as high as 5 g/L in shake flasks, and as high as 5.5 g/L in bioreactor cultures. Dry powdered medium milling equivalency study This study compares pin milling of powdered HyClone™ cell culture media and supplements at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.