{"product_id":"abcam-ab100533","title":"Abcam, ab100533, Human hCG ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman hCG ELISA Kit is a sandwich ELISA designed to quantify Human hCG with a sensitivity of 50 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 54.87 - 40000 pg\/mL - Cited in over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 50 pg\/mL,\u003cbr\u003e\nRange:54.87 - 40000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman hCG ELISA Kit ab100533 is a sandwich ELISA to measure Human hCG in serum, plasma, cell culture supernatant with a sensitivity of 50 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman hCG ELISA Kit ab100533 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature\u003cbr\u003e\n2. Add prepared biotin antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Add prepared Streptavidin solution. Incubate at room temperature\u003cbr\u003e\n4. Add TMB One-Step Development Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450nm immediately.\u003cbr\u003e\nOptimization may be required with urine samples\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHuman chorionic gonadotropin (hCG) is a glycoprotein hormone composed of two subunits alpha and beta. The beta subunit confers its specific biological activity. Its molecular weight ranges around 36.7 kDa. hCG is produced in the placenta during pregnancy and is also expressed in pituitary cells. Known alternatively as the pregnancy hormone hCG plays a critical role in maintaining the luteal function during the early stages of pregnancy.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHCG regulates several functions related to reproduction. It supports the corpus luteum in ovaries to secrete progesterone during early pregnancy contributing to the uterine lining's maintenance. Moreover hCG can interact with the luteinizing hormone\/chorionic gonadotropin receptor (LHCGR) indicating its involvement in broader reproductive functions. Not as well known hCG may exist in various forms such as hCG intact influencing certain biological pathways differently.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists study hCG's role in reproductive signaling and gonadotropin pathways. It interacts closely with luteinizing hormone (LH) with substantial overlap in their pathway functions impacting reproductive health and fertility cycles. hCG's activation of specific receptors like LHCGR plays a role in these pathways connecting it to various fertility-related responses in the human body.\u003cbr\u003e\nHCG levels can be an indicative marker for certain conditions. Elevated hCG levels may signal trophoblastic diseases such as molar pregnancy or gestational trophoblastic neoplasia. Its abnormal expression might also indicate testicular cancer where it acts as a tumor marker. Both conditions relate to hCG through its shared glycoprotein structure with other hormones like thyroid-stimulating hormone (TSH) which links the hormone to broader endocrine disorder research.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843616952489,"sku":"ab100533","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab100533","provider":"Iright","version":"1.0","type":"link"}