{"product_id":"abcam-ab100617","title":"Abcam, ab100617, Human Osteoprotegerin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Osteoprotegerin ELISA Kit is a sandwich ELISA designed to quantify Human Osteoprotegerin with a sensitivity of 1 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 1.23 - 900 pg\/mL - Cited in over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum, Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 1 pg\/mL,\u003cbr\u003e\nRange:1.23 - 900 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Osteoprotegerin ELISA Kit ab100617 is a sandwich ELISA to measure Human Osteoprotegerin in serum, plasma, cell culture supernatant, cell lysate with a sensitivity of 1 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nProduced using a non-baculovirus expression system.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman Osteoprotegerin ELISA Kit ab100617 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared streptavidin solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Development Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450nm immediately.\u003cbr\u003e\nGet results in 90 minutes with our SimpleStep ELISA\u003cbr\u003e\nHuman Osteoprotegerin ELISA Kit (\u003cbr\u003e\nab189580\u003cbr\u003e\nOptimization may be required with urine samples.\u003cbr\u003e\nWe recommend preparing serum-free or low-serum medium samples, as serum tends to contain cytokines which may produce significant background signals. If it is necessary to test serum-containing medium, we recommend also running an uncultured media blank to assess baseline signals. This baseline can then be subtracted from the cultured media sample data.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOsteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nOPG operates within the RANK\/RANKL\/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL's involvement hints towards a broader immune regulation role for OPG.\u003cbr\u003e\nOPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL\/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843619246249,"sku":"ab100617","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab100617","provider":"Iright","version":"1.0","type":"link"}