{"product_id":"abcam-ab100687","title":"Abcam, ab100687, Mouse HGF ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse HGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse HGF in Mouse in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 100 pg\/mL,\u003cbr\u003e\nRange:0.25 - 60 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of mouse HGF in cell lysates and tissue lysates.\u003cbr\u003e\nThis assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.\u003cbr\u003e\nPathways\u003cbr\u003e\nHGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.\u003cbr\u003e\nHGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843637563561,"sku":"ab100687","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab100687","provider":"Iright","version":"1.0","type":"link"}