{"product_id":"abcam-ab102500","title":"Abcam, ab102500, Hydrogen Peroxide Assay Kit","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nHydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples. - Cited in \u0026gt; 95 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.04 µM,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.\u003cbr\u003e\nThe detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 with the more sensitive fluorometric readout.\u003cbr\u003e\nThe kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.\u003cbr\u003e\nHydrogen peroxide assay principle\u003cbr\u003e\nIn the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex\/Em=535\/587 nm).\u003cbr\u003e\nHydrogen peroxide assay protocol summary:\u003cbr\u003e\n- Add standards and samples to wells\u003cbr\u003e\n- Add reaction mix and incubate for 10 min\u003cbr\u003e\n- Analyze with microplate reader\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nHydrogen Peroxide Assay Kit has been used in a variety of sample type including:\u003cbr\u003e\n- Mouse breast tumor cells\u003cbr\u003e\n- Mouse embryonic fibroblasts\u003cbr\u003e\n- Vaginal strains\u003cbr\u003e\nReferences:\u003cbr\u003e\n1- Zhou N et al. 2023; 2- Venit T et al. 2023\u003cbr\u003e\n3- Takano T et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee other alternative kits to quantify Hydrogen Peroxide:\u003cbr\u003e\n- Hydrogen Peroxide Assay Kit (Cell-based)\u003cbr\u003e\nab138874\u003cbr\u003e\n- Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared)\u003cbr\u003e\nab138886\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K265 Biovision Hydrogen Peroxide Colorimetric\/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nHydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843597357225,"sku":"ab102500","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab102500","provider":"Iright","version":"1.0","type":"link"}