{"product_id":"abcam-ab102509","title":"Abcam, ab102509, Ammonia Assay Kit - Modified Berthelot - (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nAmmonia Assay Kit (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay using a modified Berthelot reagent to measure ammonia. - Ready-to-use solutions - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Urine, Plasma, Cell culture media, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026lt; 10 µM,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAmmonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis ammonia assay using a modified Berthelot reagent is based on a reaction between ammonia and an alkaline reagent that produces a blue-green colored product. The intensity of the color is proportional to the amount of ammonia in the sample. The colored product is indophenol, which is easily quantifiable by colorimetry (λ=670nm) using a plate reader.\u003cbr\u003e\nBerthelot ammonia assay protocol summary\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reagent 1 and reagent 2 and incubate for 30 min at at 37°C\u003cbr\u003e\n- Analyze with a microplate reader\u003cbr\u003e\nHow other researchers are using ab102509\u003cbr\u003e\nAmmonia Assay Kit - Modified Berthelot (Colorimetric) has been used with a variety of sample types including:\u003cbr\u003e\nEscherichia coli BL21(DE3) cells\u003cbr\u003e\nUreaplasma cells\u003cbr\u003e\nHuman iPS(Foreskin)\u003cbr\u003e\nReferences: 1-Srinivasachari S et al. 2023, 2-Fleming D et al. 2022, 3-Pettinato G et al. 2021\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nAmmonia assay kit\u003cbr\u003e\nab83360\u003cbr\u003e\nis an alternative to this product, and is the most popular ammonia assay kit.\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K470 Biovision Ammonia Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAmmonia a small molecule with a molecular mass of about 17 daltons is an important player in nitrogen metabolism. It exists primarily as ammonium (NH4+) in physiological pH environments. Ammonia is found predominantly in liver cells where it is produced through amino acid deamination and is further involved in urea production. Ammonia detection can be performed using various tools such as the ammonia detection kit ammonia test kit and the ammonia measurement reader which are essential for researchers to analyze and quantify its presence.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAmmonia participates in the nitrogen cycle being a substrate for urea synthesis in the liver a vital part of nitrogen waste removal. It combines with carbon dioxide to form carbamoyl phosphate which enters the urea cycle a complex enzymatic system within mitochondria and cytosol. Ammonia's role is not isolated; it interacts with enzymes such as glutamine synthetase. The ammonium assay and ammonia assay provide insights into ammonia's role and concentration helping elucidate its biological functions and impact.\u003cbr\u003e\nPathways\u003cbr\u003e\nAmmonia is important in the urea cycle and gluconeogenesis. In the urea cycle it is converted to urea preventing toxic accumulation. Gluconeogenesis involves ammonia as it aids in converting pyruvate to glucose a fundamental process for energy maintenance. Related proteins in these pathways include carbamoyl phosphate synthetase in the urea cycle and pyruvate carboxylase in gluconeogenesis. A colorimetric assay might be used to evaluate ammonia's integration in these pathways.\u003cbr\u003e\nAmmonia plays a significant role in hepatic encephalopathy and hyperammonemia both linked with improper ammonia metabolism. Excess ammonia in the blood due to liver dysfunction can lead to hepatic encephalopathy affecting brain function. Glutamine synthetase activity correlates to ammonia levels impacting the severity of these conditions. Ammonia assay kits are fundamental tools in assessing the association of ammonia with these disorders facilitating better understanding and management of the conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843626881193,"sku":"ab102509","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab102509","provider":"Iright","version":"1.0","type":"link"}