{"product_id":"abcam-ab102517","title":"Abcam, ab102517, Glucose Assay Kit - reducing agent compatible","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlucose Assay Kit ab102517 provides a method for measurement of glucose in biological samples; this method is recommended for serum and urine samples as it is not affected by reducing substances.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.02 mM,\u003cbr\u003e\nRange:0.02 - 10 mM,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlucose Assay Kit ab102517 provides direct measurement of glucose in biological samples. It is particularly suitable for serum and urine samples since it is not affected by reducing substances, which can interfere with detection in oxidase-based kits.\u003cbr\u003e\nGlucose assay principle\u003cbr\u003e\nIn the glucose assay protocol, glucose is acted on by glucose dehydrogenase. The reaction is coupled by NADH to the generation of a colored dye (450 nm) whose intensity is proportional to glucose concentration.\u003cbr\u003e\nThe method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes.\u003cbr\u003e\nThe kit can detect glucose concentrations in the range of 20uM-10mM.\u003cbr\u003e\nGlucose assay protocol summary:\u003cbr\u003e\n- add reaction mix to sample and standard wells\u003cbr\u003e\n- incubate for 30 min\u003cbr\u003e\n- analyze with a microplate reader\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K686 Biovision Glucose Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlucose often referred to as blood sugar is a simple sugar and an essential carbohydrate in biology. It has a molecular mass of 180.16 g\/mol and is highly soluble in water. Glucose circulates in the bloodstream and is absorbed by tissues mainly liver muscle and adipose tissue. It serves as a critical energy source and cells use glucose uptake processes to transport glucose across their membranes. Various diagnostic tools and kits such as glucose assay kits and glucose test kits help measure glucose levels in biological samples.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlucose serves as the primary energy substrate for cells providing energy through glycolysis and oxidative phosphorylation. It is not part of any protein complexes but it interacts with numerous enzymes and proteins to regulate metabolic processes. Glucose operates in maintaining homeostasis and the brain relies on it almost exclusively for energy. Glucose assay reagents and glucose detection kits are utilized to quantify glucose concentrations in research studies examining these functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlucose is a central component in glycolysis and the tricarboxylic acid (TCA) cycle. In glycolysis glucose is broken down into pyruvate generating ATP and NADH in the process. This pathway involves key regulatory proteins such as hexokinase and phosphofructokinase. In the TCA cycle glucose metabolites further produce ATP and CO2 involving enzymes like citrate synthase. Glucose uptake assays provide insights into how these pathways operate under various physiological conditions.\u003cbr\u003e\nGlucose regulation and metabolism are tightly linked to diabetes mellitus and metabolic syndrome. Diabetes mellitus is characterized by impaired glucose uptake and insulin regulation often involving insulin receptor pathways. Persistent high glucose levels lead to complications such as neuropathy and retinopathy. AMP-activated protein kinase (AMPK) plays an important role in metabolic syndrome by affecting glucose uptake and energy balance. Understanding glucose's role in these diseases is central to devising therapeutic strategies and interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843615150249,"sku":"ab102517","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab102517","provider":"Iright","version":"1.0","type":"link"}