{"product_id":"abcam-ab104129","title":"Abcam, ab104129, Mounting Medium With PI - Aqueous, Fluoroshield","description":"\u003cp\u003eSize: 20mL \/ 100mL\u003cbr\u003e\nMounting Medium with PI (Propidium iodide) (ab104129) is a ready-to-use aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. Suitable to prevent rapid photobleaching for IHC, ISH and ICC\/IF applications. - Compatible with our range of fluorescent labeled secondary antibodies - Available in different sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.09% Sodium azideConstituents: 84% Water, 0.0002% Propidium Iodide\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nFluoroshield mounting medium with PI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with PI which is a counter-stain for DNA. This product is to be used in\u003cbr\u003e\nin situ\u003cbr\u003e\nhybridization techniques or other methods where fluorescence of DNA staining is required. PI excites at 535nmn and emits at 615nm, producing a red fluorescence. RNA is also stained with PI.\u003cbr\u003e\nProcedure\u003cbr\u003e\nBring the vial to room temperature.\u003cbr\u003e\nRinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.\u003cbr\u003e\nTurn the vial upside down and remove any air bubbles.\u003cbr\u003e\nApply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel.\u003cbr\u003e\nLet stand at room temperature for about 5 minutes.\u003cbr\u003e\nApply cover slip, carefully avoiding air bubbles.\u003cbr\u003e\nThe specimen is ready for visualization under microscope.\u003cbr\u003e\nOne can seal the edges of cover slip with nail polish or any organic medium or Organo mounting medium (\u003cbr\u003e\nab104141\u003cbr\u003e\n). If coverslip is not sealed air bubbles will appear in few days.\u003cbr\u003e\nFor long term storage it is recommended that the slide be stored in the dark at 2-8°C.\u003cbr\u003e\nRemoval of Coverslip\u003cbr\u003e\nCoverslip can be removed before sealing the edges. Soak slide in warm (37°C) water for a few minutes. Carefully and slowly move the coverslip. Soak in water an additional few minutes and remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.\u003cbr\u003e\nMounting Medium with PI (Propidium iodide) ab104129 (previously called Fluoroshield Mounting Medium with Propidium Iodide) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with propidium iodide as a counter-stain for DNA.\u003cbr\u003e\nThe formulation prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, R-Phycoerythrin (R-PE), Phyocyanin (PC), and Allophycocyanin (APC).\u003cbr\u003e\nFluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.\u003cbr\u003e\nMounting media products\u003cbr\u003e\nFor fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium\u003cbr\u003e\nab104135\u003cbr\u003e\n, Mounting Medium with DAPI\u003cbr\u003e\nab104139\u003cbr\u003e\n, and this product, Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI\u003cbr\u003e\nab188804\u003cbr\u003e\nFor chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium\u003cbr\u003e\nab64230\u003cbr\u003e\n, or organic Limonene Mounting Medium\u003cbr\u003e\nab104141\u003cbr\u003e\nProcedure\u003cbr\u003e\nBring the vial to room temperature.\u003cbr\u003e\nRinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.\u003cbr\u003e\nTurn the vial upside down and remove any air bubbles.\u003cbr\u003e\nApply 3-4 drops directly of mounting medium on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip, making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide on paper towel.\u003cbr\u003e\nLet stand at room temperature for about 5 minutes.\u003cbr\u003e\nApply cover slip, carefully avoiding air bubbles.\u003cbr\u003e\nThe specimen is ready for visualization under microscope.\u003cbr\u003e\nOne can seal the edges of cover slip with nail polish or any organic medium or Organo mounting medium (\u003cbr\u003e\nab104141\u003cbr\u003e\n). If coverslip is not sealed air bubbles will appear in few days.\u003cbr\u003e\nFor long term storage it is recommended that the slide be stored in the dark at 2-8°C.\u003cbr\u003e\nRemoval of Coverslip\u003cbr\u003e\nCoverslip can be removed before sealing the edges. Soak slide in warm (37°C) water for a few minutes. Carefully and slowly move the coverslip. Soak in water an additional few minutes and remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze, Store in the dark\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571951785,"sku":"ab104129","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab104129","provider":"Iright","version":"1.0","type":"link"}