{"product_id":"abcam-ab10530","title":"Abcam, ab10530, Anti-Nucleophosmin antibody [FC82291]","description":"\u003cp\u003eSize: 50µL\u003cbr\u003e\nAnti-Nucleophosmin antibody [FC82291] (ab10530) is a mouse monoclonal antibody detecting Nucleophosmin in  Western Blot, ICC\/IF . Suitable for  African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat . - Over 110 publications - Trusted since 2004\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:FC82291,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Native Full Length Protein corresponding to Rat Npm1.P13084,\u003cbr\u003e\nEpitope:The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Nucleophosmin antibody [FC82291] (ab10530) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry\/immunofluorescence (ICC\/IF) in African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Nucleophosmin?\u003cbr\u003e\nAnti-Nucleophosmin [FC82291] (ab10530) specifically detects a band for Nucleophosmin (UniProt: P06748) at a molecular weight of 33kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Nucleophosmin [FC82291] (ab10530) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-Nucleophosmin [FC82291] (ab10530) has over 25 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNucleophosmin also known as NPM1 or B23 is a multifunctional nucleolar phosphoprotein with a molecular weight of approximately 37 kDa. It is highly expressed in the nucleolus of many human cells and is involved in various cellular processes. NPM1 aids ribosome biogenesis intracellular transport of ribosomal components and regulation of the centrosome. Its nucleocytoplasmic shuttling ability is due to its N-terminal domain which plays an important role in binding to nucleic acids and other proteins. Apart from these functions NPM1 acts as a histone chaperone and is important for maintaining genomic integrity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOne of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF\/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.\u003cbr\u003e\nNPM1 mutations are commonly observed in acute myeloid leukemia (AML) where they affect the normal function of the protein leading to leukemogenesis. NPM1 is also linked to tumorigenesis through its impact on the p53 tumor suppressor pathway. Specifically alterations in NPM1 can disrupt the balance of other related proteins like ALK and p53 influencing the onset and progression of leukemia. Understanding the roles of NPM1 in these contexts is essential for developing targeted therapies to treat AML and other related disorders effectively.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844225519785,"sku":"ab10530","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab10530","provider":"Iright","version":"1.0","type":"link"}