{"product_id":"abcam-ab108849","title":"Abcam, ab108849, Mouse Fibronectin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Fibronectin ELISA Kit is a sandwich ELISA designed to quantify Mouse Fibronectin with a sensitivity of 0.91 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 6.25 - 100 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.91 ng\/mL,\u003cbr\u003e\nRange:6.25 - 100 ng\/mL,\u003cbr\u003e\nAssay time:3h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Fibronectin ELISA Kit ab108849 is a sandwich ELISA to measure Mouse Fibronectin in serum, plasma, urine with a sensitivity of 0.91 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse Fibronectin ELISA Kit ab108849 protocol summary\u003cbr\u003e\n1. Add standard or sample to appropriate wells. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature\u003cbr\u003e\n4. Add Chromogen Substrate to each well. Incubate at room temperature\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450 nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843625373865,"sku":"ab108849","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab108849","provider":"Iright","version":"1.0","type":"link"}