{"product_id":"abcam-ab109876","title":"Abcam, ab109876, Complex IV Human Enzyme Activity Dipstick Assay Kit","description":"\u003cp\u003eSize: 30Test\u003cbr\u003e\nComplex IV Human Enzyme Activity Dipstick Assay Kit immunoassay kit for the detection of Complex IV Human Enzyme Activity Dipstick Assay Kit in Cow, Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Cell culture extracts, Tissue,\u003cbr\u003e\nReacts with:Cow, Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nContains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.\u003cbr\u003e\nThe isolation of mitochondria is not necessary for the performance of this assay. In this kit the specificity of anti-COX monoclonal antibodies (mAbs) is combined with traditional methods for determining COX enzyme activity by histochemical methods and in-gel activity assays. First, the COX enzyme complex is immnocaptured (i.e immunoprecipated in active form) on the dipstick. Second, the dipstick is immersed in COX activity buffer containing reduced cytochrome c and di-amino benzidinetetrachloride (DAB), which serves as the reporter of COX activity. Immunocaptured COX oxidizes cytochrome c, which then oxidizes DAB to form a red-colored precipitate at the COX antibody line on the dipstick. In addition to being quick, the reaction is cyanide-sensitive. The signal intensity of this precipitate corresponds to the level of COX activity in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.\u003cbr\u003e\nStore dipsticks at room temperature in their provided container and out of direct sunlight. High humidity conditions should be avoided.\u003cbr\u003e\nStore Buffer A, B, and C at 4°C or at -20°C for long term storage.\u003cbr\u003e\nStore Tubes 1 and 2 at -80°C; they can also be aliquoted upon receipt to prevent freeze\/thaw cycles.\u003cbr\u003e\nTube 3 can be stored at room temperature.\u003cbr\u003e\nRange of complex IV \/ cytochrome c oxidase assay kits\u003cbr\u003e\nBiochemical assay -\u003cbr\u003e\nab239711\u003cbr\u003e\nImmunocapture with biochemical assay (plate-based) -\u003cbr\u003e\nab109911\u003cbr\u003e\n(rodent) and\u003cbr\u003e\nab109909\u003cbr\u003e\n(human)\u003cbr\u003e\nImmunocapture with biochemical assay (dipstick) -\u003cbr\u003e\nab109878\u003cbr\u003e\n(rodent) and ab109876 (human) (this kit)\u003cbr\u003e\nImmunocapture with biochemical assay and ELISA -\u003cbr\u003e\nab109910\u003cbr\u003e\n(human)\u003cbr\u003e\nELISA -\u003cbr\u003e\nab179880\u003cbr\u003e\n(human)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.\u003cbr\u003e\nPathways\u003cbr\u003e\nComplex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.\u003cbr\u003e\nComplex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer’s disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843620196521,"sku":"ab109876","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab109876","provider":"Iright","version":"1.0","type":"link"}