{"product_id":"abcam-ab111622","title":"Abcam, ab111622, Janus Green cell normalization stain","description":"\u003cp\u003eSize: 55mL\u003cbr\u003e\nReady-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:In-Cell ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: Water, 0.81% Sodium chloride, 0.3% Janus Green B, 0.216% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nReady-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).\u003cbr\u003e\nCells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847577194665,"sku":"ab111622","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab111622","provider":"Iright","version":"1.0","type":"link"}