{"product_id":"abcam-ab112129","title":"Abcam, ab112129, Calcium Flux Assay Kit (Fluo-8, No Wash)","description":"\u003cp\u003eSize: 96Test \/ 10Kit\u003cbr\u003e\nCalcium Flux Assay Kit (Fluo-8) ab112129 is a no-wash, fluorescence-based assay for detecting intracellular calcium mobilization in cell cultures. - Cited in \u0026gt;50 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nFluo-8 Calcium Flux Assay Kit ab112129 is a no-wash, fluorescence-based assay for detecting intracellular calcium mobilization in cells.\u003cbr\u003e\nThis product is optimized to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.\u003cbr\u003e\nReaders with an in built pipetter are essential for measuring calcium response accurately due to transient property of the calcium signal. Depending on dose of the agonist, the peak response is few seconds to 20 seconds, so one will need to add the compound by instrument and read the fluorescent signal simultaneously every second for ~1-2 min.\u003cbr\u003e\nCalcium flux assay principle\u003cbr\u003e\nIn the Fluo-8 Calcium Flux Assay Kit protocol, cells are pre-loaded with Fluo-8 which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-8 are cleaved by an esterase, resulting in a negatively charged fluorescent dye that stays inside the cell. Its fluorescence is greatly enhanced upon binding to calcium.If intracellular calcium levels increase, the fluorescence of Fluo-8 is significantly increased.\u003cbr\u003e\nThe characteristics of its long wavelength, high sensitivity, and \u0026gt;100 times fluorescence enhancement make Fluo-8 the brightest green calcium indicator available, and it is an ideal tool for the measurement of cellular calcium through HTS screening.\u003cbr\u003e\nFluo-8 Calcium Flux assay protocol summary\u003cbr\u003e\n- Prepare cells in growth medium with 1-5% FBS\u003cbr\u003e\n- Add Fluo-8 NW dye-loading solution\u003cbr\u003e\n- Incubate at room temperature for 1 hour\u003cbr\u003e\n- Monitor fluorescence intensity at Ex\/Em = 490\/525 nm\u003cbr\u003e\nRelated Fluo-8 assay kits\u003cbr\u003e\nFluo-8 Calcium Flux Assay Kits are available in No Wash and Medium Removal variants:\u003cbr\u003e\n- No wash kit Fluo-8 Calcium Flux Assay Kit ab112129 is generally preferred as the workflow is simpler. It is also recommended for cell lines that are only weakly adherent and risk losing adherence when washing.\u003cbr\u003e\n- Medium removal kit Fluo-8 Calcium Flux Assay Kit\u003cbr\u003e\nab112128\u003cbr\u003e\nis recommended for the testing of compounds, receptors and calcium channels that are sensitive to the presence of cell culture media or serum. Alternatively, for cells that can tolerate it, consider using a low serum % in culture media and use No Wash Fluo-8 kit ab112129.\u003cbr\u003e\nFluo-8 is also available as a standalone biochemical\u003cbr\u003e\nab142773\u003cbr\u003e\nIf you would like to quantify calcium concentration in vitro using cell extracts, we recommend using Calcium Detection Kit (Colorimetric) (\u003cbr\u003e\nab102505\u003cbr\u003e\n) or Calcium Quantification Assay Kit (\u003cbr\u003e\nab112115\u003cbr\u003e\nHow other researchers are using ab112129\u003cbr\u003e\nab112129 has been used in a variety of sample types including:\u003cbr\u003e\n- Human OA chondrocytes and C28I2 cells 1\u003cbr\u003e\n-Neuronal cells 2\u003cbr\u003e\n- Human U2OS, HeLa, BJ, BJ-5ta (BJ-T), T98G, U87 MG and MRC5 cell lines and mouse embryonic fibroblasts (MEF) 3\u003cbr\u003e\nReferences: 1- Fu W et al. 2024; 2- Lui L et al. 2023; 3- Munk S et al. 2023\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843609088169,"sku":"ab112129","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab112129","provider":"Iright","version":"1.0","type":"link"}