{"product_id":"abcam-ab112139","title":"Abcam, ab112139, Lysyl Oxidase Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 500Test\u003cbr\u003e\nLysyl Oxidase Activity Assay Kit \/ LOX Activity Assay Kit (Fluorometric) (ab112139) provides a simple method to measure lysyl oxidase (LOX) activity in cell and tissue extracts from mammals and other species, as well as physiological solutions.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts, Cell culture supernatant,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nSensitivity:= 40 ng\/well,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nLysyl Oxidase Activity Assay Kit \/ LOX Activity Assay Kit (Fluorometric) (ab112139) provides a simple method to measure lysyl oxidase (LOX) activity in cell and tissue extracts from mammals and other species, as well as physiological solutions.\u003cbr\u003e\nThe LOX activity assay protocol uses a proprietary LOX substrate that releases hydrogen peroxide upon transformation by the LOX present in the sample. Hydrogen peroxide is in turn detected using a red fluorescence substrate for HRP-coupled reactions. This leads to increase in fluorescence that can be easily detected at Ex\/Em 540\/590 nm in a fluorescence microplate reader.\u003cbr\u003e\nThis assay is semi-quantitative as it does not contain a LOX standard for calibration. When a known concentration of LOX is used, the assay can detect activity from as low as 40 ng of lysyl oxidase in solution.\u003cbr\u003e\nThe assay is highly sensitive and its unique detection method eliminates the interference that occurs in certain biological samples.\u003cbr\u003e\nLysyl oxidase (protein-lysine-6-oxidase, LOX, EC 1.4.3.13) is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors.\u003cbr\u003e\nThe activity of Lysyl oxidase in biological samples is traditionally assessed by tritium release end-point assays using radio isotope labeled collagen or elastin substrates.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843610562729,"sku":"ab112139","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab112139","provider":"Iright","version":"1.0","type":"link"}