{"product_id":"abcam-ab112996","title":"Abcam, ab112996, Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMouse Monoclonal L-lactate dehydrogenase B antibody. Suitable for IP, Flow Cyt, ICC\/IF and reacts with Human samples. Cited in 3 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:10E6AA9,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt, IP, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSpecificity:Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Proprietary technique, Purification notes-ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (\u0026gt;95%)., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: 99.98% HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLactate dehydrogenase (LDH) consists largely of two subunits LDHA and LDHB also known as LDH-A and LDH-B. These subunits form tetramers in various combinations with the common forms being LDH-5 (A4) and LDH-1 (B4). LDHA consists of 332 amino acids and has a molecular mass of about 36 kiloDaltons while LDHB is of similar size. LDHA and LDHB are widely expressed in tissues with LDHA prevalent in muscle tissues and LDHB in heart tissues. Their primary function is to catalyze the interconversion of pyruvate to L-lactate and vice-versa critical in anaerobic glycolysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese LDH subunits play an important role in energy production under low oxygen conditions by allowing glycolysis to continue. The LDHA and LDHB subunits associate to form the active enzyme complex which can exist in different isoforms based on tissue-specific expression. This enzyme enables cells in oxygen-deprived conditions such as in muscles during extensive exercise or in tumors to regenerate NAD+ from NADH supporting anaerobic metabolic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nLDHA and LDHB are key players in the glycolytic pathway and gluconeogenesis. They regulate the conversion between pyruvate and lactate impacting the Cori cycle where lactate is transported to the liver for glucose production. This step links them to other metabolic pathways and proteins like the pyruvate kinase enzyme and the tricarboxylic acid cycle intermediates facilitating energy production.\u003cbr\u003e\nAltered LDHA and LDHB expression links to conditions such as cancer and ischemic tissue damage. Many tumors exhibit increased LDHA expression supporting rapid energy production via anaerobic glycolysis. In cardiac disorders high LDHB levels can indicate heart damage such as a myocardial infarction. Both LDHA and LDHB are therefore important markers and potential therapeutic targets in these disease contexts interacting closely with proteins like hexokinase and glucose transporters.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854507430057,"sku":"ab112996","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab112996","provider":"Iright","version":"1.0","type":"link"}