{"product_id":"abcam-ab117131","title":"Abcam, ab117131, Hydroxymethylated DNA Quantification Kit (Fluorometric)","description":"\u003cp\u003eSize: 48Test \/ 96Test\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt;= 10 pg\/well,\u003cbr\u003e\nAssay time:3h 20m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in\u003cbr\u003e\n5-methylcytosine (5-mC)\u003cbr\u003e\n. In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide,\u003cbr\u003e\n5-hydroxymethylcytosine (5-hmC)\u003cbr\u003e\n, has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.\u003cbr\u003e\nHydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.\u003cbr\u003e\nIt can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (\u003cbr\u003e\nab117129\u003cbr\u003e\n), which specifically quantifies\u003cbr\u003e\n, for simultaneously quantification of both methylated and hydroxymethylated DNA.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843610103977,"sku":"ab117131","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab117131","provider":"Iright","version":"1.0","type":"link"}