{"product_id":"abcam-ab119599","title":"Abcam, ab119599, Human Laminin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Laminin ELISA Kit is a sandwich ELISA designed to quantify Human Laminin with a sensitivity of 10 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 156 - 10000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Serum, Cell Lysate, Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 10 pg\/mL,\u003cbr\u003e\nRange:156 - 10000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Laminin ELISA Kit ab119599 is a sandwich ELISA to measure Human Laminin in serum, cell culture supernatant, cell lysate with a sensitivity of 10 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA Laminin specific rabbit polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from rabbit specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates ware washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Laminin amount of sample captured in plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman Laminin ELISA Kit ab119599 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate 37C\u003cbr\u003e\n2. Add prepared biotin antibody to each well. Incubate at 37C\u003cbr\u003e\n3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C\u003cbr\u003e\n4. Add TMB to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450 nm\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLaminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLaminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nLaminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.\u003cbr\u003e\nLaminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843640676521,"sku":"ab119599","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab119599","provider":"Iright","version":"1.0","type":"link"}