{"product_id":"abcam-ab123545","title":"Abcam, ab123545, MitoBiogenesis™ Western Blot Cocktail","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMitoBiogenesis™ Western Blot Cocktail (ab123545) is part of the reagents, controls \u0026amp; accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.\u003cbr\u003e\nCocktail Antibodies:\u003cbr\u003e\nMouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal\u003cbr\u003e\nAmount: 25 μg\u003cbr\u003e\nWorking concentration: 0.5 μg\/ml\u003cbr\u003e\nMouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal\u003cbr\u003e\nAmount: 50 μg\u003cbr\u003e\nWorking concentration: 1 μg\/ml\u003cbr\u003e\nMouse Beta actin [mAbcam 8224] monoclonal\u003cbr\u003e\nAmount: 25 μg\u003cbr\u003e\nWorking concentration: 0.5 μg\/ml\u003cbr\u003e\n**Antibody Purity:** Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe combined target of SDHA COX1 and Beta-Actin involves three proteins with distinct mechanical roles in the cell. SDHA also known as succinate dehydrogenase subunit A exhibits a mass of approximately 70 kDa and functions as part of the mitochondrial complex II. COX1 or cytochrome c oxidase subunit 1 weighs around 57 kDa and is integral to the electron transport chain. Beta-Actin a cytoskeletal protein with a mass of about 42 kDa maintains cellular structure. SDHA and COX1 are expressed in mitochondria whereas Beta-Actin is found in the cytoplasm of most eukaryotic cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSDHA helps convert succinate to fumarate in the Krebs cycle also known as the citric acid cycle as part of mitochondrial complex II. COX1 facilitates the transfer of electrons from cytochrome c to oxygen in mitochondrial complex IV. Beta-Actin plays roles in maintaining cell shape enabling cell motility and facilitating intracellular transport. Together SDHA and COX1 are involved in mitochondrial activities essential for energy production while Beta-Actin supports structural integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nSDHA and COX1 significantly contribute to oxidative phosphorylation and the Krebs cycle important for energy metabolism. SDHA interacts with fumarate hydratase and succinate-coenzyme Q reductase in energy production pathways whereas COX1 works synergistically with other cytochrome c oxidase subunits. Beta-Actin is less involved in metabolic pathways and more associated with pathways governing cell movement and division interacting with proteins like myosin and tropomyosin.\u003cbr\u003e\nSDHA and COX1 are implicated in mitochondrial dysfunctions such as Leigh syndrome and mitochondrial complex II deficiency. These disorders often lead to compromised energy metabolism and neurological symptoms. Beta-Actin abnormalities might relate to cancer metastasis due to its role in cell movement and structure. Disrupted interactions between these proteins and their pathways can result in altered cellular energy production and structural organization exacerbating disease conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847572443305,"sku":"ab123545","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab123545","provider":"Iright","version":"1.0","type":"link"}