{"product_id":"abcam-ab125560","title":"Abcam, ab125560, Recombinant human PDK4 protein","description":"\u003cp\u003eSize: 5µg \/ 10µg\u003cbr\u003e\nRecombinant human PDK4 protein is a Human Full Length protein, in the 1 to 411 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;90%, suitable for SDS-PAGE, WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% Densitometry,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:WB, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of ab125560 is 20 nmol\/min\/mg.,\u003cbr\u003e\nAccession:Q16654,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab125602\u003cbr\u003e\n(Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPDK4 also known as Pyruvate Dehydrogenase Kinase Isozyme 4 plays an essential role in regulating mitochondrial energy metabolism. PDK4 phosphorylates the pyruvate dehydrogenase complex controlling the transformation of pyruvate to acetyl-CoA a critical step in energy production. The protein has a molecular weight of around 49 kDa. PDK4 expression occurs in various tissues with high levels observed in skeletal muscle heart and liver reflecting its role in energy metabolic processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme functions to inhibit the pyruvate dehydrogenase complex by adding phosphate groups which reduces the conversion of pyruvate to acetyl-CoA and shifts energy production from carbohydrates to fats especially during fasting or prolonged exercise. PDK4 operates as a homodimer and is often part of a larger regulatory system balancing glucose and fatty acid oxidation in response to nutrient availability.\u003cbr\u003e\nPathways\u003cbr\u003e\nPDK4 is integral to the regulation of both glucose metabolism and fatty acid oxidation. The enzyme interacts closely with the insulin signaling pathway where it modulates glucose uptake and utilization based on hormonal signals. Additionally PDK4 is a part of the hypoxia-inducible factor-1 (HIF-1) pathway where it plays a role in cellular response to low oxygen levels interacting with related proteins such as pyruvate dehydrogenase (PDH).\u003cbr\u003e\nPDK4's activity is linked to metabolic conditions like diabetes and obesity where its altered expression can lead to insulin resistance by affecting glucose utilization. Furthermore PDK4 is associated with cardiovascular diseases through its influence on cardiac energy metabolism and its interaction with other proteins such as PDH contributing to heart dysfunction when deregulated.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843846394025,"sku":"ab125560","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab125560","provider":"Iright","version":"1.0","type":"link"}