{"product_id":"abcam-ab125602","title":"Abcam, ab125602, Recombinant Human PDHA1 protein","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant Human PDHA1 protein is a Human Full Length protein, in the 30 to 390 aa range, expressed in Escherichia coli, with \u0026gt;85%, suitable for SDS-PAGE, WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;85% Densitometry,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:WB, FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P08559,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.81% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for the following active protein Kinases:\u003cbr\u003e\nab125560\u003cbr\u003e\n(Active human PDK4 full length protein)\u003cbr\u003e\nab125580\u003cbr\u003e\n(Active human Mitochondrial Pyruvate dehydrogenase kinase 1 full length protein)\u003cbr\u003e\nab125592\u003cbr\u003e\n(Active human PDK2 full length protein)\u003cbr\u003e\nab125606\u003cbr\u003e\n(Active human PDK3 full length protein)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.\u003cbr\u003e\nPathways\u003cbr\u003e\nPDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.\u003cbr\u003e\nMutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843908391081,"sku":"ab125602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab125602","provider":"Iright","version":"1.0","type":"link"}