{"product_id":"abcam-ab126785","title":"Abcam, ab126785, Anti-CYP27A1 antibody [EPR7529]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-CYP27A1 antibody [EPR7529] (ab126785) is a rabbit monoclonal antibody detecting CYP27A1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7529,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CYP27A1 antibody [EPR7529] (ab126785) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of CYP27A1?\u003cbr\u003e\nAnti-CYP27A1 [EPR7529] (ab126785) specifically detects a band for CYP27A1 (UniProt: Q02318) at a molecular weight of 60kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CYP27A1 [EPR7529] (ab126785) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-CYP27A1 [EPR7529] (ab126785) has over 5 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR7529] also available for your convenience: ab126785, Carrier free -\u003cbr\u003e\nab214800\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305862\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305863\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305864\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308834\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab309770\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab310141\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310565\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312094\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312574\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321015\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCYP27A1 also known as sterol 27-hydroxylase is a member of the cytochrome P450 superfamily. It functions as a mitochondrial enzyme with a mass of approximately 59 kDa localized in the liver and various other tissues. CYP27A1 hydroxylates its substrates playing an important role in cholesterol metabolism. This process involves the conversion of cholesterol to bile acids an essential step in enabling the excretion of cholesterol from the body. High expression levels of CYP27A1 can be observed in hepatic tissue reflecting its central role in lipid metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCYP27A1 participates in cholesterol catabolism. While it does not form large protein complexes it interacts with other enzymes in metabolic pathways. This enzyme initiates the acidic pathway of bile acid synthesis by converting cholesterol into 27-hydroxycholesterol. These transformations are important for maintaining cholesterol homeostasis and for producing bile acids that aid in fat digestion and nutrient absorption.\u003cbr\u003e\nPathways\u003cbr\u003e\nCYP27A1 is central to the bile acid biosynthesis and cholesterol metabolism pathways. It collaborates with other enzymes such as CYP7A1 which is known for initiating the classic pathway of bile acid synthesis. Together they regulate cholesterol levels by metabolizing cholesterol into bile acids. Such pathways are critical in preventing cholesterol accumulation which could lead to various metabolic disorders.\u003cbr\u003e\nImpairment of CYP27A1 function links to cerebrotendinous xanthomatosis (CTX) marked by the accumulation of cholestanol in tissues leading to neurological dysfunction. Mutations in the CYP27A1 gene disrupt cholesterol metabolism resulting in the accumulation of cholesterol derivatives. Furthermore there is a connection to cardiovascular disease due to the role of CYP27A1 in maintaining cholesterol balance linked with proteins like ApoE impacting lipid transport.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844064858281,"sku":"ab126785","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab126785","provider":"Iright","version":"1.0","type":"link"}