{"product_id":"abcam-ab132351","title":"Abcam, ab132351, Recombinant Human USP1 protein (Tagged)","description":"\u003cp\u003eSize: 2µg\u003cbr\u003e\nRecombinant Human USP1 protein (Tagged) is a Human Full Length protein, in the 1 to 785 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nExpression system:Wheat germ,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:O94782,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nUSP1 also known as ubiquitin-specific peptidase 1 is a deubiquitinating enzyme with a molecular mass of approximately 88 kDa. It removes ubiquitin from protein substrates especially those involved in DNA repair processes. USP1 is widely expressed in human tissues with high expression in the testis and moderate levels in the lungs brain and heart. It forms a complex with UAF1 which enhances its catalytic activity and stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nUSP1 plays a critical part in maintaining genomic stability by regulating key proteins in the DNA damage response. It specifically deubiquitinates FANCD2 and PCNA proteins important for the Fanconi anemia pathway and translesion DNA synthesis respectively. This action prevents the accumulation of ubiquitinated proteins ensuring proper progression of DNA repair processes. The interaction with UAF1 forms a functional complex important for its activity in vivo.\u003cbr\u003e\nPathways\u003cbr\u003e\nUSP1 is significant in the Fanconi anemia (FA) and translesion synthesis pathways. Its interaction with FANCD2 involves DNA repair by promoting error-free replication across DNA lesions. USP1 also acts on proliferating cell nuclear antigen (PCNA) a protein important for translesion synthesis that enables DNA replication to occur past unrepaired lesions. These pathways highlight USP1’s importance in DNA repair and replication linking it to other proteins like RAD18 which is involved in PCNA ubiquitination.\u003cbr\u003e\nResearch implicates USP1 in cancer and Fanconi anemia. In certain cancers altered USP1 expression can lead to increased DNA repair activity contributing to chemoresistance. USP1’s interaction with FANCD2 makes it pertinent to Fanconi anemia where its malfunction can cause DNA repair defects associated with the disease. Understanding USP1’s role alongside proteins like BRCA1 which interacts with DNA damage repair mechanisms can guide targeted therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843909111977,"sku":"ab132351","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab132351","provider":"Iright","version":"1.0","type":"link"}