{"product_id":"abcam-ab133615","title":"Abcam, ab133615, Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker","description":"\u003cp\u003eSize: 10µL-TRIAL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) is a rabbit monoclonal antibody detecting Calnexin in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 70 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3633(2),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Recognizes ER membrane, mitochondria and cis-Golgi.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), IHC-P, WB in human samples.\u003cbr\u003e\nAnti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) has been cited over 78 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (ab133615) specifically detects Calnexin (UniProt ID: P27824; Molecular weight: 65kDa) and is sold in 100 µL and 1 mL selling sizes.\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EPR3633(2) -\u003cbr\u003e\nab225542\u003cbr\u003e\nAntibody clone EPR3633(2) is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 594, APC, PE, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 568, Alexa Fluor® 555 (\u003cbr\u003e\nab195198\u003cbr\u003e\nab203439\u003cbr\u003e\nab310822\u003cbr\u003e\nab310899\u003cbr\u003e\nab310982\u003cbr\u003e\nab311104\u003cbr\u003e\nab312999\u003cbr\u003e\nab313204\u003cbr\u003e\nReferences regarding specificity:\u003cbr\u003e\nHorner SM\u003cbr\u003e\net al.\u003cbr\u003e\nMitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus.\u003cbr\u003e\nProc Natl Acad Sci U S A\u003cbr\u003e\n108:14590-5 (2011). PubMed: 21844353\u003cbr\u003e\nMyhill N\u003cbr\u003e\net al.\u003cbr\u003e\nThe subcellular distribution of calnexin is mediated by PACS-2.\u003cbr\u003e\nMol Biol Cell\u003cbr\u003e\n19:2777-88 (2008). PubMed: 18417615\u003cbr\u003e\nYoshimura SI\u003cbr\u003e\net al.\u003cbr\u003e\nDirect targeting of cis-Golgi matrix proteins to the Golgi apparatus.\u003cbr\u003e\nJ Cell Sci\u003cbr\u003e\n114:4105-15 (2001). PubMed: 11739642\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCalnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCalnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.\u003cbr\u003e\nPathways\u003cbr\u003e\nCalnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.\u003cbr\u003e\nCalnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844032745641,"sku":"ab133615","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab133615","provider":"Iright","version":"1.0","type":"link"}