{"product_id":"abcam-ab133734","title":"Abcam, ab133734, Anti-PTBP1 antibody [EPR9048(B)]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-PTBP1 antibody [EPR9048(B)] (ab133734) is a rabbit monoclonal antibody detecting PTBP1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR9048(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WB, ICC\/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-PTBP1 antibody [EPR9048(B)] (ab133734) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse samples.\u003cbr\u003e\nWhat is the molecular weight of PTBP1?\u003cbr\u003e\nAnti-PTBP1 [EPR9048(B)] (ab133734) specifically detects a band for PTBP1 (UniProt: P26599) at a molecular weight of 57kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-PTBP1 [EPR9048(B)] (ab133734) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-PTBP1 antibody [EPR9048(B)] (ab133734) has been confirmed by Western blot testing in PTBP1 Knockout HAP1 cell line,\u003cbr\u003e\nab265155\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR9048(B)] also available for your convenience: ab133734, Alexa Fluor® 647 -\u003cbr\u003e\nab201020\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab240079\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305838\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305839\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305840\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308831\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab309768\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310562\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312091\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312571\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321066\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPTBP1 also known as polypyrimidine tract-binding protein 1 or hnRNP I is a protein involved in RNA binding. It weighs approximately 57 kDa and is known for its expression in diverse cell types especially in the nucleus of eukaryotic cells. PTBP1 contains four RNA recognition motifs which allow it to interact with polypyrimidine-rich sequences in pre-mRNA. This interaction plays an important role in RNA splicing and the regulation of alternative splicing events.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPTBP1 influences the stability transport and translation of mRNA molecules. It is part of the heterogeneous nuclear ribonucleoprotein complex which plays a fundamental role in the processing and modification of pre-mRNA into mature mRNA. This activity impacts the expression of a wide range of genes affecting cellular function and growth. PTBP1 regulates mRNA metabolism contributing significantly to post-transcriptional gene regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of PTBP1 spans the management of alternative splicing and the modulation of gene expression. It participates importantly in the alternative splicing pathway alongside proteins like PTBP2 which perform overlapping functions in neurons. PTBP1 affects exon inclusion or exclusion influencing the genetic diversity presented in protein forms within cells. It also plays a role in the exon junction complex pathway impacting post-translational modifications and protein interactions.\u003cbr\u003e\nVariations in PTBP1 function are linked to conditions such as cancer and neurological disorders. Altered expression levels or mutations can contribute to carcinogenesis through the misregulation of splice variants specific to oncogenes and tumor suppressor genes. In neurological disorders PTBP1 interacts with proteins like FUS affecting the regulation of neuronal-specific alternative splicing. Misbalance in these interactions can have significant pathological implications contributing to diseases such as amyotrophic lateral sclerosis (ALS) and glioblastomas.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855734919337,"sku":"ab133734","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab133734","provider":"Iright","version":"1.0","type":"link"}