{"product_id":"abcam-ab134001","title":"Abcam, ab134001, Human Apoptosis Antibody Array - Membrane (43 Targets)","description":"\u003cp\u003eSize: 1 x 2Membranes \/ 1 x 4Membranes \/ 1 x 8Membranes\u003cbr\u003e\nHuman Apoptosis Antibody Array - Membrane (43 Targets) ab134001 is a membrane based multiplex immunoassay for the simultaneous detection of 43 human apoptotic markers in all sample types. - More data, less sample: screen multiple apoptotic markers with the same sample - Global view of apoptosis - Chemiluminescent detection - no specific equipment required - Highly sensitive (pg\/mL)\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nTarget:BADSee target data,\u003cbr\u003e\nApplications:Multiplex Protein DetectionSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSample types:Cell culture supernatant, Plasma, Serum\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab134001 is for simultaneous detection of 43 Human Apoptotic markers.\u003cbr\u003e\nTargets:\u003cbr\u003e\nBad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP\u003cbr\u003e\nCaspase 3-will detect only the active form\u003cbr\u003e\nCaspase 8-will detect the full length pro-form and the active form.\u003cbr\u003e\nAntibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed\/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.\u003cbr\u003e\nWe do not recommend cell and tissue lysates for the ab134001 as it may mask the positive control spots.\u003cbr\u003e\nUsing tissue or cell lysate samples may mask the positive control spots. This would need additional optimization steps.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847585943721,"sku":"ab134001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab134001","provider":"Iright","version":"1.0","type":"link"}