{"product_id":"abcam-ab135015","title":"Abcam, ab135015, Recombinant E. coli BirA protein (Active)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant E. coli BirA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 2 to 321 aa range, expressed in Escherichia coli, with \u0026gt;65%, suitable for SDS-PAGE, WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;65% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His-DDDDK tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:ab135015 (10 μg\/ml) completely biotinylated 10 μM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 μM d-Biotin.,\u003cbr\u003e\nAccession:P06709,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Escherichia coli K-12,\u003cbr\u003e\nStorage buffer:pH: 8Preservative: 1.02% ImidazoleConstituents: 5% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nUseful for covalently biotin-labeling of acceptor proteins for detection and purification assays.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein BirA also known as the biotin protein ligase (BPL) serves an important role in biotinylation. Mechanically BirA activates biotin through ATP-dependent reactions linking it to proteins. The BirA protein has an approximate molecular weight of 35 kDa a characteristic referred to as the BirA molecular weight or BirA protein size. BirA is typically expressed in bacterial cells with Escherichia coli being a primary model organism for studying its activity. In its active form BirA functions as a homodimer a stable complex required for its catalytic activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nBirA adds biotin to specific lysine residues on target proteins a modification important for protein function. This process often regulates proteins involved in metabolism and gene expression. BirA does not operate as a part of a larger protein complex but acts on its own enzyme-substrate interactions. Its distribution is notable in prokaryotic cells where it plays essential roles in microbial biotin metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nBirA significantly interacts in the biotin metabolic pathway vital for fatty acid synthesis and gluconeogenesis. The protein's ability to biotinylate targets is vital in pathways where it helps regulate enzymes like acetyl-CoA carboxylase and pyruvate carboxylase which are critical for lipid and carbohydrate metabolism. In the biotin regulation network BirA interacts with other proteins including the biotin repressor protein BioR for controlling gene expression related to biotin and related pathways.\u003cbr\u003e\nDeficiencies in biotin metabolism pathways where BirA activity is central can lead to conditions such as biotinidase deficiency and phenylketonuria (PKU). Disruptions in these pathways often result from inadequate biotinylation where BirA plays a critical role. Although not directly linked to human diseases the homologs of BirA studied in human cells are relevant for understanding the molecular basis of metabolic disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843854979241,"sku":"ab135015","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab135015","provider":"Iright","version":"1.0","type":"link"}