{"product_id":"abcam-ab135395","title":"Abcam, ab135395, HeLa Ser\/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated","description":"\u003cp\u003eSize: 2Unit\u003cbr\u003e\nHeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nHeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine\/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.\u003cbr\u003e\nConcentration:\u003cbr\u003e\nHeLa Lysate - Vehicle Treated Control - 200 μg at 1 mg\/mL\u003cbr\u003e\nHeLa Lysate - Calyculin Treated - 200 μg at 1 mg\/mL\u003cbr\u003e\nThe lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847602196649,"sku":"ab135395","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab135395","provider":"Iright","version":"1.0","type":"link"}