{"product_id":"abcam-ab136761","title":"Abcam, ab136761, Rabbit F(ab')2 Anti-Pig IgG H\u0026L (HRP)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nSuitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nTarget species:Pig,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:HRP,\u003cbr\u003e\nApplications:IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPreparation of Substrate Solutions:\u003cbr\u003e\n- ELISA: OPD (orthophenylene diamine): 0.4mg\/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl\/ml H\u003cbr\u003e\n(30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V\/2 volume of 1M H\u003cbr\u003e\n. Reading at 492nm\u003cbr\u003e\n- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg\/ml in a 0.1M pH7.4 TRIS\/HCl buffer + 5µl\/ml H\u003cbr\u003e\n(30% hydrogen peroxide solution). Incubation 10 minutes in darkness.\u003cbr\u003e\n- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl\/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H\u003cbr\u003e\n. Incubate 10 min at room temperature.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136761 is purified by affinity chromatography and is thus particularly specific., Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847551013033,"sku":"ab136761","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab136761","provider":"Iright","version":"1.0","type":"link"}