{"product_id":"abcam-ab136957","title":"Abcam, ab136957, Hexokinase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nHexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure for the measurement of hexokinase activity. Readout on any colorimetric (450 m) plate reader. - Complete kit including standard curve for quantitation - Cited in \u0026gt; 50 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.1 mU\/well,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.\u003cbr\u003e\nHexokinase activity assay protocol summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix and incubate for 20-60 min\u003cbr\u003e\n- Analyze repeatedly with a microplate reader during the incubation\u003cbr\u003e\nFor optimal enzymatic activity samples should not contain EDTA or other chelating agents.\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nHexokinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:\u003cbr\u003e\nMouse parotid salivary gland samples\u003cbr\u003e\nRat gastrocnemius muscle tissues\u003cbr\u003e\nHuman breast cancer cell lines\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Buss L et al. 2024\u003cbr\u003e\n2-Shakoor H al. 2023\u003cbr\u003e\n3-Mathur D et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nFor fluorometric detection, we recommend Hexokinase Activity Assay Kit (Fluorometric)\u003cbr\u003e\nab211103\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.\u003cbr\u003e\nHexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843598569641,"sku":"ab136957","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab136957","provider":"Iright","version":"1.0","type":"link"}