{"product_id":"abcam-ab139443","title":"Abcam, ab139443, MMP1 Inhibitor Screening Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMMP1 Inhibitor Screening Assay Kit (Colorimetric) (ab139443) is a complete assay system designed to screen MMP1 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Inhibitor compounds,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMMP1 Inhibitor Screening Assay Kit (Colorimetric) (ab139443) is a complete assay system designed to screen MMP1 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC\u003cbr\u003e\n). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M\u003cbr\u003e\nat pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.\u003cbr\u003e\nThis kit is useful to screen inhibitors of MMP1, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.\u003cbr\u003e\nThiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nStudies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.\u003cbr\u003e\nMMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843643232425,"sku":"ab139443","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab139443","provider":"Iright","version":"1.0","type":"link"}