{"product_id":"abcam-ab15149","title":"Abcam, ab15149, Anti-6X His tag® antibody [AD1.1.10]","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nAnti-6X His tag® antibody [AD1.1.10] (ab15149) is a mouse monoclonal antibody detecting 6X His tag® in  Western Blot, ICC\/IF, ELISA . - Recombinant format for unrivalled batch-batch consistency - Trusted since 2004\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:AD1.1.10,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:I-ELISA, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-6X His tag® antibody [AD1.1.10] (ab15149) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry\/immunofluorescence (ICC\/IF), ELISA.\u003cbr\u003e\nTagged protein detection\u003cbr\u003e\n6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-6X His tag® [AD1.1.10] (ab15149) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE -\u003cbr\u003e\nab72467\u003cbr\u003e\n, SureLight® APC -\u003cbr\u003e\nab72579\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab81652\u003cbr\u003e\n, PerCP -\u003cbr\u003e\nab117496\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab117504\u003cbr\u003e\n, DyLight® 550 -\u003cbr\u003e\nab117507\u003cbr\u003e\n, DyLight® 488 -\u003cbr\u003e\nab117512\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab178563\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab307236\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nHIS-TAG® is a trademark of EMD Biosciences, Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.\u003cbr\u003e\nThe 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848169869481,"sku":"ab15149","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab15149","provider":"Iright","version":"1.0","type":"link"}