{"product_id":"abcam-ab154538","title":"Abcam, ab154538, Anti-TMEM66\/SARAF antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal TMEM66\/SARAF antibody. Suitable for IHC-P, WB, ICC\/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SARAF aa 1-250.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human SARAF aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q96BY9\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM66 also known as SARAF acts as a negative regulator of store-operated calcium entry (SOCE). It plays a role in maintaining cellular calcium homeostasis by limiting calcium influx. TMEM66 has a mass of approximately 36 kDa and is encoded by the SARAF gene. It is primarily expressed in the endoplasmic reticulum membrane but can also localize to the plasma membrane depending on the cellular context. It mainly appears in tissues with high metabolic or excitatory activity like the brain and heart.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM66\/SARAF functions to prevent calcium overload in cells by interacting with the calcium release-activated calcium (CRAC) channel components. It acts as a part of the larger store-operated calcium channel complex which includes STIM1 and Orai1. The interaction of TMEM66 with these proteins modulates their activity and ensures a controlled calcium entry into the cell playing a protective role during stimulations that otherwise might cause cellular damage due to excessive calcium levels.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM66\/SARAF directly influences the calcium signaling pathway and is also indirectly involved in the MAPK\/ERK pathway. Calcium signaling is essential for various cellular processes including muscle contraction neurotransmitter release and gene expression. TMEM66 regulates this pathway by modulating Orai1 activity which is a critical component of the calcium influx mechanism. The MAPK\/ERK pathway associated with cell proliferation and differentiation also interacts with calcium levels bridging a connection to TMEM66's role in cellular responses.\u003cbr\u003e\nResearch links TMEM66\/SARAF to cardiac hypertrophy and neurodegenerative diseases. Abnormalities in calcium homeostasis influenced by TMEM66 can lead to pathologies like excess growth of heart muscle cells (cardiac hypertrophy). Additionally disrupted calcium signaling related to TMEM66's dysregulation potentially contributes to neuronal damage seen in neurodegenerative conditions. The protein's interaction with STIM1 and Orai1 underlies these disease connections as disturbances in these proteins' functions can disrupt normal calcium homeostasis potentially leading to cell injury or death.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850146238633,"sku":"ab154538","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab154538","provider":"Iright","version":"1.0","type":"link"}