{"product_id":"abcam-ab155439","title":"Abcam, ab155439, Human SMAC ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman SMAC ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SMAC in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.1 ng\/mL,\u003cbr\u003e\nRange:0.12 - 30 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's SMAC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay designed for the quantitative measurement of Human SMAC in serum, plasma (Human SMAC concentration is pretty low in normal serum\/plasma. It may not be in this assay) and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human SMAC coated on a 96-well plate. Standards and samples are pipetted into the wells and SMAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SMAC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSmac\/Diablo is a mitochondrial protein that promotes apoptosis by interfering with inhibitor of apoptosis proteins (IAPs). The protein also known as Second Mitochondria-derived Activator of Caspases weighs approximately 27 kDa. Researchers detect Smac\/Diablo in various tissues with higher expression levels in organs like the heart and brain. This protein's expression also varies depending on the cell's state and external stimuli.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSmac\/Diablo plays a significant role in programmed cell death by binding to IAPs and negating their inhibition of caspases the key executors of apoptosis. Smac\/Diablo releases from mitochondria into the cytosol when apoptotic signals activate. It does not form complexes but interacts with IAPs facilitating the activation of caspases and enhancing the apoptotic response. Its interaction with IAPs highlights its important function in apoptosis regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nSmac\/Diablo integrates into the mitochondrial apoptosis pathway contributing to the intrinsic pathway of apoptosis. It closely interacts with proteins such as caspases and IAPs. This integration is important for apoptosis regulation linking mitochondrial outer membrane permeabilization with the activation of caspases. Additionally the protein engages in the cytochrome c pathway promoting apoptosis by restricting IAPs and aiding in the release of cytochrome c important for apoptosis progression.\u003cbr\u003e\nResearchers associate Smac\/Diablo with cancer and neurodegenerative diseases like Alzheimer's disease. In cancer elevated Smac\/Diablo levels can result in increased apoptotic death of cancerous cells potentially serving as a target for therapy. It also interacts with proteins like XIAP in these disease contexts. In neurodegenerative disorders dysregulation of apoptosis pathways involving Smac\/Diablo might contribute to excessive neuronal cell death. Understanding Smac\/Diablo's role in these diseases provides insights into possible therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855662534825,"sku":"ab155439","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab155439","provider":"Iright","version":"1.0","type":"link"}