{"product_id":"abcam-ab155444","title":"Abcam, ab155444, Human TSLP ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman TSLP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TSLP in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 3 pg\/mL,\u003cbr\u003e\nRange:3.3 - 800 pg\/mL,\u003cbr\u003e\nAssay time:5h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's TSLP Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed the quantitative measurement of Human TSLP in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human TSLP coated on a 96-well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nGet higher sensitivity in only 90 minutes with Human TSLP ELISA Kit (\u003cbr\u003e\nab192149\u003cbr\u003e\n) from our SimpleStep ELISA\u003cbr\u003e\nrange.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nA mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.\u003cbr\u003e\nTSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843653095593,"sku":"ab155444","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab155444","provider":"Iright","version":"1.0","type":"link"}