{"product_id":"abcam-ab155763","title":"Abcam, ab155763, Anti-OPRT antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal UMPS antibody. Suitable for IHC-P, WB, ICC\/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UMPS aa 200 to C-terminus.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human UMPS aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P11172\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOrotate phosphoribosyltransferase often abbreviated as OPRT or known by its enzyme commission number EC 2.4.2.10 plays a significant role in pyrimidine biosynthesis. This enzyme with a molecular weight of around 23 kDa catalyzes the conversion of orotate and 5-phosphoribosyl-1-pyrophosphate (PRPP) into orotidine-5'-monophosphate (OMP). OPRT is expressed in various tissues with notably higher levels in rapidly proliferating cells due to their increased demand for nucleotides.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOrotate phosphoribosyltransferase contributes to the de novo synthesis of pyrimidine nucleotides which are essential components of DNA and RNA. OPRT operates as part of the UMP biosynthesis via the de novo pathway forming a complex with orotidine-5'-phosphate decarboxylase in some organisms. This linkage enhances efficiency in pyrimidine nucleotide production reflecting its central role in cell growth and division.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe protein integrates into the pyrimidine metabolism pathway. It interacts closely with orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase forming dual-function enzymes that streamline UMP synthesis. OPRT’s activity ties it to key metabolic cycles including nucleotide salvage pathways thereby establishing critical links across metabolic networks essential for nucleic acid production.\u003cbr\u003e\nDysfunction in Orotate phosphoribosyltransferase can lead to hereditary orotic aciduria a rare autosomal recessive disorder characterized by megaloblastic anemia and excessive orotate excretion. Changes in OPRT activity also impact pyrimidine antimetabolite drug efficacy such as 5-fluorouracil demonstrating its significance in cancer treatment. Additionally OPRT's interactions with dihydroorotate dehydrogenase can be important in understanding metabolic disturbances related to pyrimidine disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854050709673,"sku":"ab155763","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab155763","provider":"Iright","version":"1.0","type":"link"}