{"product_id":"abcam-ab155900","title":"Abcam, ab155900, Xanthine\/Hypoxanthine Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nXanthine\/Hypoxanthine Assay kit (ab155900) is a product where Xanthine\/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer \u0026amp; Probe to form a product that can be measured colorimetrically (570 nm) or fluorometrically (Ex\/Em = 535\/587 nm). Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue, Suspension cells, Tissue Homogenate, Cell culture supernatant, Milk, Serum, Other biological fluids, Adherent cells,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 0.4 µM,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nXanthine\/Hypoxanthine Assay kit (ab155900) is a product where Xanthine\/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer \u0026amp; Probe to form a product that can be measured colorimetrically (570 nm) or fluorometrically (Ex\/Em = 535\/587 nm). Xanthine\/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 uM in various biological samples.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K685 Biovision Xanthine\/Hypoxanthine Colorimetric\/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nXanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for diagnostic and therapeutic monitoring.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXanthine and hypoxanthine are purine derivatives and key intermediates in the purine degradation pathway. They play a critical role in catabolism by converting hypoxanthine to xanthine which subsequently transforms into uric acid by xanthine oxidase. These compounds are expressed ubiquitously in tissues where purine metabolism is active. Xanthine's molecular mass is 152.11 g\/mol while hypoxanthine has a molecular mass of 136.11 g\/mol. Hypoxanthine is also considered a substrate for the salvage pathways in nucleotide synthesis providing an alternate route to synthesize important nucleotides.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nXanthine and hypoxanthine function as central components in the cellular degradation of purines participating actively in the turnover of nucleic acids. They are not part of any specific protein complex but interact with enzymes like xanthine oxidase which facilitates the oxidation processes. These molecules play an essential role in ensuring the balanced recycling of purine nucleotides which are necessary for cellular energy transactions and genetic material synthesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese molecules are integral to purine metabolism pathways and are linked with the degradation and salvage pathways. Xanthine and hypoxanthine are related through their connection with enzymes such as xanthine oxidase and hypoxanthine-guanine phosphoribosyltransferase which regulate the critical steps of converting them into uric acid and nucleotides. These interactions position them as influential players in maintaining cellular homeostasis and energy balance by recycling purines effectively.\u003cbr\u003e\nXanthine and hypoxanthine have significant links to conditions such as gout and Lesch-Nyhan syndrome. Gout arises from high levels of uric acid formed from xanthine leading to painful joint inflammation. Lesch-Nyhan syndrome connects with hypoxanthine due to a deficiency in hypoxanthine-guanine phosphoribosyltransferase causing an aberrant nucleotide metabolism. These relationships underline the importance of maintaining the correct functional levels of xanthine and hypoxanthine to prevent such metabolic disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843622719657,"sku":"ab155900","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab155900","provider":"Iright","version":"1.0","type":"link"}