{"product_id":"abcam-ab157705","title":"Abcam, ab157705, Guinea Pig Complement C3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nGuinea Pig Complement C3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Guinea Pig Complement C3 in Guinea pig in Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum,\u003cbr\u003e\nReacts with:Guinea pig,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.3083 ng\/mL,\u003cbr\u003e\nRange:3.13 - 3260 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Complement C3 Guinea Pig ELISA Kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Complement C3 levels in serum and blood .\u003cbr\u003e\nIn this assay the Complement C3 present in samples reacts with the anti-Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nComplement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.\u003cbr\u003e\nComplement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843645788329,"sku":"ab157705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab157705","provider":"Iright","version":"1.0","type":"link"}