{"product_id":"abcam-ab157707","title":"Abcam, ab157707, Human Hemoglobin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Hemoglobin ELISA Kit is a Sandwich ELISA used to quantify Human Hemoglobin with a sensitivity of 0.8445 ng\/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 6.25 - 200 ng\/ml - Cited in over 20 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.8445 ng\/mL,\u003cbr\u003e\nRange:6.25 - 200 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Hemoglobin ELISA Kit ab157707 is a sandwich ELISA to measure human hemoglobin in serum and plasma with a sensitivity of 0.8445 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity of Hemoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nInterferences : Azide and thimerosal at concentrations higher than 0.1% inhibits the enzyme reaction.\u003cbr\u003e\nHuman Hemoglobin ELISA Kit ab157707 protocol summary\u003cbr\u003e\n1. Add samples and standards to wells. Incubate at room temperature.\u003cbr\u003e\n2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.\u003cbr\u003e\n3. Wash each well and add Streptavidin Solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nHow other researchers are using Human Hemoglobin ELISA kit ab157707\u003cbr\u003e\nHuman Hemoglobin ELISA kit ab157707 has been used to determine hemoglobin concentration cell free in neonates undergoing cardiopulmonary bypass\u003cbr\u003e\n, and to study links between microglia and complement cascade in Huntingdon's disease\u003cbr\u003e\nReferences:\u003cbr\u003e\nA. Jungner et al., 2022, PMID: 35887835,\u003cbr\u003e\nD.K Wilton et al. 2023, PMID:37814059\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Hemoglobin ELISA kit 10157707 is commonly used for studying blood conditions such as anemia. Other ELISA kits used to measure blood proteins include :\u003cbr\u003e\n- Human Erythropoietin ELISA kit\u003cbr\u003e\nab274397\u003cbr\u003e\n- Human Transferrin ELISA kit\u003cbr\u003e\nab187391\u003cbr\u003e\n- Human Haptoglobin ELISA kit\u003cbr\u003e\nab309180\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.\u003cbr\u003e\nPathways\u003cbr\u003e\nHemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.\u003cbr\u003e\nHemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843611381929,"sku":"ab157707","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab157707","provider":"Iright","version":"1.0","type":"link"}