{"product_id":"abcam-ab167718","title":"Abcam, ab167718, Recombinant human HMGB1 protein","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human HMGB1 protein is a Human Full Length protein, in the 1 to 215 aa range with \u0026gt;95% purity, \u0026lt; 1 EU\/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab167718 protein is 25.7 kDa.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The EC50 for this effect is 0.7855‐0.8342 μg\/ml (Routinely tested).,\u003cbr\u003e\nAccession:P09429,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:It is recommended to reconstitute the lyophilized protein in 100µl sterile deionized water to a final concentration of 1mg\/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: PBS\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant Human HMGB1 protein ab167718 as a positive control in SDS-PAGE.\u003cbr\u003e\nThe human HMGB1 protein has a calculated MW of 26.8 kDa. The ab167718 protein migrates at 32 kDa and 35 kDa under reducing (R) condition in SDS-PAGE due to glycosylation.\u003cbr\u003e\nIn functional ELISA the immobilized Human HMGB1 protein ab167718 at 5 μg\/mL (100 µL\/well) can bind Human RAGE, Fc Tag with a linear range of 0.078-2.5 µg\/mL\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nCheck out our ELISA protocol for more information\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843798323369,"sku":"ab167718","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab167718","provider":"Iright","version":"1.0","type":"link"}