{"product_id":"abcam-ab169558","title":"Abcam, ab169558, Glycogen Assay Kit II (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. - Simple and fast protocol with results within one hour - Can detect 4 - 40 µg\/ml of glycogen in samples - Cited in \u0026gt; 50 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 4 µg\/mL,\u003cbr\u003e\nRange:4 - 40 µg\/mL,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. This kit uses a different readout chemistry to our most popular Glycogen Assay Kit\u003cbr\u003e\nab65620\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn this glycogen assay protocol, glycogen is hydrolyzed into glucose using glucoamylase, glucose is then oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm.\u003cbr\u003e\nGlycogen assay protocol summary:\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add hydrolysis enzyme and incubate for 30 min at room temp\u003cbr\u003e\n- Add reaction mix and incubate for 30 min at room temp\u003cbr\u003e\n- Analyze with microplate reader\u003cbr\u003e\nHow other researchers are using Glycogen Assay Kit ab169558\u003cbr\u003e\nGlycogen Assay Kit II has been used in a variety of sample types including:\u003cbr\u003e\nRat hepatic tissue homogenates\u003cbr\u003e\nHuman chondrosarcoma cells\u003cbr\u003e\nMouse adipose tissue samples\u003cbr\u003e\nReferences:\u003cbr\u003e\n1-Bzdega W et al. 2023\u003cbr\u003e\n2-Pathmanapan S et al. 2023\u003cbr\u003e\n3- Liebscher G et al. 2022\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.\u003cbr\u003e\nGlycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843541356713,"sku":"ab169558","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab169558","provider":"Iright","version":"1.0","type":"link"}